################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BcgI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrI BssHII Bst1107I BstBI BstF5I BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluNI MroI MscI MseI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfaNI SfuI SgrAI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 977 ACGCGT A^C[GA][CT]GT AlwNI 415 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 395 AGCGCT AGC^GCT BanI 197 GGCGCC G^G[CT][GA]CC BbeI 197 GGCGCC GGCGC^C BbvCI 786 CCTCAGC CCTCAGC(-5/-2) BciVI 1188 GGATAC GTATCC(6/5) BfaI 403 CTAG C^TAG BglI 21 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 781 GGTCTC GGTCTC(1/5) BsmBI 541 GAGACG CGTCTC(1/5) BsmFI 1175 GTCCC GGGAC(10/14) Bsp1407I 995 TGTACA T^GTACA BspMI 695 ACCTGC ACCTGC(4/8) BsrBI 191 GAGCGG CCGCTC(-3/-3) BsrDI 146 GCAATG GCAATG(2/0) BsrFI 261 GCCGGT [GA]^CCGG[CT] BsrGI 995 TGTACA T^GTACA Cfr10I 261 GCCGGT [GA]^CCGG[CT] EaeI 509 TGGCCG [CT]^GGCC[GA] Eco47III 395 AGCGCT AGC^GCT KasI 197 GGCGCC G^GCGCC MaeI 403 CTAG C^TAG MluI 977 ACGCGT A^CGCGT NarI 197 GGCGCC GG^CGCC NcoI 506 CCATGG C^CATGG NruI 571 TCGCGA TCG^CGA PaeR7I 538 CTCGAG C^TCGAG PflMI 693 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1052 CAGCTG CAG^CTG SacI 268 GAGCTC GAGCT^C SfcI 273 CTACAG C^T[GA][CT]AG SfiI 20 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 197 GGCGCC GGC^GCC SmlI 538 CTCGAG C^T[CT][GA]AG SnaBI 964 TACGTA TAC^GTA SspBI 995 TGTACA T^GTACA StuI 381 AGGCCT AGG^CCT TaqI 539 TCGA T^CGA Tsp509I 1000 AATT ^AATT TthHB8I 539 TCGA T^CGA Van91I 693 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 538 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AvaII 452 GGACC G^G[AT]CC 1226 GGACC BamHI 863 GGATCC G^GATCC 1209 GGATCC BanII 268 GAGCTC G[GA]GC[CT]^C 562 GAGCCC BbrPI 163 CACGTG CAC^GTG 211 CACGTG BpmI 874 CTCCAG CTGGAG(16/14) 760 CTGGAG BssSI 343 CACGAG CACGAG(-5/-1) 474 CTCGTG BstAPI 162 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 658 GCACTAACTGC BstEII 1023 GGTGACC G^GT[ACGT]ACC 1154 GGTTACC BstPI 1023 GGTGACC G^GT[ACGT]ACC 1154 GGTTACC BstYI 863 GGATCC [GA]^GATC[CT] 1209 GGATCC DpnII 864 GATC ^GATC 1210 GATC DraIII 163 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 208 CACCACGTG EarI 1064 CTCTTC CTCTTC(1/4) 1102 CTCTTC EcoO65I 1023 GGTGACC G^GT[ACGT]ACC 1154 GGTTACC Ksp632I 1064 CTCTTC CTCTTC(1/4) 1102 CTCTTC MboI 864 GATC ^GATC 1210 GATC MflI 863 GGATCC [GA]^GATC[CT] 1209 GGATCC MslI 501 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1179 CACCCTAATG NdeII 864 GATC ^GATC 1210 GATC PmaCI 163 CACGTG CAC^GTG 211 CACGTG PmlI 163 CACGTG CAC^GTG 211 CACGTG Sau3AI 864 GATC ^GATC 1210 GATC SmaI 42 CCCGGG CCC^GGG 735 CCCGGG TspRI 686 CAGTG CA[GC]TG[ACGT][ACGT]^ 992 CACTG XhoII 863 GGATCC [GA]^GATC[CT] 1209 GGATCC XmaCI 42 CCCGGG C^CCGGG 735 CCCGGG XmaI 42 CCCGGG C^CCGGG 735 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI ApaLI AspHI AvaI BbsI BbvI BcnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DsaI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI RsaI SacII Sau96I ScrFI StyI TseI Tsp45I