################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BcgI BcnI BglII BlnI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MluI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 385 GTATAC GT^[AC][GT]AC AccII 859 CGCG CG^CG AccIII 461 TCCGGA T^CCGGA AcsI 1223 AAATTC [GA]^AATT[CT] AflII 2047 CTTAAG C^TTAAG AocI 273 CCTGAGG CC^T[ACGT]AGG ApoI 1223 AAATTC [GA]^AATT[CT] AvaI 105 CTCGGG C^[CT]CG[GA]G BanI 1174 GGCACC G^G[CT][GA]CC BbrPI 1183 CACGTG CAC^GTG BbsI 280 GAAGAC GAAGAC(2/6) BciVI 585 GGATAC GTATCC(6/5) BclI 422 TGATCA T^GATCA BfrI 2047 CTTAAG C^TTAAG BglI 1735 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 280 GAAGAC GAAGAC(2/6) BsaAI 1183 CACGTG [CT]AC^GT[GA] BseAI 461 TCCGGA T^CCGGA BsmI 1928 GCATTC GAATGC(1/-1) BsoBI 105 CTCGGG C^[CT]CG[GA]G BspEI 461 TCCGGA T^CCGGA BspHI 1415 TCATGA T^CATGA BspMI 966 GCAGGT ACCTGC(4/8) BsrBI 928 GAGCGG CCGCTC(-3/-3) BsrDI 1265 CATTGC GCAATG(2/0) BsrFI 1670 ACCGGC [GA]^CCGG[CT] Bst1107I 385 GTATAC GTA^TAC BstUI 859 CGCG CG^CG BstZ17I 385 GTATAC GTA^TAC Bsu36I 273 CCTGAGG CC^T[ACGT]AGG Cfr10I 1670 ACCGGC [GA]^CCGG[CT] DraIII 1811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 273 CCTGAGG CC^T[ACGT]AGG FbaI 422 TGATCA T^GATCA HgaI 11 GCGTC GACGC(5/10) HincII 1324 GTCAAC GT[CT]^[GA]AC HindII 1324 GTCAAC GT[CT]^[GA]AC MfeI 390 CAATTG C^AATTG MroI 461 TCCGGA T^CCGGA MunI 390 CAATTG C^AATTG MvnI 859 CGCG CG^CG NdeI 428 CATATG CA^TATG PmaCI 1183 CACGTG CAC^GTG PmlI 1183 CACGTG CAC^GTG PpuMI 353 AGGACCT [GA]G^G[AT]CC[CT] PshAI 547 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1415 TCATGA T^CATGA SapI 1196 GCTCTTC GCTCTTC(1/4) SexAI 1812 ACCTGGT A^CC[AT]GGT SspI 1079 AATATT AAT^ATT StuI 977 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 1302 GATCC GGATC(4/5) 1531 GGATC BanII 1797 GAGCCC G[GA]GC[CT]^C 1901 GAGCCC BfaI 1003 CTAG C^TAG 1478 CTAG BsaBI 1210 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1296 GATCGAGATC BsaI 454 GAGACC GGTCTC(1/5) 1697 GGTCTC BsaWI 461 TCCGGA [AT]^CCGG[AT] 548 ACCGGA BstAPI 561 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 765 GCAGAGTCTGC BstXI 1373 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1935 CCACCTGGCTGG BstYI 1301 AGATCC [GA]^GATC[CT] 1531 GGATCT DraII 353 AGGACCT [GA]G^G[ACGT]CC[CT] 1034 AGGCCCT EarI 1197 CTCTTC CTCTTC(1/4) 1155 GAAGAG EcoNI 48 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 357 CCTGTGGAAGG EcoO109I 353 AGGACCT [GA]G^G[ACGT]CC[CT] 1034 AGGCCCT FauI 909 GCGGG CCCGC(4/6) 1269 GCGGG Ksp632I 1197 CTCTTC CTCTTC(1/4) 1155 GAAGAG MaeI 1003 CTAG C^TAG 1478 CTAG MaeII 1184 ACGT A^CGT 1427 ACGT MamI 1210 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1296 GATCGAGATC MflI 1301 AGATCC [GA]^GATC[CT] 1531 GGATCT MseI 2036 TTAA T^TAA 2048 TTAA NcoI 324 CCATGG C^CATGG 1761 CCATGG SfaNI 232 GATGC GCATC(5/9) 1337 GCATC Tru9I 2036 TTAA T^TAA 2048 TTAA XcmI 236 CCAATGGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 488 CCATGCCAAGGCTGG XhoII 1301 AGATCC [GA]^GATC[CT] 1531 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BalI BbvCI BbvI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I