(Linear) (Six-base) MAPSORT of: KIEE5718.seq Check: 9153 from: 1 to: 2913 KIEE5718.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 2311 2913 Size: 2311 602 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 355 748 903 1148 2746 2913 Size: 91 264 393 155 245 1598 167 Fragments arranged by size: 1598 393 264 245 167 155 91 AflII C'TTAA_G Cuts at: 0 1551 2913 Size: 1551 1362 AhdI GACnn_n'nnGTC Cuts at: 0 11 2913 Size: 11 2902 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1241 1273 2077 2109 2913 Size: 1241 32 804 32 804 Fragments arranged by size: 1241 804 804 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1773 1795 2913 Size: 1773 22 1118 Fragments arranged by size: 1773 1118 22 ApoI r'AATT_y Cuts at: 0 109 488 869 878 1293 1576 2913 Size: 109 379 381 9 415 283 1337 Fragments arranged by size: 1337 415 381 379 283 109 9 BamHI G'GATC_C Cuts at: 0 2074 2521 2913 Size: 2074 447 392 Fragments arranged by size: 2074 447 392 BanI G'GyrC_C Cuts at: 0 1287 1690 2913 Size: 1287 403 1223 Fragments arranged by size: 1287 1223 403 BanII G_rGCy'C Cuts at: 0 516 734 2321 2913 Size: 516 218 1587 592 Fragments arranged by size: 1587 592 516 218 BciVI GTATCCnnnnn_n' Cuts at: 0 974 2913 Size: 974 1939 BfrBI ATG'CAT Cuts at: 0 1802 2913 Size: 1802 1111 BglI GCCn_nnn'nGGC Cuts at: 0 2041 2913 Size: 2041 872 BglII A'GATC_T Cuts at: 0 1005 1835 2244 2913 Size: 1005 830 409 669 Fragments arranged by size: 1005 830 669 409 BlpI GC'TnA_GC Cuts at: 0 2771 2913 Size: 2771 142 Bme1580I G_kGCm'C Cuts at: 0 1695 2913 Size: 1695 1218 BmrI ACTGGGnnnn_n' Cuts at: 0 1830 2913 Size: 1830 1083 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1868 2913 Size: 1868 1045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 1636 2913 Size: 895 741 1277 Fragments arranged by size: 1277 895 741 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1173 2269 2913 Size: 1173 1096 644 Fragments arranged by size: 1173 1096 644 BsaWI w'CCGG_w Cuts at: 0 2071 2913 Size: 2071 842 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 278 308 614 644 1612 1642 2077 2107 Size: 278 30 306 30 968 30 435 30 Cuts at: 2107 2913 Size: 806 Fragments arranged by size: 968 806 435 306 278 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 158 1633 1871 2101 2104 2377 2878 2913 Size: 158 1475 238 230 3 273 501 35 Fragments arranged by size: 1475 501 273 238 230 158 35 3 BseYI C'CCAG_C Cuts at: 0 457 2913 Size: 457 2456 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 1403 2913 Size: 43 1360 1510 Fragments arranged by size: 1510 1360 43 BsiHKAI G_wGCw'C Cuts at: 0 2321 2913 Size: 2321 592 BsmI GAATG_Cn' Cuts at: 0 92 1804 2058 2295 2913 Size: 92 1712 254 237 618 Fragments arranged by size: 1712 618 254 237 92 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1673 2913 Size: 1673 1240 Bsp1286I G_dGCh'C Cuts at: 0 516 734 1695 2321 2913 Size: 516 218 961 626 592 Fragments arranged by size: 961 626 592 516 218 BspHI T'CATG_A Cuts at: 0 84 2913 Size: 84 2829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 2913 Size: 74 2839 BsrBI CCG'CTC Cuts at: 0 611 2913 Size: 611 2302 BsrDI GCAATG_nn' Cuts at: 0 124 2913 Size: 124 2789 BssSI C'ACGA_G Cuts at: 0 349 1783 2913 Size: 349 1434 1130 Fragments arranged by size: 1434 1130 349 BstBI TT'CG_AA Cuts at: 0 882 2913 Size: 882 2031 BstXI CCAn_nnnn'nTGG Cuts at: 0 1035 2913 Size: 1035 1878 BstYI r'GATC_y Cuts at: 0 1005 1835 2074 2244 2521 2913 Size: 1005 830 239 170 277 392 Fragments arranged by size: 1005 830 392 277 239 170 BtgI C'CryG_G Cuts at: 0 133 152 411 588 2030 2913 Size: 133 19 259 177 1442 883 Fragments arranged by size: 1442 883 259 177 133 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1248 2913 Size: 1248 1665 BtsI GCAGTG_nn' Cuts at: 0 207 2913 Size: 207 2706 ClaI AT'CG_AT Cuts at: 0 938 2913 Size: 938 1975 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 337 372 2913 Size: 337 35 2541 Fragments arranged by size: 2541 337 35 DraI TTT'AAA Cuts at: 0 338 2913 Size: 338 2575 DraIII CAC_nnn'GTG Cuts at: 0 1284 2913 Size: 1284 1629 DrdI GACnn_nn'nnGTC Cuts at: 0 798 2913 Size: 798 2115 EaeI y'GGCC_r Cuts at: 0 1811 2033 2913 Size: 1811 222 880 Fragments arranged by size: 1811 880 222 EarI CTCTTCn'nnn_ Cuts at: 0 245 803 1124 1591 2326 2722 2913 Size: 245 558 321 467 735 396 191 Fragments arranged by size: 735 558 467 396 321 245 191 EcoICRI GAG'CTC Cuts at: 0 2319 2913 Size: 2319 594 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 355 748 895 903 1148 1636 2746 Size: 91 264 393 147 8 245 488 1110 Cuts at: 2746 2913 Size: 167 Fragments arranged by size: 1110 488 393 264 245 167 147 91 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 70 2913 Size: 70 2843 EcoO109I rG'GnC_Cy Cuts at: 0 2874 2913 Size: 2874 39 EcoRI G'AATT_C Cuts at: 0 109 488 869 1576 2913 Size: 109 379 381 707 1337 Fragments arranged by size: 1337 707 381 379 109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2791 2913 Size: 2791 122 FspI TGC'GCA Cuts at: 0 934 2913 Size: 934 1979 FspAI rTGC'GCAy Cuts at: 0 934 2913 Size: 934 1979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 464 496 585 617 802 834 940 Size: 29 435 32 89 32 185 32 106 Cuts at: 940 972 1201 1233 1438 1470 1936 1968 2557 Size: 32 229 32 205 32 466 32 589 Cuts at: 2557 2589 2913 Size: 32 324 Fragments arranged by size: 589 466 435 324 229 205 185 106 89 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 501 953 2913 Size: 501 452 1960 Fragments arranged by size: 1960 501 452 HindIII A'AGCT_T Cuts at: 0 524 2913 Size: 524 2389 MfeI C'AATT_G Cuts at: 0 227 1634 2913 Size: 227 1407 1279 Fragments arranged by size: 1407 1279 227 MscI TGG'CCA Cuts at: 0 1813 2035 2913 Size: 1813 222 878 Fragments arranged by size: 1813 878 222 MslI CAynn'nnrTG Cuts at: 0 623 1033 2913 Size: 623 410 1880 Fragments arranged by size: 1880 623 410 MspA1I CmG'CkG Cuts at: 0 190 2913 Size: 190 2723 NcoI C'CATG_G Cuts at: 0 152 411 2030 2913 Size: 152 259 1619 883 Fragments arranged by size: 1619 883 259 152 NsiI A_TGCA'T Cuts at: 0 1804 2913 Size: 1804 1109 NspI r_CATG'y Cuts at: 0 1365 2334 2913 Size: 1365 969 579 Fragments arranged by size: 1365 969 579 PflMI CCAn_nnn'nTGG Cuts at: 0 1820 2434 2913 Size: 1820 614 479 Fragments arranged by size: 1820 614 479 PfoI T'CCnGG_A Cuts at: 0 911 1910 2885 2913 Size: 911 999 975 28 Fragments arranged by size: 999 975 911 28 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2077 2109 2913 Size: 2077 32 804 Fragments arranged by size: 2077 804 32 PpuMI rG'GwC_Cy Cuts at: 0 2874 2913 Size: 2874 39 PvuII CAG'CTG Cuts at: 0 190 2913 Size: 190 2723 SacI G_AGCT'C Cuts at: 0 2321 2913 Size: 2321 592 SapI GCTCTTCn'nnn_ Cuts at: 0 1591 2326 2913 Size: 1591 735 587 Fragments arranged by size: 1591 735 587 SfcI C'TryA_G Cuts at: 0 740 2913 Size: 740 2173 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2041 2913 Size: 2041 872 SmlI C'TyrA_G Cuts at: 0 1152 1551 2248 2913 Size: 1152 399 697 665 Fragments arranged by size: 1152 697 665 399 StuI AGG'CCT Cuts at: 0 1861 2626 2913 Size: 1861 765 287 Fragments arranged by size: 1861 765 287 StyI C'CwwG_G Cuts at: 0 152 411 856 2030 2428 2913 Size: 152 259 445 1174 398 485 Fragments arranged by size: 1174 485 445 398 259 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1663 2913 Size: 1663 1250 TatI w'GTAC_w Cuts at: 0 2060 2169 2333 2913 Size: 2060 109 164 580 Fragments arranged by size: 2060 580 164 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 278 310 965 997 1612 1644 2430 2462 Size: 278 32 655 32 615 32 786 32 Cuts at: 2462 2913 Size: 451 Fragments arranged by size: 786 655 615 451 278 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 2859 2913 Size: 2859 54 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1109 1770 1962 2913 Size: 1109 661 192 951 Fragments arranged by size: 1109 951 661 192 XmnI GAAnn'nnTTC Cuts at: 0 434 2154 2702 2913 Size: 434 1720 548 211 Fragments arranged by size: 1720 548 434 211 Enzymes that do cut: AccI AcuI AflII AhdI AloI AlwNI ApoI BamHI BanI BanII BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI Hin4I HincII HindIII MfeI MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI PpiI PpuMI PvuII SacI SapI SfcI SfiI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BclI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I EagI EciI EcoRV FseI HaeII HpaI KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI XmaI ZraI