(Circular) (Six-base) MAPSORT of: pFN21AB3236.seq Check: 5797 from: 1 to: 6421 pFN21AB3236 6421 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4231 278 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3764 1937 Size: 1827 4594 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 3705 4484 5868 6136 1632 Size: 759 1314 779 1384 268 1917 Fragments arranged by size: 1917 1384 1314 779 759 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 5320 6297 1203 Size: 4117 977 1327 Fragments arranged by size: 4117 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 5150 5150 Size: 6421 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3277 1184 Size: 2093 4328 AlwNI CAG_nnn'CTG Cuts at: 2828 4164 5736 2828 Size: 1336 1572 3513 Fragments arranged by size: 3513 1572 1336 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 2185 2291 2684 3171 3730 3956 4108 6217 Size: 692 106 393 487 559 226 152 2109 Cuts at: 6217 1493 Size: 1697 Fragments arranged by size: 2109 1697 692 559 487 393 226 152 106 AseI AT'TA_AT Cuts at: 160 1032 3020 4975 160 Size: 872 1988 1955 1606 Fragments arranged by size: 1988 1955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 3746 1870 Size: 73 1803 4545 Fragments arranged by size: 4545 1803 73 AvrII C'CTAG_G Cuts at: 2923 2923 Size: 6421 BamHI G'GATC_C Cuts at: 3751 4118 3751 Size: 367 6054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3742 5197 618 Size: 325 217 627 1955 1455 1842 Fragments arranged by size: 1955 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3740 1391 Size: 622 1727 4072 Fragments arranged by size: 4072 1727 622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5450 BbvCI CC'TCA_GC Cuts at: 3450 3450 Size: 6421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 2884 1548 Size: 1336 5085 BfrBI ATG'CAT Cuts at: 2420 2759 3566 2420 Size: 339 807 5275 Fragments arranged by size: 5275 807 339 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5032 136 Size: 107 122 71 631 605 3360 1525 Fragments arranged by size: 3360 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 3462 3838 3462 Size: 376 6045 Bme1580I G_kGCm'C Cuts at: 1790 4482 5638 1790 Size: 2692 1156 2573 Fragments arranged by size: 2692 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3035 3284 1318 Size: 1717 249 4455 Fragments arranged by size: 4455 1717 249 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4152 5110 442 Size: 416 53 83 399 24 2735 958 1753 Fragments arranged by size: 2735 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2931 3402 5081 1239 Size: 1692 471 1679 2579 Fragments arranged by size: 2579 1692 1679 471 Bpu10I CC'TnA_GC Cuts at: 3450 3450 Size: 6421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2544 4546 5411 5709 2544 Size: 2002 865 298 3256 Fragments arranged by size: 3256 2002 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 2478 2849 5084 882 Size: 617 876 103 371 2235 2219 Fragments arranged by size: 2235 2219 876 617 371 103 BsaAI yAC'GTr Cuts at: 493 1204 4212 493 Size: 711 3008 2702 Fragments arranged by size: 3008 2702 711 BsaBI GATnn'nnATC Cuts at: 6415 6415 Size: 6421 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4228 4610 5245 275 Size: 53 83 186 936 2695 382 635 1451 Fragments arranged by size: 2695 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4854 5252 5526 5673 6084 4854 Size: 398 274 147 411 5191 Fragments arranged by size: 5191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6196 Fragments arranged by size: 6196 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2618 1186 Size: 1432 4989 BseYI C'CCAG_C Cuts at: 5624 6120 5624 Size: 496 5925 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3713 1163 Size: 573 1977 3871 Fragments arranged by size: 3871 1977 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2392 3871 4632 5660 1540 Size: 275 186 391 1479 761 1028 2301 Fragments arranged by size: 2301 1479 1028 761 391 275 186 BsiHKAI G_wGCw'C Cuts at: 4482 4567 5638 4482 Size: 85 1071 5265 Fragments arranged by size: 5265 1071 85 BsmI GAATG_Cn' Cuts at: 2761 3158 3932 4025 2761 Size: 397 774 93 5157 Fragments arranged by size: 5157 774 397 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2771 2771 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 3740 4482 4567 5638 1391 Size: 399 223 1727 742 85 1071 2174 Fragments arranged by size: 2174 1727 1071 742 399 223 85 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 4310 6040 4310 Size: 1730 4691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2831 3776 844 Size: 1217 770 945 3489 Fragments arranged by size: 3489 1217 945 770 BsrBI CCG'CTC Cuts at: 1394 1823 3627 4308 1394 Size: 429 1804 681 3507 Fragments arranged by size: 3507 1804 681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4916 5090 65 Size: 1183 3668 174 1396 Fragments arranged by size: 3668 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5065 5252 1700 Size: 110 3255 187 2869 Fragments arranged by size: 3255 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6421 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 4481 5493 4481 Size: 1012 5409 BstAPI GCAn_nnn'nTGC Cuts at: 794 2721 2831 3293 794 Size: 1927 110 462 3922 Fragments arranged by size: 3922 1927 462 110 BstBI TT'CG_AA Cuts at: 2860 5225 2860 Size: 2365 4056 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6421 BstXI CCAn_nnnn'nTGG Cuts at: 2909 2909 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2160 2233 2617 3751 4118 4503 4520 5972 Size: 188 73 384 1134 367 385 17 1452 Cuts at: 5972 6058 6070 6416 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1134 385 384 367 346 188 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4930 Fragments arranged by size: 4930 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5682 Fragments arranged by size: 5682 683 56 BtsI GCAGTG_nn' Cuts at: 3266 3937 4731 4751 3266 Size: 671 794 20 4936 Fragments arranged by size: 4936 794 671 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5428 809 Size: 507 4112 1802 Fragments arranged by size: 4112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2423 3352 3868 Size: 54 1425 50 275 18 593 929 516 Cuts at: 3868 4757 6386 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 929 889 593 516 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2641 2862 3330 3483 3681 4351 1353 Size: 744 544 221 468 153 198 670 3423 Fragments arranged by size: 3423 744 670 544 468 221 198 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5008 5382 5528 387 Size: 1064 138 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2391 2931 3402 3705 4484 5081 5868 Size: 393 759 540 471 303 779 597 787 Cuts at: 5868 6136 1239 Size: 268 1524 Fragments arranged by size: 1524 787 779 759 597 540 471 393 303 268 EcoNI CCTnn'n_nnAGG Cuts at: 2078 3354 2078 Size: 1276 5145 EcoRI G'AATT_C Cuts at: 2684 3730 4108 2684 Size: 1046 378 4997 Fragments arranged by size: 4997 1046 378 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 5568 1678 Size: 3890 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 3331 3363 5062 Size: 32 135 32 970 32 757 32 1699 Cuts at: 5062 5094 5136 5168 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1699 970 757 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3715 3765 4011 669 Size: 988 281 1777 50 246 3079 Fragments arranged by size: 3079 1777 988 281 246 50 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 2114 3147 4020 2114 Size: 1033 873 4515 Fragments arranged by size: 4515 1033 873 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3254 5535 5719 1305 Size: 1949 2281 184 2007 Fragments arranged by size: 2281 2007 1949 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2425 3354 10 Size: 54 1425 343 593 929 3077 Fragments arranged by size: 3077 1425 929 593 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 2513 3075 4379 4738 4897 518 Size: 648 1284 63 562 1304 359 159 2042 Fragments arranged by size: 2042 1304 1284 648 562 359 159 63 MspA1I CmG'CkG Cuts at: 1606 3837 4514 5662 5907 1606 Size: 2231 677 1148 245 2120 Fragments arranged by size: 2231 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4930 Fragments arranged by size: 4930 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NsiI A_TGCA'T Cuts at: 2422 2761 3568 2422 Size: 339 807 5275 Fragments arranged by size: 5275 807 339 NspI r_CATG'y Cuts at: 1132 1229 3779 5324 1132 Size: 97 2550 1545 2229 Fragments arranged by size: 2550 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6421 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6421 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3159 3191 4490 4522 6003 6035 3159 Size: 32 1299 32 1481 32 3545 Fragments arranged by size: 3545 1481 1299 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PstI C_TGCA'G Cuts at: 830 3256 3773 830 Size: 2426 517 3478 Fragments arranged by size: 3478 2426 517 PvuI CG_AT'CG Cuts at: 2001 2392 2001 Size: 391 6030 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SapI GCTCTTCn'nnn_ Cuts at: 2862 2862 Size: 6421 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 3643 4669 6381 3643 Size: 1026 1712 3683 Fragments arranged by size: 3683 1712 1026 SexAI A'CCwGG_T Cuts at: 3717 3717 Size: 6421 SfcI C'TryA_G Cuts at: 826 1045 3252 3769 4904 5585 5776 826 Size: 219 2207 517 1135 681 191 1471 Fragments arranged by size: 2207 1471 1135 681 517 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2559 4525 5426 5688 820 Size: 197 926 616 1966 901 262 1553 Fragments arranged by size: 1966 1553 926 901 616 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 3433 4345 5 Size: 47 3381 912 2081 Fragments arranged by size: 3381 2081 912 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6421 StyI C'CwwG_G Cuts at: 513 1065 2004 2488 2902 2923 2952 3276 3355 Size: 552 939 484 414 21 29 324 79 Cuts at: 3355 3860 513 Size: 505 3074 Fragments arranged by size: 3074 939 552 505 484 414 324 79 29 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 4448 4465 4618 4803 5248 325 Size: 1255 471 2397 17 153 185 445 1498 Fragments arranged by size: 2397 1498 1255 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3641 4667 6379 Size: 274 80 33 51 1443 1664 1026 1712 Cuts at: 6379 96 Size: 138 Fragments arranged by size: 1712 1664 1443 1026 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3577 3609 6381 6413 3577 Size: 32 2772 32 3585 Fragments arranged by size: 3585 2772 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6421 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2323 Size: 6421 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 3171 4550 3171 Size: 1379 5042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4229 276 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI