(Linear) (Six-base) MAPSORT of: KIBB5793.seq Check: 6802 from: 1 to: 2493 KIBB5793.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AccI GT'mk_AC Cuts at: 0 88 1238 2493 Size: 88 1150 1255 Fragments arranged by size: 1255 1150 88 Acc65I G'GTAC_C Cuts at: 0 2141 2493 Size: 2141 352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 851 1703 1901 2101 2493 Size: 576 275 852 198 200 392 Fragments arranged by size: 852 576 392 275 200 198 AflIII A'CryG_T Cuts at: 0 1679 2493 Size: 1679 814 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2408 2493 Size: 2408 85 AlwNI CAG_nnn'CTG Cuts at: 0 78 1413 1726 1879 1926 2493 Size: 78 1335 313 153 47 567 Fragments arranged by size: 1335 567 313 153 78 47 ApoI r'AATT_y Cuts at: 0 505 615 675 729 2150 2250 2262 2493 Size: 505 110 60 54 1421 100 12 231 Fragments arranged by size: 1421 505 231 110 100 60 54 12 BamHI G'GATC_C Cuts at: 0 977 2493 Size: 977 1516 BanI G'GyrC_C Cuts at: 0 2141 2493 Size: 2141 352 BanII G_rGCy'C Cuts at: 0 849 2493 Size: 849 1644 BbvCI CC'TCA_GC Cuts at: 0 94 2493 Size: 94 2399 BciVI GTATCCnnnnn_n' Cuts at: 0 345 957 2493 Size: 345 612 1536 Fragments arranged by size: 1536 612 345 BclI T'GATC_A Cuts at: 0 1283 2493 Size: 1283 1210 BfrBI ATG'CAT Cuts at: 0 1089 2493 Size: 1089 1404 BglI GCCn_nnn'nGGC Cuts at: 0 286 2493 Size: 286 2207 BglII A'GATC_T Cuts at: 0 474 630 2493 Size: 474 156 1863 Fragments arranged by size: 1863 474 156 BmrI ACTGGGnnnn_n' Cuts at: 0 371 1121 2493 Size: 371 750 1372 Fragments arranged by size: 1372 750 371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 587 1124 1610 1748 2029 2116 2493 Size: 587 537 486 138 281 87 377 Fragments arranged by size: 587 537 486 377 281 138 87 Bpu10I CC'TnA_GC Cuts at: 0 94 2493 Size: 94 2399 BsaI GGTCTCn'nnnn_ Cuts at: 0 1797 2493 Size: 1797 696 BsaHI Gr'CG_yC Cuts at: 0 225 1235 2493 Size: 225 1010 1258 Fragments arranged by size: 1258 1010 225 BsaWI w'CCGG_w Cuts at: 0 2144 2238 2493 Size: 2144 94 255 Fragments arranged by size: 2144 255 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1207 1237 1387 1417 2422 2452 2493 Size: 1207 30 150 30 1005 30 41 Fragments arranged by size: 1207 1005 150 41 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 52 55 265 2493 Size: 49 3 3 210 2228 Fragments arranged by size: 2228 210 49 3 3 BseYI C'CCAG_C Cuts at: 0 170 1019 2493 Size: 170 849 1474 Fragments arranged by size: 1474 849 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1392 2493 Size: 1392 1101 BsiEI CG_ry'CG Cuts at: 0 188 219 261 2493 Size: 188 31 42 2232 Fragments arranged by size: 2232 188 42 31 BsiHKAI G_wGCw'C Cuts at: 0 1256 1616 2327 2493 Size: 1256 360 711 166 Fragments arranged by size: 1256 711 360 166 BsmI GAATG_Cn' Cuts at: 0 1452 2493 Size: 1452 1041 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1195 2493 Size: 1195 1298 Bsp1286I G_dGCh'C Cuts at: 0 849 1256 1616 2327 2493 Size: 849 407 360 711 166 Fragments arranged by size: 849 711 407 360 166 BspEI T'CCGG_A Cuts at: 0 2238 2493 Size: 2238 255 BspHI T'CATG_A Cuts at: 0 2463 2493 Size: 2463 30 BsrBI CCG'CTC Cuts at: 0 6 2493 Size: 6 2487 BsrDI GCAATG_nn' Cuts at: 0 1463 2493 Size: 1463 1030 BsrFI r'CCGG_y Cuts at: 0 104 2493 Size: 104 2389 BsrGI T'GTAC_A Cuts at: 0 1514 2493 Size: 1514 979 BssHII G'CGCG_C Cuts at: 0 233 2493 Size: 233 2260 BstAPI GCAn_nnn'nTGC Cuts at: 0 78 2493 Size: 78 2415 BstXI CCAn_nnnn'nTGG Cuts at: 0 1167 1860 2064 2409 2493 Size: 1167 693 204 345 84 Fragments arranged by size: 1167 693 345 204 84 BstYI r'GATC_y Cuts at: 0 474 630 977 2493 Size: 474 156 347 1516 Fragments arranged by size: 1516 474 347 156 Bsu36I CC'TnA_GG Cuts at: 0 1318 2493 Size: 1318 1175 BtgI C'CryG_G Cuts at: 0 45 210 1031 1230 2493 Size: 45 165 821 199 1263 Fragments arranged by size: 1263 821 199 165 45 ClaI AT'CG_AT Cuts at: 0 2370 2493 Size: 2370 123 DraI TTT'AAA Cuts at: 0 1812 2493 Size: 1812 681 EaeI y'GGCC_r Cuts at: 0 185 216 258 287 773 1604 2282 2493 Size: 185 31 42 29 486 831 678 211 Fragments arranged by size: 831 678 486 211 185 42 31 29 EagI C'GGCC_G Cuts at: 0 185 216 258 2493 Size: 185 31 42 2235 Fragments arranged by size: 2235 185 42 31 EarI CTCTTCn'nnn_ Cuts at: 0 1832 2109 2493 Size: 1832 277 384 Fragments arranged by size: 1832 384 277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1211 2493 Size: 1211 1282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 587 851 1124 1610 1703 1748 1901 Size: 576 11 264 273 486 93 45 153 Cuts at: 1901 2029 2101 2116 2493 Size: 128 72 15 377 Fragments arranged by size: 576 486 377 273 264 153 128 93 72 45 15 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 97 2493 Size: 97 2396 EcoO109I rG'GnC_Cy Cuts at: 0 1561 1692 2481 2493 Size: 1561 131 789 12 Fragments arranged by size: 1561 789 131 12 EcoRI G'AATT_C Cuts at: 0 675 2250 2493 Size: 675 1575 243 Fragments arranged by size: 1575 675 243 EcoRV GAT'ATC Cuts at: 0 1818 2493 Size: 1818 675 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 856 1837 2493 Size: 856 981 656 Fragments arranged by size: 981 856 656 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 572 604 1136 1168 1477 1509 Size: 16 32 524 32 532 32 309 32 Cuts at: 1509 2422 2454 2493 Size: 913 32 39 Fragments arranged by size: 913 532 524 309 39 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 0 89 551 2493 Size: 89 462 1942 Fragments arranged by size: 1942 462 89 HindIII A'AGCT_T Cuts at: 0 882 2493 Size: 882 1611 HpaI GTT'AAC Cuts at: 0 551 2493 Size: 551 1942 KpnI G_GTAC'C Cuts at: 0 2145 2493 Size: 2145 348 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 330 2493 Size: 330 2163 MscI TGG'CCA Cuts at: 0 289 775 1606 2284 2493 Size: 289 486 831 678 209 Fragments arranged by size: 831 678 486 289 209 MslI CAynn'nnrTG Cuts at: 0 163 305 655 1094 1385 1495 1600 1947 Size: 163 142 350 439 291 110 105 347 Cuts at: 1947 2493 Size: 546 Fragments arranged by size: 546 439 350 347 291 163 142 110 105 MspA1I CmG'CkG Cuts at: 0 75 130 138 212 1232 2493 Size: 75 55 8 74 1020 1261 Fragments arranged by size: 1261 1020 75 74 55 8 NcoI C'CATG_G Cuts at: 0 45 2493 Size: 45 2448 NdeI CA'TA_TG Cuts at: 0 1855 2493 Size: 1855 638 NotI GC'GGCC_GC Cuts at: 0 185 2493 Size: 185 2308 NsiI A_TGCA'T Cuts at: 0 1091 2493 Size: 1091 1402 NspI r_CATG'y Cuts at: 0 310 1093 2395 2493 Size: 310 783 1302 98 Fragments arranged by size: 1302 783 310 98 PasI CC'CwG_GG Cuts at: 0 11 2493 Size: 11 2482 PflMI CCAn_nnn'nTGG Cuts at: 0 1787 2094 2493 Size: 1787 307 399 Fragments arranged by size: 1787 399 307 PfoI T'CCnGG_A Cuts at: 0 266 1540 1580 2493 Size: 266 1274 40 913 Fragments arranged by size: 1274 913 266 40 PpuMI rG'GwC_Cy Cuts at: 0 1561 1692 2481 2493 Size: 1561 131 789 12 Fragments arranged by size: 1561 789 131 12 PshAI GACnn'nnGTC Cuts at: 0 1658 2493 Size: 1658 835 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2171 2203 2493 Size: 2171 32 290 Fragments arranged by size: 2171 290 32 PstI C_TGCA'G Cuts at: 0 74 2387 2493 Size: 74 2313 106 Fragments arranged by size: 2313 106 74 PvuII CAG'CTG Cuts at: 0 75 2493 Size: 75 2418 SacII CC_GC'GG Cuts at: 0 213 1233 2493 Size: 213 1020 1260 Fragments arranged by size: 1260 1020 213 SalI G'TCGA_C Cuts at: 0 87 2493 Size: 87 2406 SanDI GG'GwC_CC Cuts at: 0 1692 2493 Size: 1692 801 SfcI C'TryA_G Cuts at: 0 70 1349 1395 2383 2493 Size: 70 1279 46 988 110 Fragments arranged by size: 1279 988 110 70 46 SphI G_CATG'C Cuts at: 0 2395 2493 Size: 2395 98 StyI C'CwwG_G Cuts at: 0 45 840 1296 2493 Size: 45 795 456 1197 Fragments arranged by size: 1197 795 456 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 708 1187 2493 Size: 708 479 1306 Fragments arranged by size: 1306 708 479 TatI w'GTAC_w Cuts at: 0 1514 2493 Size: 1514 979 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 238 270 530 562 2493 Size: 238 32 260 32 1931 Fragments arranged by size: 1931 260 238 32 32 Tth111I GACn'n_nGTC Cuts at: 0 274 2493 Size: 274 2219 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1497 2493 Size: 1497 996 XmnI GAAnn'nnTTC Cuts at: 0 1922 2493 Size: 1922 571 Enzymes that do cut: AccI Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI BanI BanII BbvCI BciVI BclI BfrBI BglI BglII BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI ClaI DraI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI NdeI NotI NsiI NspI PasI PflMI PfoI PpuMI PshAI PsrI PstI PvuII SacII SalI SanDI SfcI SphI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BcgI BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaBI BsiWI BspMI BssSI BstBI BstEII BstZ17I BtgZI BtsI CspCI DraIII DrdI EcoICRI FseI FspI FspAI HaeII KasI MfeI MluI NaeI NarI NgoMIV NheI NruI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI