################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbrPI BbvCI BcgI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AgeI 915 ACCGGT A^CCGGT ApaI 92 GGGCCC GGGCC^C AvrII 837 CCTAGG C^CTAGG BbsI 637 GAAGAC GAAGAC(2/6) BciVI 19 GGATAC GTATCC(6/5) BglII 1043 AGATCT A^GATCT BlnI 837 CCTAGG C^CTAGG BpuAI 637 GAAGAC GAAGAC(2/6) BsaWI 915 ACCGGT [AT]^CCGG[AT] BsgI 301 GTGCAG GTGCAG(16/14) BsmBI 965 CGTCTC CGTCTC(1/5) BspDI 760 ATCGAT AT^CGAT BspMI 1301 ACCTGC ACCTGC(4/8) BstYI 1043 AGATCT [GA]^GATC[CT] ClaI 760 ATCGAT AT^CGAT EarI 643 GAAGAG CTCTTC(1/4) HgaI 1468 GACGC GACGC(5/10) Ksp632I 643 GAAGAG CTCTTC(1/4) MaeII 152 ACGT A^CGT MaeIII 1416 GTTAC ^GT[ACGT]AC MflI 1043 AGATCT [GA]^GATC[CT] NaeI 545 GCCGGC GCC^GGC NgoMIV 545 GCCGGC G^CCGGC NlaIII 483 CATG CATG^ PflMI 1040 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 915 ACCGGT A^CCGGT SacI 694 GAGCTC GAGCT^C SexAI 526 ACCTGGT A^CC[AT]GGT SfaNI 306 GCATC GCATC(5/9) SfiI 189 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 544 CGCCGGCG C[GA]^CCGG[CT]G TaqI 761 TCGA T^CGA TthHB8I 761 TCGA T^CGA Van91I 1040 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1043 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 168 GTAC GT^AC 366 GTAC BalI 360 TGGCCA TGG^CCA 488 TGGCCA BfaI 676 CTAG C^TAG 838 CTAG BglI 190 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1443 GCCCAGGCGGC BpmI 932 CTCCAG CTGGAG(16/14) 682 CTGGAG BsaI 1112 GGTCTC GGTCTC(1/5) 1191 GGTCTC EcoNI 510 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 552 CCTCAAGAAGG HphI 497 TCACC GGTGA(8/7) 512 TCACC MaeI 676 CTAG C^TAG 838 CTAG MluNI 360 TGGCCA TGG^CCA 488 TGGCCA MscI 360 TGGCCA TGG^CCA 488 TGGCCA MslI 498 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 513 CACCCAGGTG NotI 187 GCGGCCGC GC^GGCCGC 196 GCGGCCGC PstI 1030 CTGCAG CTGCA^G 1147 CTGCAG PvuII 13 CAGCTG CAG^CTG 262 CAGCTG RsaI 168 GTAC GT^AC 366 GTAC SmlI 553 CTCAAG C^T[CT][GA]AG 1420 CTCAAG StuI 428 AGGCCT AGG^CCT 840 AGGCCT TspRI 594 CACTG CA[GC]TG[ACGT][ACGT]^ 947 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PleI PpuMI SacII Sau3AI Sau96I ScrFI SfcI SfoI SmaI StyI TseI XmaCI XmaI