################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 352 AAATTC [GA]^AATT[CT] AfaI 648 GTAC GT^AC Alw44I 258 GTGCAC G^TGCAC AlwI 687 GATCC GGATC(4/5) ApaI 590 GGGCCC GGGCC^C ApaLI 258 GTGCAC G^TGCAC ApoI 352 AAATTC [GA]^AATT[CT] AvaI 97 CCCGAG C^[CT]CG[GA]G BbeI 145 GGCGCC GGCGC^C BpmI 565 CTGGAG CTGGAG(16/14) BseRI 28 GAGGAG GAGGAG(10/8) BsoBI 97 CCCGAG C^[CT]CG[GA]G DpnII 687 GATC ^GATC DraI 349 TTTAAA TTT^AAA EaeI 134 TGGCCG [CT]^GGCC[GA] EcoNI 21 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 458 CCTTGG C^C[AT][AT]GG FseI 678 GGCCGGCC GGCCGG^CC HgaI 93 GACGC GACGC(5/10) KasI 145 GGCGCC G^GCGCC MaeII 284 ACGT A^CGT MboI 687 GATC ^GATC MseI 350 TTAA T^TAA MslI 477 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 145 GGCGCC GG^CGCC NdeII 687 GATC ^GATC PleI 605 GAGTC GAGTC(4/5) PpuMI 745 GGGTCCC [GA]G^G[AT]CC[CT] PstI 598 CTGCAG CTGCA^G PvuII 419 CAGCTG CAG^CTG RsaI 648 GTAC GT^AC SacI 190 GAGCTC GAGCT^C Sau3AI 687 GATC ^GATC ScaI 647 AGTACT AGT^ACT SfaNI 380 GCATC GCATC(5/9) SfcI 598 CTGCAG C^T[GA][CT]AG SfiI 535 GGCCTCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 145 GGCGCC GGC^GCC StuI 543 AGGCCT AGG^CCT StyI 458 CCTTGG C^C[AT][AT]GG TfiI 411 GAATC G^A[AT]TC Tru9I 350 TTAA T^TAA Tsp509I 353 AATT ^AATT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcyI 93 GACGCC G[GA]^CG[CT]C 145 GGCGCC BanII 190 GAGCTC G[GA]GC[CT]^C 590 GGGCCC BsaHI 93 GACGCC G[GA]^CG[CT]C 145 GGCGCC BsgI 339 CTGCAC GTGCAG(16/14) 497 GTGCAG BsmI 372 GAATGC GAATGC(1/-1) 579 GCATTC BspMI 304 ACCTGC ACCTGC(4/8) 80 GCAGGT DdeI 487 CTCAG C^T[ACGT]AG 540 CTCAG Hin1I 93 GACGCC G[GA]^CG[CT]C 145 GGCGCC HinfI 411 GAATC G^A[ACGT]TC 605 GAGTC KspI 50 CCGCGG CCGC^GG 314 CCGCGG MboII 17 TCTTC GAAGA(8/7) 356 TCTTC NlaIII 453 CATG CATG^ 480 CATG SacII 50 CCGCGG CCGC^GG 314 CCGCGG TspRI 434 CAGTG CA[GC]TG[ACGT][ACGT]^ 725 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BanI BbvI BcnI BglI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV Sau96I ScrFI TseI