################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MluI MroI MslI MvnI NaeI NarI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 710 AAATTT [GA]^AATT[CT] Aor51HI 21 AGCGCT AGC^GCT ApoI 710 AAATTT [GA]^AATT[CT] Asp700I 663 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 5 CCCGGG C^[CT]CG[GA]G AvaII 328 GGACC G^G[AT]CC BalI 341 TGGCCA TGG^CCA BanI 1263 GGTGCC G^G[CT][GA]CC BciVI 1328 GTATCC GTATCC(6/5) BfaI 899 CTAG C^TAG BglII 504 AGATCT A^GATCT BlpI 17 GCTCAGC GC^T[ACGT]AGC BpmI 675 CTCCAG CTGGAG(16/14) Bpu1102I 17 GCTCAGC GC^T[ACGT]AGC BsaI 441 GAGACC GGTCTC(1/5) BsgI 289 CTGCAC GTGCAG(16/14) BsiEI 53 CGGCCG CG[GA][CT]^CG BsoBI 5 CCCGGG C^[CT]CG[GA]G BspHI 816 TCATGA T^CATGA BsrBI 1406 GAGCGG CCGCTC(-3/-3) BsrDI 756 CATTGC GCAATG(2/0) BstF5I 744 CATCC GGATG(2/0) CelII 17 GCTCAGC GC^T[ACGT]AGC EagI 53 CGGCCG C^GGCCG EarI 1424 CTCTTC CTCTTC(1/4) EclXI 53 CGGCCG C^GGCCG Eco47III 21 AGCGCT AGC^GCT Eco52I 53 CGGCCG C^GGCCG FauI 1412 CCCGC CCCGC(4/6) FokI 744 CATCC GGATG(9/13) HincII 413 GTCGAC GT[CT]^[GA]AC HindII 413 GTCGAC GT[CT]^[GA]AC Ksp632I 1424 CTCTTC CTCTTC(1/4) MaeI 899 CTAG C^TAG MluNI 341 TGGCCA TGG^CCA MscI 341 TGGCCA TGG^CCA MseI 1433 TTAA T^TAA MspA1I 50 CAGCGG C[AC]G^C[GT]G NdeI 919 CATATG CA^TATG NotI 52 GCGGCCGC GC^GGCCGC PflMI 319 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 816 TCATGA T^CATGA SacI 61 GAGCTC GAGCT^C SalI 413 GTCGAC G^TCGAC SapI 1423 GCTCTTC GCTCTTC(1/4) SfcI 426 CTACAG C^T[GA][CT]AG SmaI 5 CCCGGG CCC^GGG SmlI 212 CTCAAG C^T[CT][GA]AG SphI 403 GCATGC GCATG^C SspI 666 AATATT AAT^ATT Tru9I 1433 TTAA T^TAA Van91I 319 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1094 CCAAGAGGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 5 CCCGGG C^CCGGG XmaI 5 CCCGGG C^CCGGG XmnI 663 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 413 GTCGAC GT^[AC][GT]AC 545 GTCTAC AfaI 935 GTAC GT^AC 1239 GTAC AlwI 1273 GATCC GGATC(4/5) 532 GGATC AspHI 61 GAGCTC G[AT]GC[AT]^C 420 GTGCTC BanII 61 GAGCTC G[GA]GC[CT]^C 825 GGGCTC BclI 176 TGATCA T^GATCA 875 TGATCA BseRI 672 CTCCTC GAGGAG(10/8) 1234 GAGGAG BsiHKAI 61 GAGCTC G[AT]GC[AT]^C 420 GTGCTC BsmI 207 GAATGC GAATGC(1/-1) 253 GAATGC BsrI 1193 ACTGG ACTGG(1/-1) 1338 CCAGT BstXI 332 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1333 CCAAACCAGTGG Cfr13I 328 GGACC G^G[ACGT]CC 1410 GGCCC DsaI 332 CCATGG C^C[GA][CT]GG 863 CCATGG EaeI 53 CGGCCG [CT]^GGCC[GA] 341 TGGCCA FbaI 176 TGATCA T^GATCA 875 TGATCA HapII 6 CCGG C^CGG 1206 CCGG HpaII 6 CCGG C^CGG 1206 CCGG MspI 6 CCGG C^CGG 1206 CCGG NcoI 332 CCATGG C^CATGG 863 CCATGG NspI 133 GCATGT [GA]CATG^[CT] 403 GCATGC RsaI 935 GTAC GT^AC 1239 GTAC Sau96I 328 GGACC G^G[ACGT]CC 1410 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AluI BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstNI BstYI Cac8I CfoI DdeI DpnII EcoRII EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MfeI MflI MnlI MunI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII