Restriction map for
pFN21AE9533
(Circular) (Six-base) MAPSORT of: pFN21AE9533.seq Check: 4193 from: 1 to: 5935
pFN21AE9533 5935 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 5935
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3082 3186 3998 5382 5650 1632
Size: 566 884 104 812 1384 268 1917
Fragments arranged by size:
1917 1384 884 812 566 268 104
AfeI AGC'GCT
Cuts at: 2402 2402
Size: 5935
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 2982 4834 5811 1203
Size: 1779 1852 977 1327
Fragments arranged by size:
1852 1779 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2425 3678 5250 2425
Size: 1253 1572 3110
Fragments arranged by size:
3110 1572 1253
ApaLI G'TGCA_C
Cuts at: 2165 2345 2584 3992 5148 2165
Size: 180 239 1408 1156 2952
Fragments arranged by size:
2952 1408 1156 239 180
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2543 2740 3260 1870
Size: 73 104 496 197 520 4545
Fragments arranged by size:
4545 520 496 197 104 73
BamHI G'GATC_C
Cuts at: 2868 3214 3265 3632 2868
Size: 346 51 367 5171
Fragments arranged by size:
5171 367 346 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 3256 4711 618
Size: 325 217 627 415 1054 1455 1842
Fragments arranged by size:
1842 1455 1054 627 415 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2571 3254 1391
Size: 886 294 683 4072
Fragments arranged by size:
4072 886 683 294
BbeI G_GCGC'C
Cuts at: 2206 2206
Size: 5935
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 2819 3109 928
Size: 971 403 517 290 3754
Fragments arranged by size:
3754 971 517 403 290
BbvCI CC'TCA_GC
Cuts at: 2792 2792
Size: 5935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3187 3827 5037 3187
Size: 640 1210 4085
Fragments arranged by size:
4085 1210 640
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 4546 136
Size: 107 122 71 631 605 360 2514 1525
Fragments arranged by size:
2514 1525 631 605 360 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2054 2222 2375 3055 3352 2054
Size: 168 153 680 297 4637
Fragments arranged by size:
4637 680 297 168 153
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2349 2434 2588 3996 5152 1790
Size: 379 180 85 154 1408 1156 2573
Fragments arranged by size:
2573 1408 1156 379 180 154 85
BmgBI CAC'GTC
Cuts at: 1318 2860 1318
Size: 1542 4393
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2786 2864 4595 1239
Size: 1547 78 1731 2579
Fragments arranged by size:
2579 1731 1547 78
Bpu10I CC'TnA_GC
Cuts at: 2390 2792 2390
Size: 402 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4060 4925 5223 4060
Size: 865 298 4772
Fragments arranged by size:
4772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 4598 882
Size: 617 1293 1806 2219
Fragments arranged by size:
2219 1806 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 2170 2218 2971 3726 493
Size: 711 966 48 753 755 2702
Fragments arranged by size:
2702 966 755 753 711 48
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2203 2447 3742
Size: 53 83 186 936 531 139 244 1295
Cuts at: 3742 4124 4759 275
Size: 382 635 1451
Fragments arranged by size:
1451 1295 936 635 531 382 244 186
139 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2820 2850 1178
Size: 30 165 30 1417 30 4263
Fragments arranged by size:
4263 1417 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2795 3057 3082 1186
Size: 1594 15 262 25 4039
Fragments arranged by size:
4039 1594 262 25 15
BseYI C'CCAG_C
Cuts at: 2036 2071 2137 2756 5138 5634 2036
Size: 35 66 619 2382 496 2337
Fragments arranged by size:
2382 2337 619 496 66 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2223 2277 2349 2588 2655 3144 3996 4081
Size: 54 54 72 239 67 489 852 85
Cuts at: 4081 5152 2169
Size: 1071 2952
Fragments arranged by size:
2952 1071 852 489 239 85 72 67
54 54
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2540
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2223 2277 2349 2434 2571 2588
Size: 399 379 54 54 72 85 137 17
Cuts at: 2588 2655 3144 3254 3996 4081 5152 1391
Size: 67 489 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 379 137 110
85 85 72 67 54 54 17
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 3290 844
Size: 1865 581 3489
Fragments arranged by size:
3489 1865 581
BsrBI CCG'CTC
Cuts at: 1394 1823 2198 3822 1394
Size: 429 375 1624 3507
Fragments arranged by size:
3507 1624 429 375
BsrDI GCAATG_nn'
Cuts at: 65 1248 2158 4430 4604 65
Size: 1183 910 2272 174 1396
Fragments arranged by size:
2272 1396 1183 910 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2266 4579 4766 1700
Size: 110 456 2313 187 2869
Fragments arranged by size:
2869 2313 456 187 110
BsrGI T'GTAC_A
Cuts at: 96 3000 96
Size: 2904 3031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 2348 2479 3995 5007 2348
Size: 131 1516 1012 3276
Fragments arranged by size:
3276 1516 1012 131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 2669 794
Size: 1379 496 4060
Fragments arranged by size:
4060 1379 496
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 3028 3159 1184
Size: 1844 131 3960
Fragments arranged by size:
3960 1844 131
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 2764 3190 2512
Size: 252 426 5257
Fragments arranged by size:
5257 426 252
BstYI r'GATC_y
Cuts at: 1972 2868 3214 3265 3632 4017 4034 5486 5572
Size: 896 346 51 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 896 385 367 346 346 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 2030 2329 2367 2511 513
Size: 552 939 17 9 299 38 144 3937
Fragments arranged by size:
3937 939 552 299 144 38 17 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DraIII CAC_nnn'GTG
Cuts at: 2173 2218 2173
Size: 45 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2514 3382 4271
Size: 54 1425 50 275 18 684 868 889
Cuts at: 4271 5900 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 868 684 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3075 3113 3865 1353
Size: 1722 38 752 3423
Fragments arranged by size:
3423 1722 752 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2567 4522 4896 5042 387
Size: 1064 138 978 1955 374 146 1280
Fragments arranged by size:
1955 1280 1064 978 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2786 2864 3082 3186 3998 4595
Size: 393 566 588 78 218 104 812 597
Cuts at: 4595 5382 5650 1239
Size: 787 268 1524
Fragments arranged by size:
1524 812 787 597 588 566 393 268
218 104 78
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 5935
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 2138 2206 2234 2397 2404 2477 5082 1678
Size: 460 68 28 163 7 73 2605 2531
Fragments arranged by size:
2605 2531 460 163 73 68 28 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2820 2852 4576 4608 4650
Size: 32 135 32 1248 32 1724 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 1724 1248 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KasI G'GCGC_C
Cuts at: 2202 2202
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 2982 5811 2982
Size: 2829 3106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 5049 5233 1305
Size: 762 2982 184 2007
Fragments arranged by size:
2982 2007 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 3188 3893 4252 4411 518
Size: 648 1344 678 705 359 159 2042
Fragments arranged by size:
2042 1344 705 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2032 2331 3059 3351 4028 5176 5421
Size: 417 9 299 728 292 677 1148 245
Cuts at: 5421 1606
Size: 2120
Fragments arranged by size:
2120 1148 728 677 417 299 292 245
9
NarI GG'CG_CC
Cuts at: 2203 2203
Size: 5935
NcoI C'CATG_G
Cuts at: 513 1065 2004 2511 513
Size: 552 939 507 3937
Fragments arranged by size:
3937 939 552 507
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NruI TCG'CGA
Cuts at: 2578 2578
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2304 1348
Size: 956 4979
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4339
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 5935
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PmlI CAC'GTG
Cuts at: 2170 2218 2170
Size: 48 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3287 830
Size: 2457 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 3059 3059
Size: 5935
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5935
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 5935
SacII CC_GC'GG
Cuts at: 2024 2033 2332 2024
Size: 9 299 5627
Fragments arranged by size:
5627 299 9
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2278 3283 4418 5099 5290 826
Size: 219 1233 1005 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 1005 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2032 2032
Size: 5935
SfoI GGC'GCC
Cuts at: 2204 2204
Size: 5935
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2049 2742 3262 1872
Size: 177 693 520 4545
Fragments arranged by size:
4545 693 520 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 4039 4940 5202 820
Size: 197 926 600 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 600 262 197
SnaBI TAC'GTA
Cuts at: 493 2971 3726 493
Size: 2478 755 2702
Fragments arranged by size:
2702 2478 755
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 2388 1517
Size: 871 5064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2511 2658 3374 513
Size: 552 939 93 414 147 716 3074
Fragments arranged by size:
3074 939 716 552 414 147 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2961 3000 4181
Size: 274 80 33 51 1443 984 39 1181
Cuts at: 4181 5893 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1181 984 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 2543 1943
Size: 600 5335
XmaI C'CCGG_G
Cuts at: 1870 2047 2740 3260 1870
Size: 177 693 520 4545
Fragments arranged by size:
4545 693 520 177
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NsiI PacI PpuMI PspOMI PspXI PsrI SanDI SapI
SexAI SrfI SwaI XcmI