(Circular) (Six-base) MAPSORT of: pFN21AE6707.seq Check: 1402 from: 1 to: 8434 pFN21AE6707 8434 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2312 2312 Size: 8434 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4839 6244 278 Size: 53 83 186 936 3303 1405 2468 Fragments arranged by size: 3303 2468 1405 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4869 4986 5394 5777 1937 Size: 2932 117 408 383 4594 Fragments arranged by size: 4594 2932 408 383 117 Acc65I G'GTAC_C Cuts at: 5755 5755 Size: 8434 AclI AA'CG_TT Cuts at: 1563 6561 6934 1563 Size: 4998 373 3063 Fragments arranged by size: 4998 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5059 5735 6497 7881 8149 1632 Size: 2300 1127 676 762 1384 268 1917 Fragments arranged by size: 2300 1917 1384 1127 762 676 268 AfeI AGC'GCT Cuts at: 4493 4493 Size: 8434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8237 AflIII A'CryG_T Cuts at: 1203 7333 8310 1203 Size: 6130 977 1327 Fragments arranged by size: 6130 1327 977 AgeI A'CCGG_T Cuts at: 7265 7265 Size: 8434 AhdI GACnn_n'nnGTC Cuts at: 3520 7163 3520 Size: 3643 4791 AleI CACnn'nnGTG Cuts at: 1166 4778 1166 Size: 3612 4822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3033 5547 1184 Size: 1849 2514 4071 Fragments arranged by size: 4071 2514 1849 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4736 4768 4736 Size: 32 8402 AlwNI CAG_nnn'CTG Cuts at: 3179 3332 3370 4593 4888 5163 5332 5476 6177 Size: 153 38 1223 295 275 169 144 701 Cuts at: 6177 7749 3179 Size: 1572 3864 Fragments arranged by size: 3864 1572 1223 701 295 275 169 153 144 38 ApaI G_GGCC'C Cuts at: 2160 2559 2671 3217 4830 5639 5663 2160 Size: 399 112 546 1613 809 24 4931 Fragments arranged by size: 4931 1613 809 546 399 112 24 ApaLI G'TGCA_C Cuts at: 6491 7647 6491 Size: 1156 7278 ApoI r'AATT_y Cuts at: 1493 2321 5743 5969 6121 8230 1493 Size: 828 3422 226 152 2109 1697 Fragments arranged by size: 3422 2109 1697 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 6988 160 Size: 872 5956 1606 Fragments arranged by size: 5956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2816 3021 3926 4220 4535 4933 Size: 73 216 657 205 905 294 315 398 Cuts at: 4933 5416 5539 5548 5629 5662 5759 1870 Size: 483 123 9 81 33 97 4545 Fragments arranged by size: 4545 905 657 483 398 315 294 216 205 123 97 81 73 33 9 BamHI G'GATC_C Cuts at: 4902 5764 6131 4902 Size: 862 367 7205 Fragments arranged by size: 7205 862 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2861 3442 3593 3659 3749 Size: 325 217 627 1074 581 151 66 90 Cuts at: 3749 4030 4372 4400 4913 5141 5755 7210 618 Size: 281 342 28 513 228 614 1455 1842 Fragments arranged by size: 1842 1455 1074 627 614 581 513 342 325 281 228 217 151 90 66 28 BanII G_rGCy'C Cuts at: 1391 2160 2204 2559 2671 3217 3377 3497 3927 Size: 769 44 355 112 546 160 120 430 Cuts at: 3927 3935 3984 4227 4830 5522 5639 5663 5753 Size: 8 49 243 603 692 117 24 90 Cuts at: 5753 1391 Size: 4072 Fragments arranged by size: 4072 769 692 603 546 430 355 243 160 120 117 112 90 49 44 24 8 BbeI G_GCGC'C Cuts at: 3597 5145 3597 Size: 1548 6886 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 3085 5065 928 Size: 971 472 714 1980 4297 Fragments arranged by size: 4297 1980 971 714 472 BbvCI CC'TCA_GC Cuts at: 3267 3267 Size: 8434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5223 5257 6625 6659 1903 Size: 34 50 34 3202 34 1368 34 3678 Fragments arranged by size: 3678 3202 1368 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6326 7536 6326 Size: 1210 7224 BclI T'GATC_A Cuts at: 1548 1548 Size: 8434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3386 4148 7045 Size: 107 122 71 631 605 1714 762 2897 Cuts at: 7045 136 Size: 1525 Fragments arranged by size: 2897 1714 1525 762 631 605 122 107 71 BglII A'GATC_T Cuts at: 4176 8429 4176 Size: 4253 4181 BlpI GC'TnA_GC Cuts at: 2711 3371 4805 5851 2711 Size: 660 1434 1046 5294 Fragments arranged by size: 5294 1434 1046 660 Bme1580I G_kGCm'C Cuts at: 1790 2160 2230 2536 2559 2671 2734 3217 3447 Size: 370 70 306 23 112 63 483 230 Cuts at: 3447 3752 4272 4377 4830 5639 5663 6495 7651 Size: 305 520 105 453 809 24 832 1156 Cuts at: 7651 1790 Size: 2573 Fragments arranged by size: 2573 1156 832 809 520 483 453 370 306 305 230 112 105 70 63 24 23 BmgBI CAC'GTC Cuts at: 1318 4609 5152 1318 Size: 3291 543 4600 Fragments arranged by size: 4600 3291 543 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 2611 3146 Size: 416 53 83 399 24 1122 72 535 Cuts at: 3146 3812 4112 4603 4946 6165 7123 442 Size: 666 300 491 343 1219 958 1753 Fragments arranged by size: 1753 1219 1122 958 666 535 491 416 399 343 300 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 3055 3817 2341 Size: 714 762 6958 Fragments arranged by size: 6958 762 714 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3201 3354 4166 4324 4373 4382 4508 4872 Size: 1962 153 812 158 49 9 126 364 Cuts at: 4872 7094 1239 Size: 2222 2579 Fragments arranged by size: 2579 2222 1962 812 364 158 153 126 49 9 Bpu10I CC'TnA_GC Cuts at: 2387 3267 3491 3921 2387 Size: 880 224 430 6900 Fragments arranged by size: 6900 880 430 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6559 7424 7722 6559 Size: 865 298 7271 Fragments arranged by size: 7271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2333 4078 5379 7097 882 Size: 617 834 1745 1301 1718 2219 Fragments arranged by size: 2219 1745 1718 1301 834 617 BsaAI yAC'GTr Cuts at: 493 1204 2197 3427 6225 493 Size: 711 993 1230 2798 2702 Fragments arranged by size: 2798 2702 1230 993 711 BsaBI GATnn'nnATC Cuts at: 8428 8428 Size: 8434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3339 3594 4836 5142 Size: 53 83 186 936 1806 255 1242 306 Cuts at: 5142 5265 6241 6623 7258 275 Size: 123 976 382 635 1451 Fragments arranged by size: 1806 1451 1242 976 936 635 382 306 255 186 123 83 53 BsaWI w'CCGG_w Cuts at: 3805 4740 6867 7265 7539 7686 8097 3805 Size: 935 2127 398 274 147 411 4142 Fragments arranged by size: 4142 2127 935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 2961 2991 3329 Size: 30 165 30 783 30 745 30 338 Cuts at: 3329 3359 3684 3714 4481 4511 4845 4875 1178 Size: 30 325 30 767 30 334 30 4737 Fragments arranged by size: 4737 783 767 745 338 334 325 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2416 2756 3101 3961 4370 4685 5527 Size: 925 305 340 345 860 409 315 842 Cuts at: 5527 5744 1186 Size: 217 3876 Fragments arranged by size: 3876 925 860 842 409 345 340 315 305 217 BseYI C'CCAG_C Cuts at: 2064 2763 2808 3259 3891 3902 4043 4162 4443 Size: 699 45 451 632 11 141 119 281 Cuts at: 4443 4457 4972 5404 5639 7637 8133 2064 Size: 14 515 432 235 1998 496 2365 Fragments arranged by size: 2365 1998 699 632 515 496 451 432 281 235 141 119 45 14 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 3383 5357 1163 Size: 573 1551 96 1974 4240 Fragments arranged by size: 4240 1974 1551 573 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4200 5207 5884 6645 7673 1540 Size: 275 186 2199 1007 677 761 1028 2301 Fragments arranged by size: 2301 2199 1028 1007 761 677 275 186 BsiHKAI G_wGCw'C Cuts at: 2831 3377 6495 6580 7651 2831 Size: 546 3118 85 1071 3614 Fragments arranged by size: 3614 3118 1071 546 85 BsmI GAATG_Cn' Cuts at: 2278 4670 5036 5945 6038 2278 Size: 2392 366 909 93 4674 Fragments arranged by size: 4674 2392 909 366 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3330 5110 5275 3330 Size: 1780 165 6489 Fragments arranged by size: 6489 1780 165 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2204 2230 2536 2559 2671 2734 Size: 399 370 44 26 306 23 112 63 Cuts at: 2734 2831 3217 3377 3447 3497 3752 3927 3935 Size: 97 386 160 70 50 255 175 8 Cuts at: 3935 3984 4227 4272 4377 4830 5522 5639 5663 Size: 49 243 45 105 453 692 117 24 Cuts at: 5663 5753 6495 6580 7651 1391 Size: 90 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 453 399 386 370 306 255 243 175 160 117 112 105 97 90 85 70 63 50 49 45 44 26 24 23 8 BspEI T'CCGG_A Cuts at: 3805 4740 8097 3805 Size: 935 3357 4142 Fragments arranged by size: 4142 3357 935 BspHI T'CATG_A Cuts at: 4875 6323 8053 4875 Size: 1448 1730 5256 Fragments arranged by size: 5256 1730 1448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 2312 2358 3667 4720 5789 844 Size: 1398 70 46 1309 1053 1069 3489 Fragments arranged by size: 3489 1398 1309 1069 1053 70 46 BsrBI CCG'CTC Cuts at: 1394 1823 2391 4648 6321 1394 Size: 429 568 2257 1673 3507 Fragments arranged by size: 3507 2257 1673 568 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3015 3609 5322 6929 7103 65 Size: 1183 1767 594 1713 1607 174 1396 Fragments arranged by size: 1767 1713 1607 1396 1183 594 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 3662 5202 5363 7078 7265 1700 Size: 110 329 1523 1540 161 1715 187 2869 Fragments arranged by size: 2869 1715 1540 1523 329 187 161 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8434 BssHII G'CGCG_C Cuts at: 1925 4968 1925 Size: 3043 5391 BssSI C'ACGA_G Cuts at: 6494 7506 6494 Size: 1012 7422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8434 BstBI TT'CG_AA Cuts at: 7238 7238 Size: 8434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8434 BstXI CCAn_nnnn'nTGG Cuts at: 2044 3910 4474 5386 5683 2044 Size: 1866 564 912 297 4795 Fragments arranged by size: 4795 1866 912 564 297 BstYI r'GATC_y Cuts at: 1972 2127 4176 4482 4737 4902 5764 6131 6516 Size: 155 2049 306 255 165 862 367 385 Cuts at: 6516 6533 7985 8071 8083 8429 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2049 1977 1452 862 385 367 346 306 255 165 155 86 17 12 BstZ17I GTA'TAC Cuts at: 5395 5395 Size: 8434 Bsu36I CC'TnA_GG Cuts at: 2877 3411 3654 2877 Size: 534 243 7657 Fragments arranged by size: 7657 534 243 BtgI C'CryG_G Cuts at: 513 1065 3597 3775 5053 5145 5260 5592 513 Size: 552 2532 178 1278 92 115 332 3355 Fragments arranged by size: 3355 2532 1278 552 332 178 115 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4237 487 Size: 683 56 3011 4684 Fragments arranged by size: 4684 3011 683 56 BtsI GCAGTG_nn' Cuts at: 2228 2451 2797 2933 3560 4050 4423 5471 5950 Size: 223 346 136 627 490 373 1048 479 Cuts at: 5950 6744 6764 2228 Size: 794 20 3898 Fragments arranged by size: 3898 1048 794 627 490 479 373 346 223 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3592 3627 529 Size: 35 3028 35 5336 Fragments arranged by size: 5336 3028 35 35 DraI TTT'AAA Cuts at: 5738 5738 Size: 8434 DraIII CAC_nnn'GTG Cuts at: 2188 5227 2188 Size: 3039 5395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4070 7441 809 Size: 507 2754 3371 1802 Fragments arranged by size: 3371 2754 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2772 2847 Size: 54 1425 50 275 18 303 639 75 Cuts at: 2847 3567 3600 3912 4197 4317 4425 4649 5204 Size: 720 33 312 285 120 108 224 555 Cuts at: 5204 5361 5881 6770 8399 8 Size: 157 520 889 1629 43 Fragments arranged by size: 1629 1425 889 720 639 555 520 312 303 285 275 224 157 120 108 75 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4197 5204 5881 1537 Size: 275 2385 1007 677 4090 Fragments arranged by size: 4090 2385 1007 677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2466 2733 3042 3296 4094 4193 4583 Size: 900 213 267 309 254 798 99 390 Cuts at: 4583 6364 1353 Size: 1781 3423 Fragments arranged by size: 3423 1781 900 798 390 309 267 254 213 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3367 3371 4591 4676 5663 7021 Size: 1064 138 1778 4 1220 85 987 1358 Cuts at: 7021 7395 7541 387 Size: 374 146 1280 Fragments arranged by size: 1778 1358 1280 1220 1064 987 374 146 138 85 4 EcoICRI GAG'CTC Cuts at: 3375 3375 Size: 8434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3201 3354 3932 4166 4324 4373 4382 Size: 393 1569 153 578 234 158 49 9 Cuts at: 4382 4508 4872 5059 5735 6497 7094 7881 8149 Size: 126 364 187 676 762 597 787 268 Cuts at: 8149 1239 Size: 1524 Fragments arranged by size: 1569 1524 787 762 676 597 578 393 364 268 234 187 158 153 126 49 9 EcoNI CCTnn'n_nnAGG Cuts at: 4628 4699 5336 4628 Size: 71 637 7726 Fragments arranged by size: 7726 637 71 EcoO109I rG'GnC_Cy Cuts at: 2476 2555 2556 2881 3213 3238 3329 3826 4827 Size: 79 1 325 332 25 91 497 1001 Cuts at: 4827 5543 5635 5636 5659 2476 Size: 716 92 1 23 5251 Fragments arranged by size: 5251 1001 716 497 332 325 92 91 79 25 23 1 1 EcoRI G'AATT_C Cuts at: 5743 6121 5743 Size: 378 8056 FseI GG_CCGG'CC Cuts at: 5206 5206 Size: 8434 FspI TGC'GCA Cuts at: 3277 4124 4997 5606 6940 3277 Size: 847 873 609 1334 4771 Fragments arranged by size: 4771 1334 873 847 609 FspAI rTGC'GCAy Cuts at: 4997 4997 Size: 8434 HaeII r_GCGC'y Cuts at: 1678 3434 3597 4495 4544 4816 5145 5405 7581 Size: 1756 163 898 49 272 329 260 2176 Cuts at: 7581 1678 Size: 2531 Fragments arranged by size: 2531 2176 1756 898 329 272 260 163 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2825 2857 3329 Size: 32 135 32 825 32 396 32 472 Cuts at: 3329 3361 3506 3538 4289 4321 4661 4693 7075 Size: 32 145 32 751 32 340 32 2382 Cuts at: 7075 7107 7149 7181 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2382 825 751 472 396 340 145 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5778 6024 669 Size: 988 281 3840 246 3079 Fragments arranged by size: 3840 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8434 HpaI GTT'AAC Cuts at: 6024 6024 Size: 8434 KasI G'GCGC_C Cuts at: 3593 5141 3593 Size: 1548 6886 KpnI G_GTAC'C Cuts at: 5759 5759 Size: 8434 MfeI C'AATT_G Cuts at: 6033 6033 Size: 8434 MluI A'CGCG_T Cuts at: 8310 8310 Size: 8434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3510 4779 7548 7732 1305 Size: 2205 1269 2769 184 2007 Fragments arranged by size: 2769 2205 2007 1269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2774 3569 3602 3914 Size: 54 1425 343 303 639 795 33 312 Cuts at: 3914 4427 10 Size: 513 4017 Fragments arranged by size: 4017 1425 795 639 513 343 312 303 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 4778 5022 5529 6392 6751 6910 518 Size: 648 3612 244 507 863 359 159 2042 Fragments arranged by size: 3612 2042 863 648 507 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2118 2169 3309 4016 4657 4888 5055 5509 Size: 512 51 1140 707 641 231 167 454 Cuts at: 5509 5620 5850 6527 7675 7920 1606 Size: 111 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 1140 707 677 641 512 454 245 231 230 167 111 51 NaeI GCC'GGC Cuts at: 3664 5204 3664 Size: 1540 6894 NarI GG'CG_CC Cuts at: 3594 5142 3594 Size: 1548 6886 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7882 NdeI CA'TA_TG Cuts at: 387 387 Size: 8434 NgoMIV G'CCGG_C Cuts at: 3662 5202 3662 Size: 1540 6894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8434 NotI GC'GGCC_GC Cuts at: 5881 5881 Size: 8434 NspI r_CATG'y Cuts at: 1132 1229 2611 2758 3010 3276 5534 5792 7337 Size: 97 1382 147 252 266 2258 258 1545 Cuts at: 7337 1132 Size: 2229 Fragments arranged by size: 2258 2229 1545 1382 266 258 252 147 97 PasI CC'CwG_GG Cuts at: 1348 3225 4291 4701 1348 Size: 1877 1066 410 5081 Fragments arranged by size: 5081 1877 1066 410 PciI A'CATG_T Cuts at: 7333 7333 Size: 8434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2770 3179 4593 1108 Size: 1662 409 1414 4949 Fragments arranged by size: 4949 1662 1414 409 PfoI T'CCnGG_A Cuts at: 2267 2415 3318 4485 2267 Size: 148 903 1167 6216 Fragments arranged by size: 6216 1167 903 148 PmeI GTTT'AAAC Cuts at: 5738 5738 Size: 8434 PmlI CAC'GTG Cuts at: 2197 3427 2197 Size: 1230 7204 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3035 3067 6503 6535 8016 8048 3035 Size: 32 3436 32 1481 32 3421 Fragments arranged by size: 3436 3421 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 3826 5543 2476 Size: 1350 1717 5367 Fragments arranged by size: 5367 1717 1350 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8434 PsiI TTA'TAA Cuts at: 6004 6004 Size: 8434 PspOMI G'GGCC_C Cuts at: 2156 2555 2667 3213 4826 5635 5659 2156 Size: 399 112 546 1613 809 24 4931 Fragments arranged by size: 4931 1613 809 546 399 112 24 PspXI vC'TCGA_Gb Cuts at: 4220 5629 4220 Size: 1409 7025 PstI C_TGCA'G Cuts at: 830 2253 4332 5328 5786 830 Size: 1423 2079 996 458 3478 Fragments arranged by size: 3478 2079 1423 996 458 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8434 PvuII CAG'CTG Cuts at: 4657 5509 5620 4657 Size: 852 111 7471 Fragments arranged by size: 7471 852 111 SacI G_AGCT'C Cuts at: 3377 3377 Size: 8434 SacII CC_GC'GG Cuts at: 5056 5056 Size: 8434 SalI G'TCGA_C Cuts at: 1936 5776 1936 Size: 3840 4594 SanDI GG'GwC_CC Cuts at: 3826 5543 3826 Size: 1717 6717 SbfI CC_TGCA'GG Cuts at: 2253 5786 2253 Size: 3533 4901 ScaI AGT'ACT Cuts at: 6682 8394 6682 Size: 1712 6722 SexAI A'CCwGG_T Cuts at: 2055 2055 Size: 8434 SfcI C'TryA_G Cuts at: 826 1045 2249 4328 4684 5324 5456 5782 6917 Size: 219 1204 2079 356 640 132 326 1135 Cuts at: 6917 7598 7789 826 Size: 681 191 1471 Fragments arranged by size: 2079 1471 1204 1135 681 640 356 326 219 191 132 SfoI GGC'GCC Cuts at: 3595 5143 3595 Size: 1548 6886 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8434 SmaI CCC'GGG Cuts at: 1872 2161 2818 5664 5761 1872 Size: 289 657 2846 97 4545 Fragments arranged by size: 4545 2846 657 289 97 SmlI C'TyrA_G Cuts at: 820 1017 1943 4220 5629 6538 7439 7701 820 Size: 197 926 2277 1409 909 901 262 1553 Fragments arranged by size: 2277 1553 1409 926 909 901 262 197 SnaBI TAC'GTA Cuts at: 493 6225 493 Size: 5732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8434 SphI G_CATG'C Cuts at: 1132 2611 2758 3010 3276 5534 5792 1132 Size: 1479 147 252 266 2258 258 3774 Fragments arranged by size: 3774 2258 1479 266 258 252 147 SspI AAT'ATT Cuts at: 5 52 6358 5 Size: 47 6306 2081 Fragments arranged by size: 6306 2081 47 StuI AGG'CCT Cuts at: 1517 2681 3416 3629 3852 1517 Size: 1164 735 213 223 6099 Fragments arranged by size: 6099 1164 735 223 213 StyI C'CwwG_G Cuts at: 513 1065 2238 3099 3417 5127 5559 5873 513 Size: 552 1173 861 318 1710 432 314 3074 Fragments arranged by size: 3074 1710 1173 861 552 432 318 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2485 3238 3469 4399 5306 5689 6461 Size: 1255 905 753 231 930 907 383 772 Cuts at: 6461 6478 6631 6816 7261 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 930 907 905 772 753 445 383 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 4527 6680 Size: 274 80 33 51 1443 483 2067 2153 Cuts at: 6680 8392 96 Size: 1712 138 Fragments arranged by size: 2153 2067 1712 1443 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2186 2218 2961 2993 4402 4434 4481 4513 4537 Size: 32 743 32 1409 32 47 32 24 Cuts at: 4537 4569 4845 4877 8394 8426 2186 Size: 32 276 32 3517 32 2194 Fragments arranged by size: 3517 2194 1409 743 276 47 32 32 32 32 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 1316 4838 5153 1316 Size: 3522 315 4597 Fragments arranged by size: 4597 3522 315 XbaI T'CTAG_A Cuts at: 5770 5770 Size: 8434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4348 4471 4348 Size: 123 8311 XhoI C'TCGA_G Cuts at: 1943 4220 5629 1943 Size: 2277 1409 4748 Fragments arranged by size: 4748 2277 1409 XmaI C'CCGG_G Cuts at: 1870 2159 2816 5662 5759 1870 Size: 289 657 2846 97 4545 Fragments arranged by size: 4545 2846 657 289 97 XmnI GAAnn'nnTTC Cuts at: 6563 6563 Size: 8434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4837 6242 276 Size: 53 83 186 936 3303 1405 2468 Fragments arranged by size: 3303 2468 1405 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI EcoRV FalI NruI NsiI PacI PsrI RsrII SapI SfiI SrfI SwaI