################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BclI BfrI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Alw44I 1477 GTGCAC G^TGCAC AocI 1117 CCTCAGG CC^T[ACGT]AGG ApaLI 1477 GTGCAC G^TGCAC Asp700I 1434 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1477 GTGCAC G[AT]GC[AT]^C AspI 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 512 CCCGGG C^[CT]CG[GA]G BamHI 936 GGATCC G^GATCC BbrPI 1484 CACGTG CAC^GTG BbvCI 974 CCTCAGC CCTCAGC(-5/-2) BcgI 1290 GCAGTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 426 GGATAC GTATCC(6/5) BglII 64 AGATCT A^GATCT BlpI 1045 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1045 GCTGAGC GC^T[ACGT]AGC BsaAI 1484 CACGTG [CT]AC^GT[GA] BsaI 829 GAGACC GGTCTC(1/5) BsiHKAI 1477 GTGCAC G[AT]GC[AT]^C BsoBI 512 CCCGGG C^[CT]CG[GA]G BspLU11I 1426 ACATGT A^CATGT BspMI 1417 ACCTGC ACCTGC(4/8) BsrBI 313 GAGCGG CCGCTC(-3/-3) BstAPI 174 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1308 GGTGACC G^GT[ACGT]ACC BstPI 1308 GGTGACC G^GT[ACGT]ACC Bsu36I 1117 CCTCAGG CC^T[ACGT]AGG CelII 1045 GCTGAGC GC^T[ACGT]AGC DrdI 1095 GACTGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1117 CCTCAGG CC^T[ACGT]AGG EcoNI 519 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1308 GGTGACC G^GT[ACGT]ACC HincII 754 GTTGAC GT[CT]^[GA]AC HindII 754 GTTGAC GT[CT]^[GA]AC HindIII 1386 AAGCTT A^AGCTT HinfI 912 GAGTC G^A[ACGT]TC PflFI 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 912 GAGTC GAGTC(4/5) PmaCI 1484 CACGTG CAC^GTG PmlI 1484 CACGTG CAC^GTG ScaI 592 AGTACT AGT^ACT SmaI 512 CCCGGG CCC^GGG StuI 47 AGGCCT AGG^CCT Tth111I 478 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 512 CCCGGG C^CCGGG XmaI 512 CCCGGG C^CCGGG XmnI 1434 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1426 ACATGT A^C[GA][CT]GT 1483 ACACGT ApaI 125 GGGCCC GGGCC^C 515 GGGCCC BalI 405 TGGCCA TGG^CCA 1580 TGGCCA BanI 604 GGCACC G^G[CT][GA]CC 774 GGCACC BanII 125 GGGCCC G[GA]GC[CT]^C 515 GGGCCC BglI 259 GCCCATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 641 GCCACTGGGGC DsaI 600 CCGTGG C^C[GA][CT]GG 1472 CCACGG EaeI 405 TGGCCA [CT]^GGCC[GA] 1580 TGGCCA EarI 852 CTCTTC CTCTTC(1/4) 1090 GAAGAG HgaI 89 GACGC GACGC(5/10) 757 GACGC Ksp632I 852 CTCTTC CTCTTC(1/4) 1090 GAAGAG MaeII 985 ACGT A^CGT 1485 ACGT MluNI 405 TGGCCA TGG^CCA 1580 TGGCCA MscI 405 TGGCCA TGG^CCA 1580 TGGCCA NspI 1426 ACATGT [GA]CATG^[CT] 1467 ACATGC PflMI 249 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 370 CCAGCCTTTGG PstI 181 CTGCAG CTGCA^G 282 CTGCAG SfcI 181 CTGCAG C^T[GA][CT]AG 282 CTGCAG TaqI 16 TCGA T^CGA 1299 TCGA Tsp509I 320 AATT ^AATT 357 AATT TthHB8I 16 TCGA T^CGA 1299 TCGA Van91I 249 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 370 CCAGCCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XhoII