(Circular) (Six-base) MAPSORT of: pFN21AB7764.seq Check: 5922 from: 1 to: 6208 pFN21AB7764 6208 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3315 3315 Size: 6208 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2450 4271 5655 5923 1632 Size: 677 141 1821 1384 268 1917 Fragments arranged by size: 1917 1821 1384 677 268 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 2920 5039 2920 Size: 2119 4089 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 2522 1166 Size: 1356 4852 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 3951 5523 3951 Size: 1572 4636 ApaI G_GGCC'C Cuts at: 2101 2101 Size: 6208 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2100 2140 2300 2702 3533 1870 Size: 73 119 38 40 160 402 831 4545 Fragments arranged by size: 4545 831 402 160 119 73 40 38 AvrII C'CTAG_G Cuts at: 2842 2842 Size: 6208 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2179 2225 2553 2584 Size: 325 217 627 327 65 46 328 31 Cuts at: 2584 3529 4984 618 Size: 945 1455 1842 Fragments arranged by size: 1842 1455 945 627 328 327 325 217 65 46 31 BanII G_rGCy'C Cuts at: 1391 2101 2703 3293 3375 3527 1391 Size: 710 602 590 82 152 4072 Fragments arranged by size: 4072 710 602 590 152 82 BbeI G_GCGC'C Cuts at: 2183 2229 2557 2588 2183 Size: 46 328 31 5803 Fragments arranged by size: 5803 328 46 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2649 928 Size: 971 750 4487 Fragments arranged by size: 4487 971 750 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2018 4100 5310 2018 Size: 2082 1210 2916 Fragments arranged by size: 2916 2082 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2201 3454 4819 Size: 107 122 71 631 605 529 1253 1365 Cuts at: 4819 136 Size: 1525 Fragments arranged by size: 1525 1365 1253 631 605 529 122 107 71 BglII A'GATC_T Cuts at: 3048 6203 3048 Size: 3155 3053 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2101 3450 4269 5425 1790 Size: 311 1349 819 1156 2573 Fragments arranged by size: 2573 1349 1156 819 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2952 2952 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 2922 4868 1239 Size: 1469 214 1946 2579 Fragments arranged by size: 2579 1946 1469 214 Bpu10I CC'TnA_GC Cuts at: 2802 2802 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3410 4333 5198 5496 2543 Size: 867 923 865 298 3255 Fragments arranged by size: 3255 923 867 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3123 3202 4871 882 Size: 617 1624 79 1669 2219 Fragments arranged by size: 2219 1669 1624 617 79 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2180 2226 2554 2585 Size: 53 83 186 936 647 46 328 31 Cuts at: 2585 3474 4015 4397 5032 275 Size: 889 541 382 635 1451 Fragments arranged by size: 1451 936 889 647 635 541 382 328 186 83 53 46 31 BsaWI w'CCGG_w Cuts at: 2920 4641 5039 5313 5460 5871 2920 Size: 1721 398 274 147 411 3257 Fragments arranged by size: 3257 1721 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3263 3293 3404 3434 1178 Size: 30 165 30 1860 30 111 30 3952 Fragments arranged by size: 3952 1860 165 111 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 2651 2666 2675 2705 2708 2711 2726 Size: 1435 30 15 9 30 3 3 15 Cuts at: 2726 2729 2774 3490 1186 Size: 3 45 716 3904 Fragments arranged by size: 3904 1435 716 45 30 30 15 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 2016 2020 2269 2816 3208 3286 3310 5411 5907 Size: 4 249 547 392 78 24 2101 496 Cuts at: 5907 2016 Size: 2317 Fragments arranged by size: 2317 2101 547 496 392 249 78 24 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 1163 Size: 573 591 5044 Fragments arranged by size: 5044 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 2205 2319 3138 3168 3239 Size: 275 186 195 9 114 819 30 71 Cuts at: 3239 3658 4419 5447 1540 Size: 419 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 419 275 195 186 114 71 30 9 BsiHKAI G_wGCw'C Cuts at: 2163 2420 2451 2703 4269 4354 5425 2163 Size: 257 31 252 1566 85 1071 2946 Fragments arranged by size: 2946 1566 1071 257 252 85 31 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2976 2976 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2163 2420 2451 2703 3293 3375 Size: 399 311 62 257 31 252 590 82 Cuts at: 3375 3450 3527 4269 4354 5425 1391 Size: 75 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 590 399 311 257 252 85 82 77 75 62 31 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3315 3563 844 Size: 2471 248 3489 Fragments arranged by size: 3489 2471 248 BsrBI CCG'CTC Cuts at: 1394 1823 3096 3185 3277 4095 1394 Size: 429 1273 89 92 818 3507 Fragments arranged by size: 3507 1273 818 429 92 89 BsrDI GCAATG_nn' Cuts at: 65 1248 4703 4877 65 Size: 1183 3455 174 1396 Fragments arranged by size: 3455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2550 2910 2920 3234 4852 5039 1700 Size: 110 740 360 10 314 1618 187 2869 Fragments arranged by size: 2869 1618 740 360 314 187 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 2274 2546 2825 1925 Size: 349 272 279 5308 Fragments arranged by size: 5308 349 279 272 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstYI r'GATC_y Cuts at: 1972 3048 3538 3905 4290 4307 5759 5845 5857 Size: 1076 490 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 490 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2190 2244 2319 2958 3327 513 Size: 552 1125 54 75 639 369 3394 Fragments arranged by size: 3394 1125 639 552 369 75 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 2766 1554 Size: 1212 4996 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2202 2316 Size: 54 1425 50 275 18 363 9 114 Cuts at: 2316 2365 2493 2790 2912 2955 3165 3236 3362 Size: 49 128 297 122 43 210 71 126 Cuts at: 3362 3655 4544 6173 8 Size: 293 889 1629 43 Fragments arranged by size: 1629 1425 889 363 297 293 275 210 128 126 122 114 71 54 50 49 43 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2193 2202 2316 3165 3236 3655 1537 Size: 275 381 9 114 849 71 419 4090 Fragments arranged by size: 4090 849 419 381 275 114 71 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 4138 1353 Size: 1290 1495 3423 Fragments arranged by size: 3423 1495 1290 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 3019 3136 3396 4795 5169 Size: 1064 138 438 992 117 260 1399 374 Cuts at: 5169 5315 387 Size: 146 1280 Fragments arranged by size: 1399 1280 1064 992 438 374 260 146 138 117 EcoICRI GAG'CTC Cuts at: 2701 2701 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 2450 2708 2922 4271 4868 5655 Size: 393 677 141 258 214 1349 597 787 Cuts at: 5655 5923 1239 Size: 268 1524 Fragments arranged by size: 1524 1349 787 677 597 393 268 258 214 141 EcoNI CCTnn'n_nnAGG Cuts at: 2519 2561 2519 Size: 42 6166 EcoO109I rG'GnC_Cy Cuts at: 2097 2145 2383 2734 2852 2097 Size: 48 238 351 118 5453 Fragments arranged by size: 5453 351 238 118 48 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2062 2183 2229 2557 2588 2945 3206 5355 Size: 384 121 46 328 31 357 261 2149 Cuts at: 5355 1678 Size: 2531 Fragments arranged by size: 2531 2149 384 357 328 261 121 46 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4849 4881 4923 4955 1373 Size: 32 135 32 3277 32 42 32 2626 Fragments arranged by size: 3277 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2179 2225 2553 2584 2179 Size: 46 328 31 5803 Fragments arranged by size: 5803 328 46 31 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 5322 5506 1305 Size: 873 3144 184 2007 Fragments arranged by size: 3144 2007 873 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2367 2495 10 Size: 54 1425 343 535 128 3723 Fragments arranged by size: 3723 1425 535 343 128 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 2522 4166 4525 4684 518 Size: 648 1341 15 1644 359 159 2042 Fragments arranged by size: 2042 1644 1341 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2020 2072 2082 2192 2198 2246 2269 2321 Size: 414 52 10 110 6 48 23 52 Cuts at: 2321 3134 3208 3329 3368 3624 4301 5449 5694 Size: 813 74 121 39 256 677 1148 245 Cuts at: 5694 1606 Size: 2120 Fragments arranged by size: 2120 1148 813 677 414 256 245 121 110 74 52 52 48 39 23 10 6 NaeI GCC'GGC Cuts at: 2552 2552 Size: 6208 NarI GG'CG_CC Cuts at: 2180 2226 2554 2585 2180 Size: 46 328 31 5803 Fragments arranged by size: 5803 328 46 31 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5656 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2550 2550 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 2193 2202 3655 2193 Size: 9 1453 4746 Fragments arranged by size: 4746 1453 9 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 3051 1108 Size: 1943 4265 PfoI T'CCnGG_A Cuts at: 3319 3319 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2145 2383 2852 2145 Size: 238 469 5501 Fragments arranged by size: 5501 469 238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 2097 2097 Size: 6208 PstI C_TGCA'G Cuts at: 830 3039 3156 3560 830 Size: 2209 117 404 3478 Fragments arranged by size: 3478 2209 404 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2020 2269 2020 Size: 249 5959 SacI G_AGCT'C Cuts at: 2703 2703 Size: 6208 SacII CC_GC'GG Cuts at: 2193 2247 2322 3330 2193 Size: 54 75 1008 5071 Fragments arranged by size: 5071 1008 75 54 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SanDI GG'GwC_CC Cuts at: 2145 2383 2852 2145 Size: 238 469 5501 Fragments arranged by size: 5501 469 238 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2531 2531 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 3035 3152 3394 3556 4691 5372 5563 Size: 219 1990 117 242 162 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1990 1471 1135 681 242 219 191 162 117 SfiI GGCCn_nnn'nGGCC Cuts at: 2201 2201 Size: 6208 SfoI GGC'GCC Cuts at: 2181 2227 2555 2586 2181 Size: 46 328 31 5803 Fragments arranged by size: 5803 328 46 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 2550 1700 Size: 850 5358 SmaI CCC'GGG Cuts at: 1872 2064 2102 2302 3535 1872 Size: 192 38 200 1233 4545 Fragments arranged by size: 4545 1233 200 192 38 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3425 4312 5213 5475 820 Size: 197 926 615 867 887 901 262 1553 Fragments arranged by size: 1553 926 901 887 867 615 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2435 2847 1517 Size: 918 412 4878 Fragments arranged by size: 4878 918 412 StyI C'CwwG_G Cuts at: 513 1065 2215 2472 2842 3402 3647 513 Size: 552 1150 257 370 560 245 3074 Fragments arranged by size: 3074 1150 560 552 370 257 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 2534 4235 4252 4405 4590 5035 Size: 1255 522 432 1701 17 153 185 445 Cuts at: 5035 325 Size: 1498 Fragments arranged by size: 1701 1498 1255 522 445 432 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4454 6166 96 Size: 274 80 33 51 1443 2477 1712 138 Fragments arranged by size: 2477 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2907 2939 3263 3295 6168 6200 2907 Size: 32 324 32 2873 32 2915 Fragments arranged by size: 2915 2873 324 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2062 2100 2300 3533 1870 Size: 192 38 200 1233 4545 Fragments arranged by size: 4545 1233 200 192 38 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SrfI SwaI XcmI