(Linear) (Six-base) MAPSORT of: KIBB7764.seq Check: 5474 from: 1 to: 1503 KIBB7764.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1310 1503 Size: 1310 193 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 445 1503 Size: 304 141 1058 Fragments arranged by size: 1058 304 141 AgeI A'CCGG_T Cuts at: 0 915 1503 Size: 915 588 AleI CACnn'nnGTG Cuts at: 0 517 1503 Size: 517 986 ApaI G_GGCC'C Cuts at: 0 96 1503 Size: 96 1407 AvaI C'yCGr_G Cuts at: 0 57 95 135 295 697 1503 Size: 57 38 40 160 402 806 Fragments arranged by size: 806 402 160 57 40 38 AvrII C'CTAG_G Cuts at: 0 837 1503 Size: 837 666 BanI G'GyrC_C Cuts at: 0 109 174 220 548 579 1503 Size: 109 65 46 328 31 924 Fragments arranged by size: 924 328 109 65 46 31 BanII G_rGCy'C Cuts at: 0 96 698 1288 1370 1503 Size: 96 602 590 82 133 Fragments arranged by size: 602 590 133 96 82 BbeI G_GCGC'C Cuts at: 0 178 224 552 583 1503 Size: 178 46 328 31 920 Fragments arranged by size: 920 328 178 46 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 644 1503 Size: 644 859 BciVI GTATCCnnnnn_n' Cuts at: 0 13 1503 Size: 13 1490 BglI GCCn_nnn'nGGC Cuts at: 0 196 1449 1503 Size: 196 1253 54 Fragments arranged by size: 1253 196 54 BglII A'GATC_T Cuts at: 0 1043 1503 Size: 1043 460 Bme1580I G_kGCm'C Cuts at: 0 96 1445 1503 Size: 96 1349 58 Fragments arranged by size: 1349 96 58 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 947 1503 Size: 947 556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 917 1503 Size: 703 214 586 Fragments arranged by size: 703 586 214 Bpu10I CC'TnA_GC Cuts at: 0 797 1503 Size: 797 706 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 1405 1503 Size: 538 867 98 Fragments arranged by size: 867 538 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 1118 1197 1503 Size: 1118 79 306 Fragments arranged by size: 1118 306 79 BsaHI Gr'CG_yC Cuts at: 0 175 221 549 580 1469 1503 Size: 175 46 328 31 889 34 Fragments arranged by size: 889 328 175 46 34 31 BsaWI w'CCGG_w Cuts at: 0 915 1503 Size: 915 588 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1258 1288 1399 1429 1503 Size: 1258 30 111 30 74 Fragments arranged by size: 1258 111 74 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 616 646 661 670 700 703 706 721 Size: 616 30 15 9 30 3 3 15 Cuts at: 721 724 769 1485 1503 Size: 3 45 716 18 Fragments arranged by size: 716 616 45 30 30 18 15 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 11 15 264 811 1203 1281 1305 1503 Size: 11 4 249 547 392 78 24 198 Fragments arranged by size: 547 392 249 198 78 24 11 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1503 Size: 322 1181 BsiEI CG_ry'CG Cuts at: 0 191 200 314 1133 1163 1234 1503 Size: 191 9 114 819 30 71 269 Fragments arranged by size: 819 269 191 114 71 30 9 BsiHKAI G_wGCw'C Cuts at: 0 158 415 446 698 1503 Size: 158 257 31 252 805 Fragments arranged by size: 805 257 252 158 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 971 1503 Size: 971 532 Bsp1286I G_dGCh'C Cuts at: 0 96 158 415 446 698 1288 1370 1445 Size: 96 62 257 31 252 590 82 75 Cuts at: 1445 1503 Size: 58 Fragments arranged by size: 590 257 252 96 82 75 62 58 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1310 1503 Size: 1310 193 BsrBI CCG'CTC Cuts at: 0 1091 1180 1272 1503 Size: 1091 89 92 231 Fragments arranged by size: 1091 231 92 89 BsrFI r'CCGG_y Cuts at: 0 545 905 915 1229 1503 Size: 545 360 10 314 274 Fragments arranged by size: 545 360 314 274 10 BssHII G'CGCG_C Cuts at: 0 269 541 820 1503 Size: 269 272 279 683 Fragments arranged by size: 683 279 272 269 BstYI r'GATC_y Cuts at: 0 1043 1503 Size: 1043 460 BtgI C'CryG_G Cuts at: 0 185 239 314 953 1322 1503 Size: 185 54 75 639 369 181 Fragments arranged by size: 639 369 185 181 75 54 ClaI AT'CG_AT Cuts at: 0 761 1503 Size: 761 742 EaeI y'GGCC_r Cuts at: 0 188 197 311 360 488 785 907 950 Size: 188 9 114 49 128 297 122 43 Cuts at: 950 1160 1231 1357 1503 Size: 210 71 126 146 Fragments arranged by size: 297 210 188 146 128 126 122 114 71 49 43 9 EagI C'GGCC_G Cuts at: 0 188 197 311 1160 1231 1503 Size: 188 9 114 849 71 272 Fragments arranged by size: 849 272 188 114 71 9 EarI CTCTTCn'nnn_ Cuts at: 0 638 1503 Size: 638 865 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 1014 1131 1391 1503 Size: 22 992 117 260 112 Fragments arranged by size: 992 260 117 112 22 EcoICRI GAG'CTC Cuts at: 0 696 1503 Size: 696 807 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 445 703 917 1503 Size: 304 141 258 214 586 Fragments arranged by size: 586 304 258 214 141 EcoNI CCTnn'n_nnAGG Cuts at: 0 514 556 1503 Size: 514 42 947 Fragments arranged by size: 947 514 42 EcoO109I rG'GnC_Cy Cuts at: 0 92 140 378 729 847 1503 Size: 92 48 238 351 118 656 Fragments arranged by size: 656 351 238 118 92 48 HaeII r_GCGC'y Cuts at: 0 57 178 224 552 583 940 1201 1503 Size: 57 121 46 328 31 357 261 302 Fragments arranged by size: 357 328 302 261 121 57 46 31 KasI G'GCGC_C Cuts at: 0 174 220 548 579 1503 Size: 174 46 328 31 924 Fragments arranged by size: 924 328 174 46 31 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 173 1503 Size: 173 1330 MscI TGG'CCA Cuts at: 0 362 490 1503 Size: 362 128 1013 Fragments arranged by size: 1013 362 128 MslI CAynn'nnrTG Cuts at: 0 502 517 1503 Size: 502 15 986 Fragments arranged by size: 986 502 15 MspA1I CmG'CkG Cuts at: 0 15 67 77 187 193 241 264 316 Size: 15 52 10 110 6 48 23 52 Cuts at: 316 1129 1203 1324 1363 1503 Size: 813 74 121 39 140 Fragments arranged by size: 813 140 121 110 74 52 52 48 39 23 15 10 6 NaeI GCC'GGC Cuts at: 0 547 1503 Size: 547 956 NarI GG'CG_CC Cuts at: 0 175 221 549 580 1503 Size: 175 46 328 31 923 Fragments arranged by size: 923 328 175 46 31 NgoMIV G'CCGG_C Cuts at: 0 545 1503 Size: 545 958 NotI GC'GGCC_GC Cuts at: 0 188 197 1503 Size: 188 9 1306 Fragments arranged by size: 1306 188 9 PflMI CCAn_nnn'nTGG Cuts at: 0 1046 1503 Size: 1046 457 PfoI T'CCnGG_A Cuts at: 0 1314 1503 Size: 1314 189 PpuMI rG'GwC_Cy Cuts at: 0 140 378 847 1503 Size: 140 238 469 656 Fragments arranged by size: 656 469 238 140 PspOMI G'GGCC_C Cuts at: 0 92 1503 Size: 92 1411 PstI C_TGCA'G Cuts at: 0 1034 1151 1503 Size: 1034 117 352 Fragments arranged by size: 1034 352 117 PvuII CAG'CTG Cuts at: 0 15 264 1503 Size: 15 249 1239 Fragments arranged by size: 1239 249 15 SacI G_AGCT'C Cuts at: 0 698 1503 Size: 698 805 SacII CC_GC'GG Cuts at: 0 188 242 317 1325 1503 Size: 188 54 75 1008 178 Fragments arranged by size: 1008 188 178 75 54 SanDI GG'GwC_CC Cuts at: 0 140 378 847 1503 Size: 140 238 469 656 Fragments arranged by size: 656 469 238 140 SexAI A'CCwGG_T Cuts at: 0 526 1503 Size: 526 977 SfcI C'TryA_G Cuts at: 0 1030 1147 1389 1503 Size: 1030 117 242 114 Fragments arranged by size: 1030 242 117 114 SfiI GGCCn_nnn'nGGCC Cuts at: 0 196 1503 Size: 196 1307 SfoI GGC'GCC Cuts at: 0 176 222 550 581 1503 Size: 176 46 328 31 922 Fragments arranged by size: 922 328 176 46 31 SgrAI Cr'CCGG_yG Cuts at: 0 545 1503 Size: 545 958 SmaI CCC'GGG Cuts at: 0 59 97 297 1503 Size: 59 38 200 1206 Fragments arranged by size: 1206 200 59 38 SmlI C'TyrA_G Cuts at: 0 553 1420 1503 Size: 553 867 83 Fragments arranged by size: 867 553 83 StuI AGG'CCT Cuts at: 0 430 842 1503 Size: 430 412 661 Fragments arranged by size: 661 430 412 StyI C'CwwG_G Cuts at: 0 210 467 837 1397 1503 Size: 210 257 370 560 106 Fragments arranged by size: 560 370 257 210 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 97 529 1503 Size: 97 432 974 Fragments arranged by size: 974 432 97 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 902 934 1258 1290 1503 Size: 902 32 324 32 213 Fragments arranged by size: 902 324 213 32 32 XmaI C'CCGG_G Cuts at: 0 57 95 295 1503 Size: 57 38 200 1208 Fragments arranged by size: 1208 200 57 38 Enzymes that do cut: AarI AcuI AgeI AleI ApaI AvaI AvrII BanI BanII BbeI BbsI BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstYI BtgI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BclI BfrBI BlpI BmgBI BmrI BmtI BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI