Restriction map for
pFN21AE6707
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# NO CUT ENZYMES ( 73 enzymes) #
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Acc65I AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BclI
BfrI BlnI BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraI EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AatII 2830 GACGTC GACGT^C
AcsI 316 AAATTC [GA]^AATT[CT]
AhdI 1510 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2486 AGCGCT AGC^GCT
ApoI 316 AAATTC [GA]^AATT[CT]
AspEI 1510 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2897 GGATCC G^GATCC
BbvCI 1261 GCTGAGG CCTCAGC(-5/-2)
BcgI 3229 GCATGGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2171 AGATCT A^GATCT
BspHI 2870 TCATGA T^CATGA
BssHII 2963 GCGCGC G^CGCGC
Bst1107I 3388 GTATAC GTA^TAC
BstZ17I 3388 GTATAC GTA^TAC
DrdI 2059 GACTTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1510 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2486 AGCGCT AGC^GCT
FseI 3196 GGCCGGCC GGCCGG^CC
KspI 3048 CCGCGG CCGC^GG
MseI 2474 TTAA T^TAA
RcaI 2870 TCATGA T^CATGA
SacI 1368 GAGCTC GAGCT^C
SacII 3048 CCGCGG CCGC^GG
SbfI 243 CCTGCAGG CCTGCA^GG
SexAI 50 ACCAGGT A^CC[AT]GGT
Sse8387I 243 CCTGCAGG CCTGCA^GG
TfiI 259 GAATC G^A[AT]TC
Tru9I 2474 TTAA T^TAA
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccIII 1800 TCCGGA T^CCGGA
2735 TCCGGA
AspHI 822 GAGCAC G[AT]GC[AT]^C
1368 GAGCTC
AspI 2830 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3145 GACGTGGTC
BbeI 1588 GGCGCC GGCGC^C
3136 GGCGCC
BbrPI 190 CACGTG CAC^GTG
1420 CACGTG
BfaI 98 CTAG C^TAG
2390 CTAG
BglI 1375 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2137 GCCCTGGAGGC
BsaAI 190 CACGTG [CT]AC^GT[GA]
1420 CACGTG
BsaWI 1800 TCCGGA [AT]^CCGG[AT]
2735 TCCGGA
BseAI 1800 TCCGGA T^CCGGA
2735 TCCGGA
BsiEI 2192 CGGCCG CG[GA][CT]^CG
3199 CGGCCG
BsiHKAI 822 GAGCAC G[AT]GC[AT]^C
1368 GAGCTC
BspEI 1800 TCCGGA T^CCGGA
2735 TCCGGA
BsrBI 384 GAGCGG CCGCTC(-3/-3)
2641 GAGCGG
DraIII 178 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3217 CACCGCGTG
EagI 2192 CGGCCG C^GGCCG
3199 CGGCCG
EclXI 2192 CGGCCG C^GGCCG
3199 CGGCCG
Eco52I 2192 CGGCCG C^GGCCG
3199 CGGCCG
KasI 1588 GGCGCC G^GCGCC
3136 GGCGCC
MaeI 98 CTAG C^TAG
2390 CTAG
MroI 1800 TCCGGA T^CCGGA
2735 TCCGGA
NaeI 1657 GCCGGC GCC^GGC
3197 GCCGGC
NarI 1588 GGCGCC GG^CGCC
3136 GGCGCC
NgoMIV 1657 GCCGGC G^CCGGC
3197 GCCGGC
PaeR7I 2215 CTCGAG C^TCGAG
3624 CTCGAG
PflFI 2830 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3145 GACGTGGTC
PmaCI 190 CACGTG CAC^GTG
1420 CACGTG
PmlI 190 CACGTG CAC^GTG
1420 CACGTG
SfoI 1588 GGCGCC GGC^GCC
3136 GGCGCC
SmlI 2215 CTCGAG C^T[CT][GA]AG
3624 CTCGAG
Tsp509I 317 AATT ^AATT
330 AATT
Tth111I 2830 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3145 GACGTGGTC
XcmI 2336 CCAGCCTCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2459 CCACCAGTGAGGTGG
XhoI 2215 CTCGAG C^TCGAG
3624 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SphI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI