(Circular) (Six-base) MAPSORT of: pFN21AE4466.seq Check: 6664 from: 1 to: 6169 pFN21AE4466 6169 bp With 182 enzymes: SgfI * March 28, 2012 14:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2646 3979 278 Size: 53 83 186 936 1110 1333 2468 Fragments arranged by size: 2468 1333 1110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2233 2399 2996 4232 5616 5884 1632 Size: 601 166 597 1236 1384 268 1917 Fragments arranged by size: 1917 1384 1236 601 597 268 166 AfeI AGC'GCT Cuts at: 2831 2831 Size: 6169 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 5068 6045 1203 Size: 3865 977 1327 Fragments arranged by size: 3865 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 2576 1166 Size: 1410 4759 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2546 3912 5484 2546 Size: 1366 1572 3231 Fragments arranged by size: 3231 1572 1366 ApaI G_GGCC'C Cuts at: 2117 2117 Size: 6169 ApaLI G'TGCA_C Cuts at: 3146 4226 5382 3146 Size: 1080 1156 3933 Fragments arranged by size: 3933 1156 1080 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2053 3494 1870 Size: 73 110 1441 4545 Fragments arranged by size: 4545 1441 110 73 BamHI G'GATC_C Cuts at: 2383 2847 3499 3866 2383 Size: 464 652 367 4686 Fragments arranged by size: 4686 652 464 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2205 2474 2655 3490 Size: 325 217 627 241 177 269 181 835 Cuts at: 3490 4945 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 835 627 325 269 241 217 181 177 BanII G_rGCy'C Cuts at: 1391 2117 2585 2799 3060 3488 1391 Size: 726 468 214 261 428 4072 Fragments arranged by size: 4072 726 468 428 261 214 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 6169 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5198 BbvCI CC'TCA_GC Cuts at: 2201 2201 Size: 6169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2064 2519 3458 Size: 107 122 71 631 605 392 455 939 Cuts at: 3458 4780 136 Size: 1322 1525 Fragments arranged by size: 1525 1322 939 631 605 455 392 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 3054 3586 3054 Size: 532 5637 Bme1580I G_kGCm'C Cuts at: 1790 2117 2479 3150 4230 5386 1790 Size: 327 362 671 1080 1156 2573 Fragments arranged by size: 2573 1156 1080 671 362 327 BmgBI CAC'GTC Cuts at: 1318 2122 1318 Size: 804 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2203 3900 4858 Size: 416 53 83 399 24 786 1697 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1753 1697 958 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3285 3285 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2559 2861 4829 1239 Size: 1100 220 302 1968 2579 Fragments arranged by size: 2579 1968 1100 302 220 Bpu10I CC'TnA_GC Cuts at: 2201 2201 Size: 6169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2317 2606 2873 4294 5159 5457 2317 Size: 289 267 1421 865 298 3029 Fragments arranged by size: 3029 1421 865 298 289 267 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3066 4832 882 Size: 617 1567 1766 2219 Fragments arranged by size: 2219 1766 1567 617 BsaAI yAC'GTr Cuts at: 493 1204 2509 3436 3960 493 Size: 711 1305 927 524 2702 Fragments arranged by size: 2702 1305 927 711 524 BsaBI GATnn'nnATC Cuts at: 2291 6163 2291 Size: 3872 2297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2103 2172 2643 Size: 53 83 186 936 496 74 69 471 Cuts at: 2643 3976 4358 4993 275 Size: 1333 382 635 1451 Fragments arranged by size: 1451 1333 936 635 496 471 382 186 83 74 69 53 BsaWI w'CCGG_w Cuts at: 2823 4602 5000 5274 5421 5832 2823 Size: 1779 398 274 147 411 3160 Fragments arranged by size: 3160 1779 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 1178 Size: 30 165 30 786 30 5128 Fragments arranged by size: 5128 786 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 1186 Size: 919 5250 BseYI C'CCAG_C Cuts at: 2539 2871 3454 5372 5868 2539 Size: 332 583 1918 496 2840 Fragments arranged by size: 2840 1918 583 496 332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 1163 Size: 573 753 4843 Fragments arranged by size: 4843 753 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 3619 4380 5408 1540 Size: 275 186 13 1605 761 1028 2301 Fragments arranged by size: 2301 1605 1028 761 275 186 13 BsiHKAI G_wGCw'C Cuts at: 2799 3060 3150 3441 4230 4315 5386 2799 Size: 261 90 291 789 85 1071 3582 Fragments arranged by size: 3582 1071 789 291 261 90 85 BsmI GAATG_Cn' Cuts at: 2444 3049 3680 3773 2444 Size: 605 631 93 4840 Fragments arranged by size: 4840 631 605 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2163 2163 Size: 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2479 2585 2799 3060 3150 3441 Size: 399 327 362 106 214 261 90 291 Cuts at: 3441 3488 4230 4315 5386 1391 Size: 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 362 327 291 261 214 106 90 85 47 BspEI T'CCGG_A Cuts at: 2823 5832 2823 Size: 3009 3160 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 844 Size: 2680 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2811 4056 1394 Size: 429 988 1245 3507 Fragments arranged by size: 3507 1245 988 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2308 4664 4838 65 Size: 1183 1060 2356 174 1396 Fragments arranged by size: 2356 1396 1183 1060 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2174 4813 5000 1700 Size: 110 364 2639 187 2869 Fragments arranged by size: 2869 2639 364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 3248 4229 5241 3248 Size: 981 1012 4176 Fragments arranged by size: 4176 1012 981 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6169 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2362 3327 2362 Size: 965 5204 BstYI r'GATC_y Cuts at: 1972 2383 2847 3315 3499 3866 4251 4268 5720 Size: 411 464 468 184 367 385 17 1452 Cuts at: 5720 5806 5818 6164 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 468 464 411 385 367 346 184 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2014 2059 2130 2260 3048 513 Size: 552 939 10 45 71 130 788 3634 Fragments arranged by size: 3634 939 788 552 130 71 45 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2195 487 Size: 683 56 969 4461 Fragments arranged by size: 4461 969 683 56 BtsI GCAGTG_nn' Cuts at: 2710 2903 3685 4479 4499 2710 Size: 193 782 794 20 4380 Fragments arranged by size: 4380 794 782 193 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2242 2277 529 Size: 35 1678 35 4421 Fragments arranged by size: 4421 1678 35 35 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 2725 2787 2725 Size: 62 6107 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2188 2421 2511 Size: 54 1425 50 275 18 358 233 90 Cuts at: 2511 2520 3210 3616 4505 6134 8 Size: 9 690 406 889 1629 43 Fragments arranged by size: 1629 1425 889 690 406 358 275 233 90 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2621 2633 2777 3032 4099 1353 Size: 729 539 12 144 255 1067 3423 Fragments arranged by size: 3423 1067 729 539 255 144 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 3272 4756 5130 5276 387 Size: 1064 138 510 1173 1484 374 146 1280 Fragments arranged by size: 1484 1280 1173 1064 510 374 146 138 EcoICRI GAG'CTC Cuts at: 2797 3058 2797 Size: 261 5908 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2233 2339 2399 2559 2861 2996 4232 Size: 393 601 106 60 160 302 135 1236 Cuts at: 4232 4829 5616 5884 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1236 787 601 597 393 302 268 160 135 106 60 EcoO109I rG'GnC_Cy Cuts at: 2114 3451 2114 Size: 1337 4832 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 2032 2464 2597 2833 2971 5316 1678 Size: 354 432 133 236 138 2345 2531 Fragments arranged by size: 2531 2345 432 354 236 138 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2226 2633 2665 4810 Size: 32 135 32 622 32 407 32 2145 Cuts at: 4810 4842 4884 4916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2145 622 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3513 3759 669 Size: 988 281 709 866 246 3079 Fragments arranged by size: 3079 988 866 709 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KasI G'GCGC_C Cuts at: 2028 2028 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5283 5467 1305 Size: 3978 184 2007 Fragments arranged by size: 3978 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 2522 3212 10 Size: 54 1425 343 681 9 690 2967 Fragments arranged by size: 2967 1425 690 681 343 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2345 2360 2576 3143 3302 4127 4486 Size: 648 1179 15 216 567 159 825 359 Cuts at: 4486 4645 518 Size: 159 2042 Fragments arranged by size: 2042 1179 825 648 567 359 216 159 159 15 MspA1I CmG'CkG Cuts at: 1606 2016 2108 2132 2187 2543 3371 3458 3585 Size: 410 92 24 55 356 828 87 127 Cuts at: 3585 4262 5410 5655 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 828 677 410 356 245 127 92 87 55 24 NaeI GCC'GGC Cuts at: 2176 2176 Size: 6169 NarI GG'CG_CC Cuts at: 2029 2029 Size: 6169 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4678 Fragments arranged by size: 4678 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NgoMIV G'CCGG_C Cuts at: 2174 2174 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PmlI CAC'GTG Cuts at: 2509 2509 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2846 2878 4238 4270 5751 5783 2846 Size: 32 1360 32 1481 32 3232 Fragments arranged by size: 3232 1481 1360 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2113 2113 Size: 6169 PspXI vC'TCGA_Gb Cuts at: 2053 2053 Size: 6169 PstI C_TGCA'G Cuts at: 830 2220 2689 3521 830 Size: 1390 469 832 3478 Fragments arranged by size: 3478 1390 832 469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 3371 3371 Size: 6169 SacI G_AGCT'C Cuts at: 2799 3060 2799 Size: 261 5908 SacII CC_GC'GG Cuts at: 2017 2133 2017 Size: 116 6053 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SapI GCTCTTCn'nnn_ Cuts at: 2633 3032 2633 Size: 399 5770 SbfI CC_TGCA'GG Cuts at: 2220 3521 2220 Size: 1301 4868 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SexAI A'CCwGG_T Cuts at: 3255 3255 Size: 6169 SfcI C'TryA_G Cuts at: 826 1045 2216 2389 2685 3517 4652 5333 5524 Size: 219 1171 173 296 832 1135 681 191 Cuts at: 5524 826 Size: 1471 Fragments arranged by size: 1471 1171 1135 832 681 296 219 191 173 SfiI GGCCn_nnn'nGGCC Cuts at: 2519 2519 Size: 6169 SfoI GGC'GCC Cuts at: 2030 2030 Size: 6169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 2174 1700 Size: 474 5695 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2296 2621 2852 4273 5174 Size: 197 926 110 243 325 231 1421 901 Cuts at: 5174 5436 820 Size: 262 1553 Fragments arranged by size: 1553 1421 926 901 325 262 243 231 197 110 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 2673 3608 513 Size: 552 939 669 935 3074 Fragments arranged by size: 3074 939 935 669 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 2885 4196 4213 4366 4551 4996 Size: 1255 882 423 1311 17 153 185 445 Cuts at: 4996 325 Size: 1498 Fragments arranged by size: 1498 1311 1255 882 445 423 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4415 6127 96 Size: 274 80 33 51 1443 2438 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 6129 6161 2189 Size: 32 3908 32 2197 Fragments arranged by size: 3908 2197 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2041 1316 Size: 725 5444 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3356 3356 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 2053 1943 Size: 110 6059 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 2933 4298 2933 Size: 1365 4804 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2644 3977 276 Size: 53 83 186 936 1110 1333 2468 Fragments arranged by size: 2468 1333 1110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PsrI RsrII SanDI SrfI SwaI