(Linear) (Six-base) MAPSORT of: KIEE4491.seq Check: 8995 from: 1 to: 1536 KIEE4491.orf With 182 enzymes: SgfI * March 22, 2012 18:11 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 351 1536 Size: 130 221 1185 Fragments arranged by size: 1185 221 130 AhdI GACnn_n'nnGTC Cuts at: 0 94 1536 Size: 94 1442 AleI CACnn'nnGTG Cuts at: 0 244 1536 Size: 244 1292 AlwNI CAG_nnn'CTG Cuts at: 0 285 379 528 541 1536 Size: 285 94 149 13 995 Fragments arranged by size: 995 285 149 94 13 ApoI r'AATT_y Cuts at: 0 1435 1536 Size: 1435 101 BanI G'GyrC_C Cuts at: 0 404 1536 Size: 404 1132 BbeI G_GCGC'C Cuts at: 0 408 1536 Size: 408 1128 BbvCI CC'TCA_GC Cuts at: 0 500 1536 Size: 500 1036 BlpI GC'TnA_GC Cuts at: 0 256 643 1536 Size: 256 387 893 Fragments arranged by size: 893 387 256 Bme1580I G_kGCm'C Cuts at: 0 152 548 1536 Size: 152 396 988 Fragments arranged by size: 988 396 152 BmgBI CAC'GTC Cuts at: 0 216 1536 Size: 216 1320 BmrI ACTGGGnnnn_n' Cuts at: 0 1459 1536 Size: 1459 77 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1002 1138 1536 Size: 1002 136 398 Fragments arranged by size: 1002 398 136 Bpu10I CC'TnA_GC Cuts at: 0 500 1536 Size: 500 1036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 1536 Size: 69 1467 BsaI GGTCTCn'nnnn_ Cuts at: 0 1114 1372 1536 Size: 1114 258 164 Fragments arranged by size: 1114 258 164 BsaHI Gr'CG_yC Cuts at: 0 405 1536 Size: 405 1131 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 439 469 1536 Size: 439 30 1067 Fragments arranged by size: 1067 439 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 447 1048 1536 Size: 447 601 488 Fragments arranged by size: 601 488 447 BseYI C'CCAG_C Cuts at: 0 450 696 949 1391 1536 Size: 450 246 253 442 145 Fragments arranged by size: 450 442 253 246 145 BsiHKAI G_wGCw'C Cuts at: 0 806 863 1514 1536 Size: 806 57 651 22 Fragments arranged by size: 806 651 57 22 BsmI GAATG_Cn' Cuts at: 0 750 1536 Size: 750 786 Bsp1286I G_dGCh'C Cuts at: 0 152 548 806 863 1514 1536 Size: 152 396 258 57 651 22 Fragments arranged by size: 651 396 258 152 57 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1046 1536 Size: 1046 490 BsrBI CCG'CTC Cuts at: 0 260 1536 Size: 260 1276 BsrDI GCAATG_nn' Cuts at: 0 437 1382 1536 Size: 437 945 154 Fragments arranged by size: 945 437 154 BsrFI r'CCGG_y Cuts at: 0 314 401 611 1536 Size: 314 87 210 925 Fragments arranged by size: 925 314 210 87 BstXI CCAn_nnnn'nTGG Cuts at: 0 128 134 208 246 1536 Size: 128 6 74 38 1290 Fragments arranged by size: 1290 128 74 38 6 BstYI r'GATC_y Cuts at: 0 224 1025 1084 1536 Size: 224 801 59 452 Fragments arranged by size: 801 452 224 59 Bsu36I CC'TnA_GG Cuts at: 0 92 1536 Size: 92 1444 BtgI C'CryG_G Cuts at: 0 26 127 245 1536 Size: 26 101 118 1291 Fragments arranged by size: 1291 118 101 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 314 1536 Size: 314 1222 EaeI y'GGCC_r Cuts at: 0 23 609 1536 Size: 23 586 927 Fragments arranged by size: 927 586 23 EarI CTCTTCn'nnn_ Cuts at: 0 473 1140 1411 1536 Size: 473 667 271 125 Fragments arranged by size: 667 473 271 125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 351 1002 1138 1536 Size: 130 221 651 136 398 Fragments arranged by size: 651 398 221 136 130 EcoO109I rG'GnC_Cy Cuts at: 0 80 96 282 504 538 756 1536 Size: 80 16 186 222 34 218 780 Fragments arranged by size: 780 222 218 186 80 34 16 EcoRI G'AATT_C Cuts at: 0 1435 1536 Size: 1435 101 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 658 1536 Size: 658 878 FseI GG_CCGG'CC Cuts at: 0 318 1536 Size: 318 1218 HaeII r_GCGC'y Cuts at: 0 408 618 1536 Size: 408 210 918 Fragments arranged by size: 918 408 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 80 112 439 471 1536 Size: 80 32 327 32 1065 Fragments arranged by size: 1065 327 80 32 32 HincII GTy'rAC Cuts at: 0 654 1266 1536 Size: 654 612 270 Fragments arranged by size: 654 612 270 KasI G'GCGC_C Cuts at: 0 404 1536 Size: 404 1132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1152 1536 Size: 1152 384 MslI CAynn'nnrTG Cuts at: 0 244 1536 Size: 244 1292 MspA1I CmG'CkG Cuts at: 0 700 879 903 1391 1527 1536 Size: 700 179 24 488 136 9 Fragments arranged by size: 700 488 179 136 24 9 NaeI GCC'GGC Cuts at: 0 316 403 613 1536 Size: 316 87 210 923 Fragments arranged by size: 923 316 210 87 NarI GG'CG_CC Cuts at: 0 405 1536 Size: 405 1131 NcoI C'CATG_G Cuts at: 0 127 1536 Size: 127 1409 NgoMIV G'CCGG_C Cuts at: 0 314 401 611 1536 Size: 314 87 210 925 Fragments arranged by size: 925 314 210 87 NspI r_CATG'y Cuts at: 0 156 363 487 1501 1536 Size: 156 207 124 1014 35 Fragments arranged by size: 1014 207 156 124 35 PasI CC'CwG_GG Cuts at: 0 162 1010 1536 Size: 162 848 526 Fragments arranged by size: 848 526 162 PflMI CCAn_nnn'nTGG Cuts at: 0 379 1536 Size: 379 1157 PfoI T'CCnGG_A Cuts at: 0 833 979 1115 1536 Size: 833 146 136 421 Fragments arranged by size: 833 421 146 136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1280 1312 1536 Size: 1280 32 224 Fragments arranged by size: 1280 224 32 PpuMI rG'GwC_Cy Cuts at: 0 96 504 756 1536 Size: 96 408 252 780 Fragments arranged by size: 780 408 252 96 PshAI GACnn'nnGTC Cuts at: 0 586 1536 Size: 586 950 PstI C_TGCA'G Cuts at: 0 224 1536 Size: 224 1312 PvuII CAG'CTG Cuts at: 0 700 879 903 1527 1536 Size: 700 179 24 624 9 Fragments arranged by size: 700 624 179 24 9 SapI GCTCTTCn'nnn_ Cuts at: 0 473 1536 Size: 473 1063 SfcI C'TryA_G Cuts at: 0 220 558 774 1536 Size: 220 338 216 762 Fragments arranged by size: 762 338 220 216 SfoI GGC'GCC Cuts at: 0 406 1536 Size: 406 1130 SgrAI Cr'CCGG_yG Cuts at: 0 401 1536 Size: 401 1135 SmlI C'TyrA_G Cuts at: 0 84 1536 Size: 84 1452 SphI G_CATG'C Cuts at: 0 1501 1536 Size: 1501 35 StuI AGG'CCT Cuts at: 0 107 1108 1536 Size: 107 1001 428 Fragments arranged by size: 1001 428 107 StyI C'CwwG_G Cuts at: 0 127 1205 1536 Size: 127 1078 331 Fragments arranged by size: 1078 331 127 Tth111I GACn'n_nGTC Cuts at: 0 961 1536 Size: 961 575 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 125 246 1536 Size: 125 121 1290 Fragments arranged by size: 1290 125 121 XmnI GAAnn'nnTTC Cuts at: 0 430 748 1292 1536 Size: 430 318 544 244 Fragments arranged by size: 544 430 318 244 Enzymes that do cut: AcuI AhdI AleI AlwNI ApoI BanI BbeI BbvCI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI Eco57MI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PshAI PstI PvuII SapI SfcI SfoI SgrAI SmlI SphI StuI StyI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BmtI BplI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI XbaI XhoI XmaI ZraI