(Circular) (Six-base) MAPSORT of: pFN21AE4561.seq Check: 1836 from: 1 to: 6760 pFN21AE4561 6760 bp With 182 enzymes: SgfI * March 28, 2012 14:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4570 278 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 4103 1937 Size: 454 1712 4594 Fragments arranged by size: 4594 1712 454 Acc65I G'GTAC_C Cuts at: 4081 4081 Size: 6760 AclI AA'CG_TT Cuts at: 1563 4887 5260 1563 Size: 3324 373 3063 Fragments arranged by size: 3324 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 2600 2771 2924 3029 4823 6207 6475 Size: 929 39 171 153 105 1794 1384 268 Cuts at: 6475 1632 Size: 1917 Fragments arranged by size: 1917 1794 1384 929 268 171 153 105 39 AflII C'TTAA_G Cuts at: 820 1017 4052 820 Size: 197 3035 3528 Fragments arranged by size: 3528 3035 197 AflIII A'CryG_T Cuts at: 1203 5659 6636 1203 Size: 4456 977 1327 Fragments arranged by size: 4456 1327 977 AgeI A'CCGG_T Cuts at: 5591 5591 Size: 6760 AhdI GACnn_n'nnGTC Cuts at: 5489 5489 Size: 6760 AleI CACnn'nnGTG Cuts at: 1166 2435 3585 1166 Size: 1269 1150 4341 Fragments arranged by size: 4341 1269 1150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2164 1184 Size: 980 5780 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 2696 Size: 32 6728 AlwNI CAG_nnn'CTG Cuts at: 2425 2534 2572 2776 2902 3124 3511 4503 6075 Size: 109 38 204 126 222 387 992 1572 Cuts at: 6075 2425 Size: 3110 Fragments arranged by size: 3110 1572 992 387 222 204 126 109 38 ApaLI G'TGCA_C Cuts at: 4817 5973 4817 Size: 1156 5604 ApoI r'AATT_y Cuts at: 1493 3228 4069 4295 4447 6556 1493 Size: 1735 841 226 152 2109 1697 Fragments arranged by size: 2109 1735 1697 841 226 152 AseI AT'TA_AT Cuts at: 160 1032 5314 160 Size: 872 4282 1606 Fragments arranged by size: 4282 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 AvaI C'yCGr_G Cuts at: 1870 1943 2110 4085 1870 Size: 73 167 1975 4545 Fragments arranged by size: 4545 1975 167 73 BamHI G'GATC_C Cuts at: 4090 4457 4090 Size: 367 6393 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3179 4081 5536 618 Size: 325 217 627 1392 902 1455 1842 Fragments arranged by size: 1842 1455 1392 902 627 325 217 BanII G_rGCy'C Cuts at: 1391 3806 3910 4079 1391 Size: 2415 104 169 4072 Fragments arranged by size: 4072 2415 169 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 5396 Fragments arranged by size: 5396 971 393 BbvCI CC'TCA_GC Cuts at: 2072 2955 3808 3966 2072 Size: 883 853 158 4866 Fragments arranged by size: 4866 883 853 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4951 4985 1903 Size: 34 3014 34 3678 Fragments arranged by size: 3678 3014 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2584 4652 5862 2584 Size: 2068 1210 3482 Fragments arranged by size: 3482 2068 1210 BclI T'GATC_A Cuts at: 1548 2427 1548 Size: 879 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3746 5371 136 Size: 107 122 71 631 605 2074 1625 1525 Fragments arranged by size: 2074 1625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6755 6755 Size: 6760 BlpI GC'TnA_GC Cuts at: 2766 2883 3240 3800 4177 2766 Size: 117 357 560 377 5349 Fragments arranged by size: 5349 560 377 357 117 Bme1580I G_kGCm'C Cuts at: 1790 4821 5977 1790 Size: 3031 1156 2573 Fragments arranged by size: 3031 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3433 1318 Size: 2115 4645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4491 5449 442 Size: 416 53 83 399 24 3074 958 1753 Fragments arranged by size: 3074 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3018 3873 3988 5420 1239 Size: 1779 855 115 1432 2579 Fragments arranged by size: 2579 1779 1432 855 115 Bpu10I CC'TnA_GC Cuts at: 2072 2955 3808 3966 2072 Size: 883 853 158 4866 Fragments arranged by size: 4866 883 853 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 3007 4885 5750 6048 2621 Size: 386 1878 865 298 3333 Fragments arranged by size: 3333 1878 865 386 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 3708 5423 882 Size: 617 954 1255 1715 2219 Fragments arranged by size: 2219 1715 1255 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3190 4551 493 Size: 711 1986 1361 2702 Fragments arranged by size: 2702 1986 1361 711 BsaBI GATnn'nnATC Cuts at: 3219 3305 6754 3219 Size: 86 3449 3225 Fragments arranged by size: 3449 3225 86 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4567 4949 5584 275 Size: 53 83 186 936 3034 382 635 1451 Fragments arranged by size: 3034 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2466 2553 5193 5591 5865 6012 6423 2466 Size: 87 2640 398 274 147 411 2803 Fragments arranged by size: 2803 2640 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 2696 2726 3443 Size: 30 165 30 945 30 318 30 717 Cuts at: 3443 3473 3871 3901 1178 Size: 30 398 30 4037 Fragments arranged by size: 4037 945 717 398 318 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2039 2141 2471 2601 2828 2972 2975 Size: 841 12 102 330 130 227 144 3 Cuts at: 2975 3464 3606 3983 1186 Size: 489 142 377 3963 Fragments arranged by size: 3963 841 489 377 330 227 144 142 130 102 12 3 BseYI C'CCAG_C Cuts at: 2895 3377 3860 5963 6459 2895 Size: 482 483 2103 496 3196 Fragments arranged by size: 3196 2103 496 483 482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2783 3269 1163 Size: 573 303 744 486 4654 Fragments arranged by size: 4654 744 573 486 303 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4210 4971 5999 1540 Size: 275 186 2209 761 1028 2301 Fragments arranged by size: 2301 2209 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 2724 2820 4821 4906 5977 2601 Size: 123 96 2001 85 1071 3384 Fragments arranged by size: 3384 2001 1071 123 96 85 BsmI GAATG_Cn' Cuts at: 3933 4271 4364 3933 Size: 338 93 6329 Fragments arranged by size: 6329 338 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2601 2724 2820 3806 3910 4079 4821 Size: 399 811 123 96 986 104 169 742 Cuts at: 4821 4906 5977 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 986 811 742 399 169 123 104 96 85 BspEI T'CCGG_A Cuts at: 2466 6423 2466 Size: 3957 2803 BspHI T'CATG_A Cuts at: 3420 4649 6379 3420 Size: 1229 1730 3801 Fragments arranged by size: 3801 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2962 4115 844 Size: 2118 1153 3489 Fragments arranged by size: 3489 2118 1153 BsrBI CCG'CTC Cuts at: 1394 1823 2935 4647 1394 Size: 429 1112 1712 3507 Fragments arranged by size: 3507 1712 1112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3269 5255 5429 65 Size: 1183 2021 1986 174 1396 Fragments arranged by size: 2021 1986 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3675 5404 5591 1700 Size: 110 1865 1729 187 2869 Fragments arranged by size: 2869 1865 1729 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6760 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6760 BssSI C'ACGA_G Cuts at: 4820 5832 4820 Size: 1012 5748 BstAPI GCAn_nnn'nTGC Cuts at: 794 2572 2776 794 Size: 1778 204 4778 Fragments arranged by size: 4778 1778 204 BstBI TT'CG_AA Cuts at: 5564 5564 Size: 6760 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6760 BstXI CCAn_nnnn'nTGG Cuts at: 3385 3947 3385 Size: 562 6198 BstYI r'GATC_y Cuts at: 1972 3306 3536 4090 4457 4842 4859 6311 6397 Size: 1334 230 554 367 385 17 1452 86 Cuts at: 6397 6409 6755 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1334 554 385 367 346 230 86 17 12 BstZ17I GTA'TAC Cuts at: 2392 2392 Size: 6760 Bsu36I CC'TnA_GG Cuts at: 2279 2279 Size: 6760 BtgI C'CryG_G Cuts at: 513 1065 2004 2329 2403 3766 513 Size: 552 939 325 74 1363 3507 Fragments arranged by size: 3507 1363 939 552 325 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2880 487 Size: 683 56 1654 4367 Fragments arranged by size: 4367 1654 683 56 BtsI GCAGTG_nn' Cuts at: 2133 3502 4276 5070 5090 2133 Size: 1369 774 794 20 3803 Fragments arranged by size: 3803 1369 794 774 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6760 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6725 DraI TTT'AAA Cuts at: 4064 4064 Size: 6760 DraIII CAC_nnn'GTG Cuts at: 3821 3821 Size: 6760 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5767 809 Size: 507 4451 1802 Fragments arranged by size: 4451 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2485 3129 3738 Size: 54 1425 50 275 18 655 644 609 Cuts at: 3738 4207 5096 6725 8 Size: 469 889 1629 43 Fragments arranged by size: 1629 1425 889 655 644 609 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4207 1537 Size: 275 2395 4090 Fragments arranged by size: 4090 2395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3155 3208 4690 1353 Size: 1802 53 1482 3423 Fragments arranged by size: 3423 1802 1482 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5347 5721 5867 387 Size: 1064 138 3758 374 146 1280 Fragments arranged by size: 3758 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2561 2600 2771 2924 3018 3029 3873 Size: 393 929 39 171 153 94 11 844 Cuts at: 3873 3988 4823 5420 6207 6475 1239 Size: 115 835 597 787 268 1524 Fragments arranged by size: 1524 929 844 835 787 597 393 268 171 153 115 94 39 11 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2366 2057 Size: 309 6451 EcoO109I rG'GnC_Cy Cuts at: 2359 3040 2359 Size: 681 6079 EcoRI G'AATT_C Cuts at: 4069 4447 4069 Size: 378 6382 FspI TGC'GCA Cuts at: 5266 5266 Size: 6760 HaeII r_GCGC'y Cuts at: 1678 3283 3511 3761 5907 1678 Size: 1605 228 250 2146 2531 Fragments arranged by size: 2531 2146 1605 250 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2996 3028 3443 3445 3475 Size: 32 135 32 1424 32 415 2 30 Cuts at: 3475 3477 3554 3586 3869 3901 3965 3997 5401 Size: 2 77 32 283 32 64 32 1404 Cuts at: 5401 5433 5475 5507 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1424 1404 415 283 135 77 64 42 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3331 4104 4350 669 Size: 988 281 1393 773 246 3079 Fragments arranged by size: 3079 1393 988 773 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6760 HpaI GTT'AAC Cuts at: 4350 4350 Size: 6760 KpnI G_GTAC'C Cuts at: 4085 4085 Size: 6760 MfeI C'AATT_G Cuts at: 2395 4359 2395 Size: 1964 4796 MluI A'CGCG_T Cuts at: 6636 6636 Size: 6760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2946 5874 6058 1305 Size: 1641 2928 184 2007 Fragments arranged by size: 2928 2007 1641 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2487 3131 3740 10 Size: 54 1425 343 655 644 609 3030 Fragments arranged by size: 3030 1425 655 644 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2435 2522 2651 3585 4718 5077 Size: 648 1185 84 87 129 934 1133 359 Cuts at: 5077 5236 518 Size: 159 2042 Fragments arranged by size: 2042 1185 1133 934 648 359 159 129 87 84 MspA1I CmG'CkG Cuts at: 1606 2578 2899 3061 3121 3291 3381 4176 4853 Size: 972 321 162 60 170 90 795 677 Cuts at: 4853 6001 6246 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 972 795 677 321 245 170 162 90 60 NcoI C'CATG_G Cuts at: 513 1065 2004 2329 3766 513 Size: 552 939 325 1437 3507 Fragments arranged by size: 3507 1437 939 552 325 NdeI CA'TA_TG Cuts at: 387 2434 387 Size: 2047 4713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6760 NotI GC'GGCC_GC Cuts at: 4207 4207 Size: 6760 NspI r_CATG'y Cuts at: 1132 1229 4118 5663 1132 Size: 97 2889 1545 2229 Fragments arranged by size: 2889 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3698 1348 Size: 2350 4410 PciI A'CATG_T Cuts at: 5659 5659 Size: 6760 PflMI CCAn_nnn'nTGG Cuts at: 1108 3352 3527 3601 3772 3821 1108 Size: 2244 175 74 171 49 4047 Fragments arranged by size: 4047 2244 175 171 74 49 PmeI GTTT'AAAC Cuts at: 4064 4064 Size: 6760 PmlI CAC'GTG Cuts at: 3190 3190 Size: 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2728 4829 4861 6342 6374 2696 Size: 32 2101 32 1481 32 3082 Fragments arranged by size: 3082 2101 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 6760 PshAI GACnn'nnGTC Cuts at: 1613 2556 1613 Size: 943 5817 PsiI TTA'TAA Cuts at: 4330 4330 Size: 6760 PstI C_TGCA'G Cuts at: 830 2140 2478 2577 3060 3492 3637 4112 830 Size: 1310 338 99 483 432 145 475 3478 Fragments arranged by size: 3478 1310 483 475 432 338 145 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6760 PvuII CAG'CTG Cuts at: 2578 3061 3121 3381 2578 Size: 483 60 260 5957 Fragments arranged by size: 5957 483 260 60 SalI G'TCGA_C Cuts at: 1936 4102 1936 Size: 2166 4594 SapI GCTCTTCn'nnn_ Cuts at: 3208 3208 Size: 6760 SbfI CC_TGCA'GG Cuts at: 4112 4112 Size: 6760 ScaI AGT'ACT Cuts at: 5008 6720 5008 Size: 1712 5048 SexAI A'CCwGG_T Cuts at: 3817 3817 Size: 6760 SfcI C'TryA_G Cuts at: 826 1045 2136 2474 2573 3056 3488 3633 4108 Size: 219 1091 338 99 483 432 145 475 Cuts at: 4108 5243 5924 6115 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1091 681 483 475 432 338 219 191 145 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6760 SmaI CCC'GGG Cuts at: 1872 4087 1872 Size: 2215 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2636 2986 4052 4864 5765 6027 Size: 197 926 693 350 1066 812 901 262 Cuts at: 6027 820 Size: 1553 Fragments arranged by size: 1553 1066 926 901 812 693 350 262 197 SnaBI TAC'GTA Cuts at: 493 4551 493 Size: 4058 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6760 SphI G_CATG'C Cuts at: 1132 4118 1132 Size: 2986 3774 SspI AAT'ATT Cuts at: 5 52 3086 4684 5 Size: 47 3034 1598 2081 Fragments arranged by size: 3034 2081 1598 47 StuI AGG'CCT Cuts at: 1517 2984 1517 Size: 1467 5293 StyI C'CwwG_G Cuts at: 513 1065 2004 2329 2498 2715 3132 3766 4199 Size: 552 939 325 169 217 417 634 433 Cuts at: 4199 513 Size: 3074 Fragments arranged by size: 3074 939 634 552 433 417 325 217 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3711 4787 4804 4957 5142 5587 325 Size: 1255 2131 1076 17 153 185 445 1498 Fragments arranged by size: 2131 1498 1255 1076 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3392 5006 6718 Size: 274 80 33 51 1443 1415 1614 1712 Cuts at: 6718 96 Size: 138 Fragments arranged by size: 1712 1614 1443 1415 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 3188 3220 3871 3903 3919 3951 6720 Size: 32 808 32 651 32 16 32 2769 Cuts at: 6720 6752 2348 Size: 32 2356 Fragments arranged by size: 2769 2356 808 651 32 32 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6760 XbaI T'CTAG_A Cuts at: 4096 4096 Size: 6760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2500 2248 Size: 252 6508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6760 XmaI C'CCGG_G Cuts at: 1870 4085 1870 Size: 2215 4545 XmnI GAAnn'nnTTC Cuts at: 4889 4889 Size: 6760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4568 276 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI