(Circular) (Six-base) MAPSORT of: pFN21AE4379.seq Check: 8104 from: 1 to: 5902 pFN21AE4379 5902 bp With 182 enzymes: SgfI * March 28, 2012 14:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2172 3141 3965 5349 5617 1632 Size: 540 969 824 1384 268 1917 Fragments arranged by size: 1917 1384 969 824 540 268 AflII C'TTAA_G Cuts at: 820 1017 2354 820 Size: 197 1337 4368 Fragments arranged by size: 4368 1337 197 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3020 3030 3645 5217 3020 Size: 10 615 1572 3705 Fragments arranged by size: 3705 1572 615 10 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2585 2852 3211 3437 3589 5698 1493 Size: 1092 267 359 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 359 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2880 3227 1870 Size: 73 937 347 4545 Fragments arranged by size: 4545 937 347 73 BamHI G'GATC_C Cuts at: 2805 3232 3599 2805 Size: 427 367 5108 Fragments arranged by size: 5108 427 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 2676 3221 1391 Size: 1285 545 4072 Fragments arranged by size: 4072 1285 545 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2439 2496 2969 928 Size: 971 279 261 57 473 3861 Fragments arranged by size: 3861 971 473 279 261 57 BbvCI CC'TCA_GC Cuts at: 3069 3069 Size: 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 2432 2965 Size: 416 53 83 399 24 913 102 533 Cuts at: 2965 3633 4591 442 Size: 668 958 1753 Fragments arranged by size: 1753 958 913 668 533 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2879 3098 4562 1239 Size: 1640 219 1464 2579 Fragments arranged by size: 2579 1640 1464 219 Bpu10I CC'TnA_GC Cuts at: 3069 3069 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 4027 4892 5190 2255 Size: 1772 865 298 2967 Fragments arranged by size: 2967 1772 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 3054 5896 3054 Size: 2842 3060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2723 3086 1186 Size: 1537 363 4002 Fragments arranged by size: 4002 1537 363 BseYI C'CCAG_C Cuts at: 5105 5601 5105 Size: 496 5406 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 3221 3963 4048 5119 1391 Size: 399 886 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 545 399 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2125 3257 844 Size: 1281 1132 3489 Fragments arranged by size: 3489 1281 1132 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 4397 4571 65 Size: 1183 1428 1721 174 1396 Fragments arranged by size: 1721 1428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2179 4546 4733 1700 Size: 110 369 2367 187 2869 Fragments arranged by size: 2869 2367 369 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 2478 4706 2478 Size: 2228 3674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2320 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2422 2805 2862 3232 3599 3984 4001 5453 Size: 450 383 57 370 367 385 17 1452 Cuts at: 5453 5539 5551 5897 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 450 385 383 370 367 346 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5350 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 2142 3206 2142 Size: 1064 4838 DraIII CAC_nnn'GTG Cuts at: 2815 2815 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867 Size: 54 1425 50 275 18 1519 889 1629 Cuts at: 5867 8 Size: 43 Fragments arranged by size: 1629 1519 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2058 2700 2825 3159 3832 1353 Size: 650 55 642 125 334 673 3423 Fragments arranged by size: 3423 673 650 642 334 125 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2879 3098 3141 3965 4562 5349 Size: 393 540 707 219 43 824 597 787 Cuts at: 5349 5617 1239 Size: 268 1524 Fragments arranged by size: 1524 824 787 707 597 540 393 268 219 43 EcoNI CCTnn'n_nnAGG Cuts at: 2053 2870 2053 Size: 817 5085 EcoO109I rG'GnC_Cy Cuts at: 2251 2251 Size: 5902 EcoRI G'AATT_C Cuts at: 2852 3211 3589 2852 Size: 359 378 5165 Fragments arranged by size: 5165 378 359 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2087 2324 2087 Size: 237 5665 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 5049 1678 Size: 3371 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 4543 4575 4617 Size: 32 135 32 596 32 2343 32 42 Cuts at: 4617 4649 1373 Size: 32 2626 Fragments arranged by size: 2626 2343 596 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3246 3492 669 Size: 988 281 709 599 246 3079 Fragments arranged by size: 3079 988 709 599 281 246 HindIII A'AGCT_T Cuts at: 748 2306 748 Size: 1558 4344 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2239 3086 5016 5200 1305 Size: 934 847 1930 184 2007 Fragments arranged by size: 2007 1930 934 847 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2396 2561 3318 3995 5143 5388 1606 Size: 790 165 757 677 1148 245 2120 Fragments arranged by size: 2120 1148 790 757 677 245 165 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5350 NdeI CA'TA_TG Cuts at: 387 2086 387 Size: 1699 4203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2815 2996 1108 Size: 1707 181 4014 Fragments arranged by size: 4014 1707 181 PfoI T'CCnGG_A Cuts at: 2856 2856 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2251 2251 Size: 5902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2396 2561 2396 Size: 165 5737 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2268 3250 4385 5066 5257 826 Size: 219 1223 982 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 982 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 2882 3229 1872 Size: 1010 347 4545 Fragments arranged by size: 4545 1010 347 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 2354 4006 4907 5169 820 Size: 197 926 291 120 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 291 262 197 120 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 3341 513 Size: 552 2276 3074 Fragments arranged by size: 3074 2276 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 3929 3946 4099 4284 4729 325 Size: 1255 535 1814 17 153 185 445 1498 Fragments arranged by size: 1814 1498 1255 535 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 3026 1316 Size: 1710 4192 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 2880 3227 1870 Size: 1010 347 4545 Fragments arranged by size: 4545 1010 347 XmnI GAAnn'nnTTC Cuts at: 2126 2966 4031 2126 Size: 840 1065 3997 Fragments arranged by size: 3997 1065 840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI