################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AgeI Alw44I Aor51HI ApaLI AscI AspI AvrII BbeI BbsI BcgI BciVI BglI BlnI BpuAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI Cfr10I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI HpaI KasI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PstI PvuI PvuII RsrII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 1171 GGTACC G^GTACC AclI 2614 AACGTT AA^CGTT AflII 1335 CTTAAG C^TTAAG AflIII 1787 ACGTGT A^C[GA][CT]GT AhdI 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1577 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 966 GGGCCC GGGCC^C AseI 2206 ATTAAT AT^TAAT AsnI 2206 ATTAAT AT^TAAT Asp700I 420 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1171 GGTACC G^GTACC AspEI 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 647 CTCGAG C^[CT]CG[GA]G BamHI 1364 GGATCC G^GATCC BbrPI 817 CACGTG CAC^GTG BbvCI 1480 CCTCAGC CCTCAGC(-5/-2) BclI 2126 TGATCA T^GATCA BfrI 1335 CTTAAG C^TTAAG BglII 2489 AGATCT A^GATCT BsaAI 817 CACGTG [CT]AC^GT[GA] BsoBI 647 CTCGAG C^[CT]CG[GA]G BspDI 860 ATCGAT AT^CGAT BspHI 2802 TCATGA T^CATGA BspMI 1105 GCAGGT ACCTGC(4/8) Bst1107I 1491 GTATAC GTA^TAC BstXI 2594 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1491 GTATAC GTA^TAC ClaI 860 ATCGAT AT^CGAT DraIII 746 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1411 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1992 CCGCGG C^C[GA][CT]GG Eam1105I 282 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2580 GAATTC G^AATTC EcoT14I 587 CCAAGG C^C[AT][AT]GG FbaI 2126 TGATCA T^GATCA HaeII 1979 AGCGCC [GA]GCGC^[CT] HincII 3026 GTCGAC GT[CT]^[GA]AC HindII 3026 GTCGAC GT[CT]^[GA]AC HindIII 125 AAGCTT A^AGCTT KpnI 1171 GGTACC GGTAC^C KspI 1992 CCGCGG CCGC^GG MspA1I 1992 CCGCGG C[AC]G^C[GT]G NspI 779 GCATGT [GA]CATG^[CT] PaeR7I 647 CTCGAG C^TCGAG PflMI 2278 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 817 CACGTG CAC^GTG PmlI 817 CACGTG CAC^GTG PshBI 2206 ATTAAT AT^TAAT Psp1406I 2614 AACGTT AA^CGTT RcaI 2802 TCATGA T^CATGA SacII 1992 CCGCGG CCGC^GG SalI 3026 GTCGAC G^TCGAC SpeI 1275 ACTAGT A^CTAGT SspI 848 AATATT AAT^ATT StyI 587 CCAAGG C^C[AT][AT]GG Van91I 2278 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1536 TCTAGA T^CTAGA XhoI 647 CTCGAG C^TCGAG XmnI 420 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 1993 CGCG CG^CG 2231 CGCG AccIII 2146 TCCGGA T^CCGGA 2948 TCCGGA AcyI 973 GACGCC G[GA]^CG[CT]C 2213 GACGCC AocI 1368 CCTAAGG CC^T[ACGT]AGG 2448 CCTGAGG AviII 9 TGCGCA TGC^GCA 2852 TGCGCA BalI 1008 TGGCCA TGG^CCA 2063 TGGCCA BlpI 2508 GCTCAGC GC^T[ACGT]AGC 2740 GCTAAGC Bpu1102I 2508 GCTCAGC GC^T[ACGT]AGC 2740 GCTAAGC BsaHI 973 GACGCC G[GA]^CG[CT]C 2213 GACGCC BsaI 280 GAGACC GGTCTC(1/5) 1766 GAGACC BseAI 2146 TCCGGA T^CCGGA 2948 TCCGGA BsgI 1236 CTGCAC GTGCAG(16/14) 2090 GTGCAG BspEI 2146 TCCGGA T^CCGGA 2948 TCCGGA BssSI 880 CACGAG CACGAG(-5/-1) 1292 CACGAG BstUI 1993 CGCG CG^CG 2231 CGCG Bsu36I 1368 CCTAAGG CC^T[ACGT]AGG 2448 CCTGAGG CelII 2508 GCTCAGC GC^T[ACGT]AGC 2740 GCTAAGC EarI 563 CTCTTC CTCTTC(1/4) 2359 CTCTTC Eco81I 1368 CCTAAGG CC^T[ACGT]AGG 2448 CCTGAGG EcoNI 1672 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1773 CCTGCAAGAGG FauI 365 CCCGC CCCGC(4/6) 2229 CCCGC FspI 9 TGCGCA TGC^GCA 2852 TGCGCA Hin1I 973 GACGCC G[GA]^CG[CT]C 2213 GACGCC Ksp632I 563 CTCTTC CTCTTC(1/4) 2359 CTCTTC MluNI 1008 TGGCCA TGG^CCA 2063 TGGCCA MroI 2146 TCCGGA T^CCGGA 2948 TCCGGA MscI 1008 TGGCCA TGG^CCA 2063 TGGCCA MvnI 1993 CGCG CG^CG 2231 CGCG PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT] 1436 AGGTCCC SacI 931 GAGCTC GAGCT^C 2313 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII