################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlpI Bpu1102I BpuAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII Hin1I HindIII HpaI HpaII KasI KspI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwNI 1584 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 898 ATTAAT AT^TAAT AsnI 898 ATTAAT AT^TAAT AspHI 1645 GTGCTC G[AT]GC[AT]^C AvrII 940 CCTAGG C^CTAGG BbrPI 1239 CACGTG CAC^GTG BglI 242 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 940 CCTAGG C^CTAGG BsaBI 1489 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 1645 GTGCTC G[AT]GC[AT]^C BspHI 1197 TCATGA T^CATGA BspMI 238 ACCTGC ACCTGC(4/8) BstXI 1227 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 259 AGATCC [GA]^GATC[CT] CfoI 1760 GCGC GCG^C DraIII 503 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1299 CCATGG C^C[GA][CT]GG EcoNI 62 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 409 GAATTC G^AATTC FauI 614 CCCGC CCCGC(4/6) HgaI 276 GACGC GACGC(5/10) HhaI 1760 GCGC GCG^C HinP1I 1760 GCGC G^CGC HincII 195 GTTGAC GT[CT]^[GA]AC HindII 195 GTTGAC GT[CT]^[GA]AC MamI 1489 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 144 CAATTG C^AATTG MflI 259 AGATCC [GA]^GATC[CT] MunI 144 CAATTG C^AATTG MwoI 242 GCCTCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 1299 CCATGG C^CATGG NspI 918 ACATGC [GA]CATG^[CT] PmaCI 1239 CACGTG CAC^GTG PmlI 1239 CACGTG CAC^GTG PshBI 898 ATTAAT AT^TAAT PstI 1401 CTGCAG CTGCA^G RcaI 1197 TCATGA T^CATGA SapI 28 GCTCTTC GCTCTTC(1/4) SexAI 1588 ACCTGGT A^CC[AT]GGT SnaBI 356 TACGTA TAC^GTA SpeI 451 ACTAGT A^CTAGT XhoII 259 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Acc65I 986 GGTACC G^GTACC 1592 GGTACC AfaI 987 GTAC GT^AC 1593 GTAC Asp718I 986 GGTACC G^GTACC 1592 GGTACC BanII 26 GGGCTC G[GA]GC[CT]^C 883 GAGCCC BsaAI 356 TACGTA [CT]AC^GT[GA] 1239 CACGTG Cac8I 129 GCCTGC GC[ACGT]^[ACGT]GC 719 GCAAGC KpnI 986 GGTACC GGTAC^C 1592 GGTACC PflMI 68 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1258 CCATCATGTGG PvuII 414 CAGCTG CAG^CTG 603 CAGCTG RsaI 987 GTAC GT^AC 1593 GTAC SfaNI 335 GATGC GCATC(5/9) 844 GATGC TfiI 861 GAATC G^A[AT]TC 1534 GATTC Van91I 68 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1258 CCATCATGTGG XcmI 916 CCACATGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1527 CCAATGTGATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BanI BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI