################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstXI BstYI Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MamI MflI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 148 GTATAC GT^[AC][GT]AC AccII 81 CGCG CG^CG AciI 80 CCGC CCGC(-3/-1) AlwI 247 GATCC GGATC(4/5) Asp700I 859 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 524 TGGCCA TGG^CCA BanI 630 GGTGCC G^G[CT][GA]CC BanII 75 GAGCCC G[GA]GC[CT]^C BbsI 460 GAAGAC GAAGAC(2/6) BciVI 1305 GTATCC GTATCC(6/5) BcnI 11 CCCGG CC^[GC]GG BfaI 613 CTAG C^TAG BpuAI 460 GAAGAC GAAGAC(2/6) BsaI 29 GAGACC GGTCTC(1/5) BsgI 680 GTGCAG GTGCAG(16/14) BsiEI 138 CGATCG CG[GA][CT]^CG Bsp1407I 110 TGTACA T^GTACA BspMI 160 GCAGGT ACCTGC(4/8) BsrGI 110 TGTACA T^GTACA Bst1107I 148 GTATAC GTA^TAC BstAPI 1251 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 277 GGTTACC G^GT[ACGT]ACC BstPI 277 GGTTACC G^GT[ACGT]ACC BstUI 81 CGCG CG^CG BstZ17I 148 GTATAC GTA^TAC DdeI 1271 CTCAG C^T[ACGT]AG DraII 346 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1171 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 524 TGGCCA [CT]^GGCC[GA] EcoO109I 346 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 277 GGTTACC G^GT[ACGT]ACC EcoRI 487 GAATTC G^AATTC FauI 79 CCCGC CCCGC(4/6) HaeII 581 AGCGCC [GA]GCGC^[CT] HapII 12 CCGG C^CGG HincII 1152 GTCAAC GT[CT]^[GA]AC HindII 1152 GTCAAC GT[CT]^[GA]AC HpaII 12 CCGG C^CGG MaeI 613 CTAG C^TAG MluNI 524 TGGCCA TGG^CCA MscI 524 TGGCCA TGG^CCA MspA1I 356 CAGCTG C[AC]G^C[GT]G MspI 12 CCGG C^CGG MvnI 81 CGCG CG^CG NciI 11 CCCGG CC^[GC]GG PflFI 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 223 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 346 GGGTCCC [GA]G^G[AT]CC[CT] PstI 455 CTGCAG CTGCA^G PvuI 138 CGATCG CGAT^CG PvuII 356 CAGCTG CAG^CTG SfaNI 1239 GATGC GCATC(5/9) SspBI 110 TGTACA T^GTACA SspI 194 AATATT AAT^ATT Tsp45I 1177 GTGAC ^GT[GC]AC Tth111I 50 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 223 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1170 CCACCCTGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 859 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 769 ACATGT A^C[GA][CT]GT 1118 ACATGT AseI 442 ATTAAT AT^TAAT 643 ATTAAT AsnI 442 ATTAAT AT^TAAT 643 ATTAAT BmyI 75 GAGCCC G[AGT]GC[ACT]^C 967 GTGCCC Bsp1286I 75 GAGCCC G[AGT]GC[ACT]^C 967 GTGCCC BspLU11I 769 ACATGT A^CATGT 1118 ACATGT BstNI 229 CCTGG CC^[AT]GG 292 CCTGG DpnII 139 GATC ^GATC 247 GATC EcoNI 398 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 651 CCTGAAGGAGG EcoRII 229 CCTGG ^CC[AT]GG 292 CCTGG EcoT14I 125 CCAAGG C^C[AT][AT]GG 1103 CCAAGG MaeIII 278 GTTAC ^GT[ACGT]AC 1177 GTGAC MboI 139 GATC ^GATC 247 GATC MfeI 4 CAATTG C^AATTG 733 CAATTG MslI 1078 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1224 CACAAACATG MunI 4 CAATTG C^AATTG 733 CAATTG MvaI 229 CCTGG CC^[AT]GG 292 CCTGG NdeII 139 GATC ^GATC 247 GATC PleI 1269 GACTC GAGTC(4/5) 335 GAGTC PshBI 442 ATTAAT AT^TAAT 643 ATTAAT Sau3AI 139 GATC ^GATC 247 GATC SfcI 455 CTGCAG C^T[GA][CT]AG 1292 CTACAG StyI 125 CCAAGG C^C[AT][AT]GG 1103 CCAAGG TaqI 303 TCGA T^CGA 1113 TCGA TthHB8I 303 TCGA T^CGA 1113 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI ApoI AvaII BbvI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I CfoI Cfr13I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MboII MnlI MseI MwoI NlaIII NlaIV NspI RsaI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI