(Circular) (Six-base) MAPSORT of: pFN21AE1437.seq Check: 9946 from: 1 to: 7738 pFN21AE1437 7738 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3101 3101 Size: 7738 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5548 278 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3348 3497 5032 5081 1937 Size: 1411 149 1535 49 4594 Fragments arranged by size: 4594 1535 1411 149 49 Acc65I G'GTAC_C Cuts at: 3176 5059 3176 Size: 1883 5855 AclI AA'CG_TT Cuts at: 1563 4620 5865 6238 1563 Size: 3057 1245 373 3063 Fragments arranged by size: 3063 3057 1245 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 5801 7185 7453 1632 Size: 954 3215 1384 268 1917 Fragments arranged by size: 3215 1917 1384 954 268 AflII C'TTAA_G Cuts at: 820 1017 3340 820 Size: 197 2323 5218 Fragments arranged by size: 5218 2323 197 AflIII A'CryG_T Cuts at: 1203 3792 6637 7614 1203 Size: 2589 2845 977 1327 Fragments arranged by size: 2845 2589 1327 977 AgeI A'CCGG_T Cuts at: 6569 6569 Size: 7738 AhdI GACnn_n'nnGTC Cuts at: 2292 6467 2292 Size: 4175 3563 AleI CACnn'nnGTG Cuts at: 1166 4076 4645 1166 Size: 2910 569 4259 Fragments arranged by size: 4259 2910 569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2423 2907 1184 Size: 1239 484 6015 Fragments arranged by size: 6015 1239 484 AlwNI CAG_nnn'CTG Cuts at: 3587 5481 7053 3587 Size: 1894 1572 4272 Fragments arranged by size: 4272 1894 1572 ApaI G_GGCC'C Cuts at: 2975 2975 Size: 7738 ApaLI G'TGCA_C Cuts at: 5795 6951 5795 Size: 1156 6582 ApoI r'AATT_y Cuts at: 1493 2141 2628 3525 4470 4541 4585 5047 5273 Size: 648 487 897 945 71 44 462 226 Cuts at: 5273 5425 7534 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 945 897 648 487 462 226 152 71 44 AseI AT'TA_AT Cuts at: 160 1032 4212 6292 160 Size: 872 3180 2080 1606 Fragments arranged by size: 3180 2080 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 AvaI C'yCGr_G Cuts at: 1870 1943 2652 5063 1870 Size: 73 709 2411 4545 Fragments arranged by size: 4545 2411 709 73 BamHI G'GATC_C Cuts at: 3369 5068 5435 3369 Size: 1699 367 5672 Fragments arranged by size: 5672 1699 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3176 3919 4060 5059 6514 Size: 325 217 627 1389 743 141 999 1455 Cuts at: 6514 618 Size: 1842 Fragments arranged by size: 1842 1455 1389 999 743 627 325 217 141 BanII G_rGCy'C Cuts at: 1391 2510 2940 2975 3979 4118 4322 5057 1391 Size: 1119 430 35 1004 139 204 735 4072 Fragments arranged by size: 4072 1119 1004 735 430 204 139 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6767 BbvCI CC'TCA_GC Cuts at: 3486 3486 Size: 7738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5929 5963 1903 Size: 34 3992 34 3678 Fragments arranged by size: 3992 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5630 6840 5630 Size: 1210 6528 BclI T'GATC_A Cuts at: 1548 4131 1548 Size: 2583 5155 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6349 136 Size: 107 122 71 631 605 4677 1525 Fragments arranged by size: 4677 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4494 7733 4494 Size: 3239 4499 BlpI GC'TnA_GC Cuts at: 4514 4746 5155 4514 Size: 232 409 7097 Fragments arranged by size: 7097 409 232 Bme1580I G_kGCm'C Cuts at: 1790 2975 5799 6955 1790 Size: 1185 2824 1156 2573 Fragments arranged by size: 2824 2573 1185 1156 BmgBI CAC'GTC Cuts at: 1318 3382 3793 1318 Size: 2064 411 5263 Fragments arranged by size: 5263 2064 411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5469 6427 442 Size: 416 53 83 399 24 4052 958 1753 Fragments arranged by size: 4052 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7738 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2924 3455 3830 4406 6398 1239 Size: 857 828 531 375 576 1992 2579 Fragments arranged by size: 2579 1992 857 828 576 531 375 Bpu10I CC'TnA_GC Cuts at: 3486 3486 Size: 7738 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2757 3578 3845 4911 5863 6728 7026 2757 Size: 821 267 1066 952 865 298 3469 Fragments arranged by size: 3469 1066 952 865 821 298 267 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 3765 6401 882 Size: 617 780 1486 2636 2219 Fragments arranged by size: 2636 2219 1486 780 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 5529 493 Size: 711 1620 2705 2702 Fragments arranged by size: 2705 2702 1620 711 BsaBI GATnn'nnATC Cuts at: 7732 7732 Size: 7738 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2979 4219 5545 5927 Size: 53 83 186 936 1446 1240 1326 382 Cuts at: 5927 6562 275 Size: 635 1451 Fragments arranged by size: 1451 1446 1326 1240 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3366 4151 4953 6171 6569 6843 6990 7401 3366 Size: 785 802 1218 398 274 147 411 3703 Fragments arranged by size: 3703 1218 802 785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3135 3165 4149 4179 1178 Size: 30 165 30 1732 30 984 30 4737 Fragments arranged by size: 4737 1732 984 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2472 2924 2927 2948 3065 3827 4170 Size: 840 446 452 3 21 117 762 343 Cuts at: 4170 4471 4749 1186 Size: 301 278 4175 Fragments arranged by size: 4175 840 762 452 446 343 301 278 117 21 3 BseYI C'CCAG_C Cuts at: 3445 3636 4111 6941 7437 3445 Size: 191 475 2830 496 3746 Fragments arranged by size: 3746 2830 496 475 191 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3226 4116 1163 Size: 573 1490 890 4785 Fragments arranged by size: 4785 1490 890 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5188 5949 6977 1540 Size: 275 186 3187 761 1028 2301 Fragments arranged by size: 3187 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2659 2838 2886 2940 4173 4322 5799 5884 6955 Size: 179 48 54 1233 149 1477 85 1071 Cuts at: 6955 2659 Size: 3442 Fragments arranged by size: 3442 1477 1233 1071 179 149 85 54 48 BsmI GAATG_Cn' Cuts at: 5249 5342 5249 Size: 93 7645 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 2659 2838 2886 2940 2975 3979 Size: 399 720 149 179 48 54 35 1004 Cuts at: 3979 4118 4173 4322 5057 5799 5884 6955 1391 Size: 139 55 149 735 742 85 1071 2174 Fragments arranged by size: 2174 1071 1004 742 735 720 399 179 149 149 139 85 55 54 48 35 BspEI T'CCGG_A Cuts at: 4151 4953 7401 4151 Size: 802 2448 4488 Fragments arranged by size: 4488 2448 802 BspHI T'CATG_A Cuts at: 4807 5627 7357 4807 Size: 820 1730 5188 Fragments arranged by size: 5188 1730 820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 5093 844 Size: 2257 1992 3489 Fragments arranged by size: 3489 2257 1992 BsrBI CCG'CTC Cuts at: 1394 1823 5625 1394 Size: 429 3802 3507 Fragments arranged by size: 3802 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6233 6407 65 Size: 1183 4985 174 1396 Fragments arranged by size: 4985 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6382 6569 1700 Size: 110 4572 187 2869 Fragments arranged by size: 4572 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7738 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7738 BssSI C'ACGA_G Cuts at: 2885 3297 5798 6810 2885 Size: 412 2501 1012 3813 Fragments arranged by size: 3813 2501 1012 412 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7738 BstBI TT'CG_AA Cuts at: 6542 6542 Size: 7738 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7738 BstXI CCAn_nnnn'nTGG Cuts at: 4606 4606 Size: 7738 BstYI r'GATC_y Cuts at: 1972 2610 3369 4494 5068 5435 5820 5837 7289 Size: 638 759 1125 574 367 385 17 1452 Cuts at: 7289 7375 7387 7733 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1125 759 638 574 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3498 3498 Size: 7738 Bsu36I CC'TnA_GG Cuts at: 3374 4454 3374 Size: 1080 6658 BtgI C'CryG_G Cuts at: 513 1065 2004 3997 513 Size: 552 939 1993 4254 Fragments arranged by size: 4254 1993 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3981 487 Size: 683 56 2755 4244 Fragments arranged by size: 4244 2755 683 56 BtsI GCAGTG_nn' Cuts at: 2407 5254 6048 6068 2407 Size: 2847 794 20 4077 Fragments arranged by size: 4077 2847 794 20 ClaI AT'CG_AT Cuts at: 1554 2866 1554 Size: 1312 6426 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7703 DraI TTT'AAA Cuts at: 3570 3594 4423 5042 3570 Size: 24 829 619 6266 Fragments arranged by size: 6266 829 619 24 DraIII CAC_nnn'GTG Cuts at: 2756 2756 Size: 7738 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3422 6745 809 Size: 507 2106 3323 1802 Fragments arranged by size: 3323 2106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3013 4068 4875 Size: 54 1425 50 275 18 1183 1055 807 Cuts at: 4875 5185 6074 7703 8 Size: 310 889 1629 43 Fragments arranged by size: 1629 1425 1183 1055 889 807 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5185 1537 Size: 275 3373 4090 Fragments arranged by size: 4090 3373 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2574 4370 5668 1353 Size: 1221 1796 1298 3423 Fragments arranged by size: 3423 1796 1298 1221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3728 6325 6699 6845 387 Size: 1064 138 2139 2597 374 146 1280 Fragments arranged by size: 2597 2139 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2938 4320 2938 Size: 1382 6356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2586 2924 3455 3830 4406 5801 Size: 393 464 490 338 531 375 576 1395 Cuts at: 5801 6398 7185 7453 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1395 787 597 576 531 490 464 393 375 338 268 EcoNI CCTnn'n_nnAGG Cuts at: 3681 3782 3681 Size: 101 7637 EcoO109I rG'GnC_Cy Cuts at: 2596 2972 3442 4126 4725 2596 Size: 376 470 684 599 5609 Fragments arranged by size: 5609 684 599 470 376 EcoRI G'AATT_C Cuts at: 4585 5047 5425 4585 Size: 462 378 6898 Fragments arranged by size: 6898 462 378 FspI TGC'GCA Cuts at: 2016 4859 6244 2016 Size: 2843 1385 3510 Fragments arranged by size: 3510 2843 1385 FspAI rTGC'GCAy Cuts at: 4859 4859 Size: 7738 HaeII r_GCGC'y Cuts at: 1678 3988 6885 1678 Size: 2310 2897 2531 Fragments arranged by size: 2897 2531 2310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2278 2310 3779 3811 4789 Size: 32 135 32 706 32 1469 32 978 Cuts at: 4789 4821 6379 6411 6453 6485 1373 Size: 32 1558 32 42 32 2626 Fragments arranged by size: 2626 1558 1469 978 706 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5033 5082 5328 669 Size: 988 281 3095 49 246 3079 Fragments arranged by size: 3095 3079 988 281 246 49 HindIII A'AGCT_T Cuts at: 748 2130 748 Size: 1382 6356 HpaI GTT'AAC Cuts at: 5328 5328 Size: 7738 KpnI G_GTAC'C Cuts at: 3180 5063 3180 Size: 1883 5855 MfeI C'AATT_G Cuts at: 5337 5337 Size: 7738 MluI A'CGCG_T Cuts at: 7614 7614 Size: 7738 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3861 4270 6852 7036 1305 Size: 2556 409 2582 184 2007 Fragments arranged by size: 2582 2556 2007 409 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3015 4070 10 Size: 54 1425 343 1183 1055 3678 Fragments arranged by size: 3678 1425 1183 1055 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2889 2990 4058 4076 4604 4645 Size: 648 1135 588 101 1068 18 528 41 Cuts at: 4645 4947 5696 6055 6214 518 Size: 302 749 359 159 2042 Fragments arranged by size: 2042 1135 1068 749 648 588 528 359 302 159 101 41 18 MspA1I CmG'CkG Cuts at: 1606 3999 5154 5831 6979 7224 1606 Size: 2393 1155 677 1148 245 2120 Fragments arranged by size: 2393 2120 1155 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6247 Fragments arranged by size: 6247 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7738 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7738 NotI GC'GGCC_GC Cuts at: 5185 5185 Size: 7738 NspI r_CATG'y Cuts at: 1132 1229 2788 5096 6641 1132 Size: 97 1559 2308 1545 2229 Fragments arranged by size: 2308 2229 1559 1545 97 PasI CC'CwG_GG Cuts at: 1348 2323 1348 Size: 975 6763 PciI A'CATG_T Cuts at: 6637 6637 Size: 7738 PflMI CCAn_nnn'nTGG Cuts at: 1108 4289 1108 Size: 3181 4557 PfoI T'CCnGG_A Cuts at: 2765 2940 3807 2765 Size: 175 867 6696 Fragments arranged by size: 6696 867 175 PmeI GTTT'AAAC Cuts at: 5042 5042 Size: 7738 PmlI CAC'GTG Cuts at: 2824 2824 Size: 7738 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4746 4778 5807 5839 7320 7352 4746 Size: 32 1029 32 1481 32 5132 Fragments arranged by size: 5132 1481 1029 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 3442 2596 Size: 846 6892 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7738 PsiI TTA'TAA Cuts at: 5308 5308 Size: 7738 PspOMI G'GGCC_C Cuts at: 2971 2971 Size: 7738 PstI C_TGCA'G Cuts at: 830 5090 830 Size: 4260 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7738 SacI G_AGCT'C Cuts at: 2940 4322 2940 Size: 1382 6356 SacII CC_GC'GG Cuts at: 4000 4000 Size: 7738 SalI G'TCGA_C Cuts at: 1936 5031 5080 1936 Size: 3095 49 4594 Fragments arranged by size: 4594 3095 49 SbfI CC_TGCA'GG Cuts at: 5090 5090 Size: 7738 ScaI AGT'ACT Cuts at: 5986 7698 5986 Size: 1712 6026 SfcI C'TryA_G Cuts at: 826 1045 3749 4729 4894 5086 6221 6902 7093 Size: 219 2704 980 165 192 1135 681 191 Cuts at: 7093 826 Size: 1471 Fragments arranged by size: 2704 1471 1135 980 681 219 192 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7738 SmaI CCC'GGG Cuts at: 1872 5065 1872 Size: 3193 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2652 2772 3340 3557 3860 4926 Size: 197 926 709 120 568 217 303 1066 Cuts at: 4926 5842 6743 7005 820 Size: 916 901 262 1553 Fragments arranged by size: 1553 1066 926 916 901 709 568 303 262 217 197 120 SnaBI TAC'GTA Cuts at: 493 5529 493 Size: 5036 2702 SpeI A'CTAG_T Cuts at: 152 3280 152 Size: 3128 4610 SphI G_CATG'C Cuts at: 1132 5096 1132 Size: 3964 3774 SspI AAT'ATT Cuts at: 5 52 2855 5662 5 Size: 47 2803 2807 2081 Fragments arranged by size: 2807 2803 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7738 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 5177 513 Size: 552 939 588 2585 3074 Fragments arranged by size: 3074 2585 939 588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2163 5765 5782 5935 6120 6565 325 Size: 1255 583 3602 17 153 185 445 1498 Fragments arranged by size: 3602 1498 1255 583 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5984 7696 96 Size: 274 80 33 51 1443 4007 1712 138 Fragments arranged by size: 4007 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3135 3167 4149 4181 7698 7730 3135 Size: 32 982 32 3517 32 3143 Fragments arranged by size: 3517 3143 982 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7738 XbaI T'CTAG_A Cuts at: 3541 5074 3541 Size: 1533 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 2228 2320 4438 2092 Size: 136 92 2118 5392 Fragments arranged by size: 5392 2118 136 92 XhoI C'TCGA_G Cuts at: 1943 2652 1943 Size: 709 7029 XmaI C'CCGG_G Cuts at: 1870 5063 1870 Size: 3193 4545 XmnI GAAnn'nnTTC Cuts at: 2429 5867 2429 Size: 3438 4300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5546 276 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI