(Circular) (Six-base) MAPSORT of: pFN21AE5718.seq Check: 4556 from: 1 to: 7618 pFN21AE5718 7618 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5428 278 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 4961 1937 Size: 2379 645 4594 Fragments arranged by size: 4594 2379 645 Acc65I G'GTAC_C Cuts at: 4939 4939 Size: 7618 AclI AA'CG_TT Cuts at: 1563 5745 6118 1563 Size: 4182 373 3063 Fragments arranged by size: 4182 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2360 2753 2908 3153 4751 5681 7065 Size: 464 264 393 155 245 1598 930 1384 Cuts at: 7065 7333 1632 Size: 268 1917 Fragments arranged by size: 1917 1598 1384 930 464 393 268 264 245 155 AflII C'TTAA_G Cuts at: 820 1017 3556 820 Size: 197 2539 4882 Fragments arranged by size: 4882 2539 197 AflIII A'CryG_T Cuts at: 1203 6517 7494 1203 Size: 5314 977 1327 Fragments arranged by size: 5314 1327 977 AgeI A'CCGG_T Cuts at: 6449 6449 Size: 7618 AhdI GACnn_n'nnGTC Cuts at: 2016 6347 2016 Size: 4331 3287 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7618 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3246 3278 4082 4114 3246 Size: 32 804 32 6750 Fragments arranged by size: 6750 804 32 32 AlwNI CAG_nnn'CTG Cuts at: 3778 3800 5361 6933 3778 Size: 22 1561 1572 4463 Fragments arranged by size: 4463 1572 1561 22 ApaLI G'TGCA_C Cuts at: 5675 6831 5675 Size: 1156 6462 ApoI r'AATT_y Cuts at: 1493 2114 2493 2874 2883 3298 3581 4927 5153 Size: 621 379 381 9 415 283 1346 226 Cuts at: 5153 5305 7414 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1346 621 415 381 379 283 226 152 9 AseI AT'TA_AT Cuts at: 160 1032 6172 160 Size: 872 5140 1606 Fragments arranged by size: 5140 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 AvaI C'yCGr_G Cuts at: 1870 1943 4943 1870 Size: 73 3000 4545 Fragments arranged by size: 4545 3000 73 BamHI G'GATC_C Cuts at: 4079 4526 4948 5315 4079 Size: 447 422 367 6382 Fragments arranged by size: 6382 447 422 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 3695 4939 6394 618 Size: 325 217 627 1505 403 1244 1455 1842 Fragments arranged by size: 1842 1505 1455 1244 627 403 325 217 BanII G_rGCy'C Cuts at: 1391 2521 2739 4326 4937 1391 Size: 1130 218 1587 611 4072 Fragments arranged by size: 4072 1587 1130 611 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5809 5843 1903 Size: 34 50 34 3788 34 3678 Fragments arranged by size: 3788 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2979 5510 6720 2979 Size: 2531 1210 3877 Fragments arranged by size: 3877 2531 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7618 BfrBI ATG'CAT Cuts at: 3807 3807 Size: 7618 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4046 6229 136 Size: 107 122 71 631 605 2374 2183 1525 Fragments arranged by size: 2374 2183 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3010 3840 4249 7613 3010 Size: 830 409 3364 3015 Fragments arranged by size: 3364 3015 830 409 BlpI GC'TnA_GC Cuts at: 4776 5035 4776 Size: 259 7359 Bme1580I G_kGCm'C Cuts at: 1790 3700 5679 6835 1790 Size: 1910 1979 1156 2573 Fragments arranged by size: 2573 1979 1910 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3835 5349 6307 Size: 416 53 83 399 24 2418 1514 958 Cuts at: 6307 442 Size: 1753 Fragments arranged by size: 2418 1753 1514 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7618 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3873 3873 Size: 7618 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2900 3641 6278 1239 Size: 1661 741 2637 2579 Fragments arranged by size: 2637 2579 1661 741 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3178 4274 5743 6608 6906 3178 Size: 1096 1469 865 298 3890 Fragments arranged by size: 3890 1469 1096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6281 882 Size: 617 4782 2219 Fragments arranged by size: 4782 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5409 493 Size: 711 4205 2702 Fragments arranged by size: 4205 2702 711 BsaBI GATnn'nnATC Cuts at: 7612 7612 Size: 7618 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5425 5807 6442 275 Size: 53 83 186 936 3892 382 635 1451 Fragments arranged by size: 3892 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4076 6051 6449 6723 6870 7281 4076 Size: 1975 398 274 147 411 4413 Fragments arranged by size: 4413 1975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2283 2313 2619 2649 3617 Size: 30 165 30 880 30 306 30 968 Cuts at: 3617 3647 4082 4112 1178 Size: 30 435 30 4684 Fragments arranged by size: 4684 968 880 435 306 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2163 3638 3876 4106 4109 4382 4883 1186 Size: 977 1475 238 230 3 273 501 3921 Fragments arranged by size: 3921 1475 977 501 273 238 230 3 BseYI C'CCAG_C Cuts at: 2462 6821 7317 2462 Size: 4359 496 2763 Fragments arranged by size: 4359 2763 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 3408 1163 Size: 573 312 1360 5373 Fragments arranged by size: 5373 1360 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5068 5829 6857 1540 Size: 275 186 3067 761 1028 2301 Fragments arranged by size: 3067 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4326 5679 5764 6835 4326 Size: 1353 85 1071 5109 Fragments arranged by size: 5109 1353 1071 85 BsmI GAATG_Cn' Cuts at: 2097 3809 4063 4300 5129 5222 2097 Size: 1712 254 237 829 93 4493 Fragments arranged by size: 4493 1712 829 254 237 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3678 3678 Size: 7618 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2521 2739 3700 4326 4937 5679 5764 Size: 399 731 218 961 626 611 742 85 Cuts at: 5764 6835 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 961 742 731 626 611 399 218 85 BspEI T'CCGG_A Cuts at: 7281 7281 Size: 7618 BspHI T'CATG_A Cuts at: 2089 5507 7237 2089 Size: 3418 1730 2470 Fragments arranged by size: 3418 2470 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 4973 844 Size: 1235 2894 3489 Fragments arranged by size: 3489 2894 1235 BsrBI CCG'CTC Cuts at: 1394 1823 2616 5505 1394 Size: 429 793 2889 3507 Fragments arranged by size: 3507 2889 793 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 6113 6287 65 Size: 1183 881 3984 174 1396 Fragments arranged by size: 3984 1396 1183 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6262 6449 1700 Size: 110 4452 187 2869 Fragments arranged by size: 4452 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7618 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7618 BssSI C'ACGA_G Cuts at: 2354 3788 5678 6690 2354 Size: 1434 1890 1012 3282 Fragments arranged by size: 3282 1890 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7618 BstBI TT'CG_AA Cuts at: 2887 6422 2887 Size: 3535 4083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7618 BstXI CCAn_nnnn'nTGG Cuts at: 3040 3040 Size: 7618 BstYI r'GATC_y Cuts at: 1972 3010 3840 4079 4249 4526 4948 5315 5700 Size: 1038 830 239 170 277 422 367 385 Cuts at: 5700 5717 7169 7255 7267 7613 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1038 830 422 385 367 346 277 239 170 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2138 2157 2416 2593 4035 513 Size: 552 1073 19 259 177 1442 4096 Fragments arranged by size: 4096 1442 1073 552 259 177 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 487 Size: 683 56 2027 4852 Fragments arranged by size: 4852 2027 683 56 BtsI GCAGTG_nn' Cuts at: 2212 5134 5928 5948 2212 Size: 2922 794 20 3882 Fragments arranged by size: 3882 2922 794 20 ClaI AT'CG_AT Cuts at: 1554 2943 1554 Size: 1389 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2342 2377 529 Size: 35 1778 35 5770 Fragments arranged by size: 5770 1778 35 35 DraI TTT'AAA Cuts at: 2343 4922 2343 Size: 2579 5039 DraIII CAC_nnn'GTG Cuts at: 3289 3289 Size: 7618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2803 6625 809 Size: 507 1487 3822 1802 Fragments arranged by size: 3822 1802 1487 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3816 4038 5065 Size: 54 1425 50 275 18 1986 222 1027 Cuts at: 5065 5954 7583 8 Size: 889 1629 43 Fragments arranged by size: 1986 1629 1425 1027 889 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5065 1537 Size: 275 3253 4090 Fragments arranged by size: 4090 3253 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2808 3129 3596 4331 4727 5548 1353 Size: 897 558 321 467 735 396 821 3423 Fragments arranged by size: 3423 897 821 735 558 467 396 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6205 6579 6725 387 Size: 1064 138 4616 374 146 1280 Fragments arranged by size: 4616 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4324 4324 Size: 7618 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2360 2753 2900 2908 3153 3641 Size: 393 464 264 393 147 8 245 488 Cuts at: 3641 4751 5681 6278 7065 7333 1239 Size: 1110 930 597 787 268 1524 Fragments arranged by size: 1524 1110 930 787 597 488 464 393 393 268 264 245 147 8 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 7618 EcoO109I rG'GnC_Cy Cuts at: 4879 4879 Size: 7618 EcoRI G'AATT_C Cuts at: 2114 2493 2874 3581 4927 5305 2114 Size: 379 381 707 1346 378 4427 Fragments arranged by size: 4427 1346 707 381 379 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4796 4796 Size: 7618 FspI TGC'GCA Cuts at: 2939 6124 2939 Size: 3185 4433 FspAI rTGC'GCAy Cuts at: 2939 2939 Size: 7618 HaeII r_GCGC'y Cuts at: 1678 6765 1678 Size: 5087 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2469 2501 2590 Size: 32 135 32 430 32 435 32 89 Cuts at: 2590 2622 2807 2839 2945 2977 3206 3238 3443 Size: 32 185 32 106 32 229 32 205 Cuts at: 3443 3475 3941 3973 4562 4594 6259 6291 6333 Size: 32 466 32 589 32 1665 32 42 Cuts at: 6333 6365 1373 Size: 32 2626 Fragments arranged by size: 2626 1665 589 466 435 430 229 205 185 135 106 89 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 2958 4962 5208 669 Size: 988 281 568 452 2004 246 3079 Fragments arranged by size: 3079 2004 988 568 452 281 246 HindIII A'AGCT_T Cuts at: 748 2529 748 Size: 1781 5837 HpaI GTT'AAC Cuts at: 5208 5208 Size: 7618 KpnI G_GTAC'C Cuts at: 4943 4943 Size: 7618 MfeI C'AATT_G Cuts at: 2232 3639 5217 2232 Size: 1407 1578 4633 Fragments arranged by size: 4633 1578 1407 MluI A'CGCG_T Cuts at: 7494 7494 Size: 7618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6732 6916 1305 Size: 5427 184 2007 Fragments arranged by size: 5427 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3818 4040 10 Size: 54 1425 343 1986 222 3588 Fragments arranged by size: 3588 1986 1425 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2628 3038 5576 5935 6094 518 Size: 648 1462 410 2538 359 159 2042 Fragments arranged by size: 2538 2042 1462 648 410 359 159 MspA1I CmG'CkG Cuts at: 1606 2195 5034 5711 6859 7104 1606 Size: 589 2839 677 1148 245 2120 Fragments arranged by size: 2839 2120 1148 677 589 245 NcoI C'CATG_G Cuts at: 513 1065 2157 2416 4035 513 Size: 552 1092 259 1619 4096 Fragments arranged by size: 4096 1619 1092 552 259 NdeI CA'TA_TG Cuts at: 387 387 Size: 7618 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7618 NotI GC'GGCC_GC Cuts at: 5065 5065 Size: 7618 NsiI A_TGCA'T Cuts at: 3809 3809 Size: 7618 NspI r_CATG'y Cuts at: 1132 1229 3370 4339 4976 6521 1132 Size: 97 2141 969 637 1545 2229 Fragments arranged by size: 2229 2141 1545 969 637 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7618 PciI A'CATG_T Cuts at: 6517 6517 Size: 7618 PflMI CCAn_nnn'nTGG Cuts at: 1108 3825 4439 1108 Size: 2717 614 4287 Fragments arranged by size: 4287 2717 614 PfoI T'CCnGG_A Cuts at: 2916 3915 4890 2916 Size: 999 975 5644 Fragments arranged by size: 5644 999 975 PmeI GTTT'AAAC Cuts at: 4922 4922 Size: 7618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5687 5719 7200 7232 4082 Size: 32 1573 32 1481 32 4468 Fragments arranged by size: 4468 1573 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4879 4879 Size: 7618 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7618 PsiI TTA'TAA Cuts at: 5188 5188 Size: 7618 PstI C_TGCA'G Cuts at: 830 4970 830 Size: 4140 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7618 PvuII CAG'CTG Cuts at: 2195 2195 Size: 7618 SacI G_AGCT'C Cuts at: 4326 4326 Size: 7618 SalI G'TCGA_C Cuts at: 1936 4960 1936 Size: 3024 4594 SapI GCTCTTCn'nnn_ Cuts at: 3596 4331 3596 Size: 735 6883 SbfI CC_TGCA'GG Cuts at: 4970 4970 Size: 7618 ScaI AGT'ACT Cuts at: 5866 7578 5866 Size: 1712 5906 SfcI C'TryA_G Cuts at: 826 1045 2745 4966 6101 6782 6973 826 Size: 219 1700 2221 1135 681 191 1471 Fragments arranged by size: 2221 1700 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4046 4046 Size: 7618 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7618 SmaI CCC'GGG Cuts at: 1872 4945 1872 Size: 3073 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3157 3556 4253 5722 6623 6885 Size: 197 926 1214 399 697 1469 901 262 Cuts at: 6885 820 Size: 1553 Fragments arranged by size: 1553 1469 1214 926 901 697 399 262 197 SnaBI TAC'GTA Cuts at: 493 5409 493 Size: 4916 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7618 SphI G_CATG'C Cuts at: 1132 4976 1132 Size: 3844 3774 SspI AAT'ATT Cuts at: 5 52 5542 5 Size: 47 5490 2081 Fragments arranged by size: 5490 2081 47 StuI AGG'CCT Cuts at: 1517 3866 4631 1517 Size: 2349 765 4504 Fragments arranged by size: 4504 2349 765 StyI C'CwwG_G Cuts at: 513 1065 2157 2416 2861 4035 4433 5057 513 Size: 552 1092 259 445 1174 398 624 3074 Fragments arranged by size: 3074 1174 1092 624 552 445 398 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 5645 5662 5815 6000 6445 325 Size: 1255 2088 1977 17 153 185 445 1498 Fragments arranged by size: 2088 1977 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4065 4174 4338 Size: 274 80 33 51 1443 2088 109 164 Cuts at: 4338 5864 7576 96 Size: 1526 1712 138 Fragments arranged by size: 2088 1712 1526 1443 274 164 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 2970 3002 3617 3649 4435 4467 7578 Size: 32 655 32 615 32 786 32 3111 Cuts at: 7578 7610 2283 Size: 32 2291 Fragments arranged by size: 3111 2291 786 655 615 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7618 XbaI T'CTAG_A Cuts at: 4864 4954 4864 Size: 90 7528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3114 3775 3967 3114 Size: 661 192 6765 Fragments arranged by size: 6765 661 192 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7618 XmaI C'CCGG_G Cuts at: 1870 4943 1870 Size: 3073 4545 XmnI GAAnn'nnTTC Cuts at: 2439 4159 4707 5747 2439 Size: 1720 548 1040 4310 Fragments arranged by size: 4310 1720 1040 548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5426 276 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI