##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        Tru9I       TseI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I         85  GGTACC               G^GTACC
AciI          306  CCGC                 CCGC(-3/-1)
AcsI          250  AAATTC               [GA]^AATT[CT]
ApoI          250  AAATTC               [GA]^AATT[CT]
Asp718I        85  GGTACC               G^GTACC
AvaII          98  GGACC                G^G[AT]CC
BanI           85  GGTACC               G^G[CT][GA]CC
BciVI          58  GGATAC               GTATCC(6/5)
BfaI          182  CTAG                 C^TAG
BpmI          239  CTCCAG               CTGGAG(16/14)
BsmI          244  GAATGC               GAATGC(1/-1)
Bsp1407I      434  TGTACA               T^GTACA
BsrGI         434  TGTACA               T^GTACA
BstF5I        165  CATCC                GGATG(2/0)
DpnII         285  GATC                 ^GATC
DsaI          143  CCGTGG               C^C[GA][CT]GG
EarI          127  CTCTTC               CTCTTC(1/4)
FokI          165  CATCC                GGATG(9/13)
HphI          205  GGTGA                GGTGA(8/7)
KpnI           85  GGTACC               GGTAC^C
Ksp632I       127  CTCTTC               CTCTTC(1/4)
MaeI          182  CTAG                 C^TAG
MaeIII         34  GTGAC                ^GT[ACGT]AC
MboI          285  GATC                 ^GATC
MslI           76  CACCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         285  GATC                 ^GATC
NheI          181  GCTAGC               G^CTAGC
PleI          341  GACTC                GAGTC(4/5)
PstI           67  CTGCAG               CTGCA^G
Sau3AI        285  GATC                 ^GATC
SfaNI          72  GATGC                GCATC(5/9)
SspBI         434  TGTACA               T^GTACA
Tsp45I         34  GTGAC                ^GT[GC]AC
TspRI         299  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI           20  AGCT                 AG^CT
              327  AGCT                
BalI          161  TGGCCA               TGG^CCA
              194  TGGCCA              
BsiYI          89  CCATTGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              186  CCTCTATGTGG         
BslI           89  CCATTGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              186  CCTCTATGTGG         
BsmFI          97  GGGAC                GGGAC(10/14)
              339  GGGAC               
Cfr13I         98  GGACC                G^G[ACGT]CC
              147  GGGCC               
EaeI          161  TGGCCA               [CT]^GGCC[GA]
              194  TGGCCA              
EcoT14I       101  CCAAGG               C^C[AT][AT]GG
              168  CCAAGG              
MluNI         161  TGGCCA               TGG^CCA
              194  TGGCCA              
MscI          161  TGGCCA               TGG^CCA
              194  TGGCCA              
NlaIV          85  GGTACC               GG[ACGT]^[ACGT]CC
               97  GGGACC              
Sau96I         98  GGACC                G^G[ACGT]CC
              147  GGGCC               
SfcI           67  CTGCAG               C^T[GA][CT]AG
              219  CTACAG              
StyI          101  CCAAGG               C^C[AT][AT]GG
              168  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AfaI        BsaJI       Cac8I       HaeIII      HinfI       
MboII       MnlI        NlaIII      RsaI        TfiI        
Tsp509I