(Linear) (Six-base) MAPSORT of: KIEE4561.seq Check: 6540 from: 1 to: 2055 KIEE4561.orf With 182 enzymes: SgfI * March 22, 2012 18:23 .. AccI GT'mk_AC Cuts at: 0 386 2055 Size: 386 1669 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 595 766 919 1024 2055 Size: 556 39 171 153 105 1031 Fragments arranged by size: 1031 556 171 153 105 39 AflII C'TTAA_G Cuts at: 0 2047 2055 Size: 2047 8 AleI CACnn'nnGTG Cuts at: 0 430 1580 2055 Size: 430 1150 475 Fragments arranged by size: 1150 475 430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 159 2055 Size: 159 1896 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 691 723 2055 Size: 691 32 1332 Fragments arranged by size: 1332 691 32 AlwNI CAG_nnn'CTG Cuts at: 0 420 529 567 771 897 1119 1506 2055 Size: 420 109 38 204 126 222 387 549 Fragments arranged by size: 549 420 387 222 204 126 109 38 ApoI r'AATT_y Cuts at: 0 1223 2055 Size: 1223 832 AvaI C'yCGr_G Cuts at: 0 105 2055 Size: 105 1950 BanI G'GyrC_C Cuts at: 0 1174 2055 Size: 1174 881 BanII G_rGCy'C Cuts at: 0 1801 1905 2055 Size: 1801 104 150 Fragments arranged by size: 1801 150 104 BbsI GAAGACnn'nnnn_ Cuts at: 0 287 2055 Size: 287 1768 BbvCI CC'TCA_GC Cuts at: 0 67 950 1803 1961 2055 Size: 67 883 853 158 94 Fragments arranged by size: 883 853 158 94 67 BciVI GTATCCnnnnn_n' Cuts at: 0 579 2055 Size: 579 1476 BclI T'GATC_A Cuts at: 0 422 2055 Size: 422 1633 BglI GCCn_nnn'nGGC Cuts at: 0 1741 2055 Size: 1741 314 BlpI GC'TnA_GC Cuts at: 0 761 878 1235 1795 2055 Size: 761 117 357 560 260 Fragments arranged by size: 761 560 357 260 117 BmgBI CAC'GTC Cuts at: 0 1428 2055 Size: 1428 627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1013 1868 1983 2055 Size: 1013 855 115 72 Fragments arranged by size: 1013 855 115 72 Bpu10I CC'TnA_GC Cuts at: 0 67 950 1803 1961 2055 Size: 67 883 853 158 94 Fragments arranged by size: 883 853 158 94 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 1002 2055 Size: 616 386 1053 Fragments arranged by size: 1053 616 386 BsaI GGTCTCn'nnnn_ Cuts at: 0 448 1703 2055 Size: 448 1255 352 Fragments arranged by size: 1255 448 352 BsaAI yAC'GTr Cuts at: 0 1185 2055 Size: 1185 870 BsaBI GATnn'nnATC Cuts at: 0 1214 1300 2055 Size: 1214 86 755 Fragments arranged by size: 1214 755 86 BsaWI w'CCGG_w Cuts at: 0 461 548 2055 Size: 461 87 1507 Fragments arranged by size: 1507 461 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 343 373 691 721 1438 1468 1866 1896 Size: 343 30 318 30 717 30 398 30 Cuts at: 1896 2055 Size: 159 Fragments arranged by size: 717 398 343 318 159 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 34 136 466 596 823 967 970 Size: 22 12 102 330 130 227 144 3 Cuts at: 970 1459 1601 1978 2055 Size: 489 142 377 77 Fragments arranged by size: 489 377 330 227 144 142 130 102 77 22 12 3 BseYI C'CCAG_C Cuts at: 0 890 1372 1855 2055 Size: 890 482 483 200 Fragments arranged by size: 890 483 482 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 778 1264 2055 Size: 34 744 486 791 Fragments arranged by size: 791 744 486 34 BsiHKAI G_wGCw'C Cuts at: 0 596 719 815 2055 Size: 596 123 96 1240 Fragments arranged by size: 1240 596 123 96 BsmI GAATG_Cn' Cuts at: 0 1928 2055 Size: 1928 127 Bsp1286I G_dGCh'C Cuts at: 0 596 719 815 1801 1905 2055 Size: 596 123 96 986 104 150 Fragments arranged by size: 986 596 150 123 104 96 BspEI T'CCGG_A Cuts at: 0 461 2055 Size: 461 1594 BspHI T'CATG_A Cuts at: 0 1415 2055 Size: 1415 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 957 2055 Size: 957 1098 BsrBI CCG'CTC Cuts at: 0 930 2055 Size: 930 1125 BsrDI GCAATG_nn' Cuts at: 0 1264 2055 Size: 1264 791 BsrFI r'CCGG_y Cuts at: 0 1670 2055 Size: 1670 385 BstAPI GCAn_nnn'nTGC Cuts at: 0 567 771 2055 Size: 567 204 1284 Fragments arranged by size: 1284 567 204 BstXI CCAn_nnnn'nTGG Cuts at: 0 1380 1942 2055 Size: 1380 562 113 Fragments arranged by size: 1380 562 113 BstYI r'GATC_y Cuts at: 0 1301 1531 2055 Size: 1301 230 524 Fragments arranged by size: 1301 524 230 BstZ17I GTA'TAC Cuts at: 0 387 2055 Size: 387 1668 Bsu36I CC'TnA_GG Cuts at: 0 274 2055 Size: 274 1781 BtgI C'CryG_G Cuts at: 0 324 398 1761 2055 Size: 324 74 1363 294 Fragments arranged by size: 1363 324 294 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 875 2055 Size: 875 1180 BtsI GCAGTG_nn' Cuts at: 0 128 1497 2055 Size: 128 1369 558 Fragments arranged by size: 1369 558 128 DraIII CAC_nnn'GTG Cuts at: 0 1816 2055 Size: 1816 239 EaeI y'GGCC_r Cuts at: 0 480 1124 1733 2055 Size: 480 644 609 322 Fragments arranged by size: 644 609 480 322 EarI CTCTTCn'nnn_ Cuts at: 0 1150 1203 2055 Size: 1150 53 852 Fragments arranged by size: 1150 852 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 595 766 919 1013 1024 1868 1983 Size: 556 39 171 153 94 11 844 115 Cuts at: 1983 2055 Size: 72 Fragments arranged by size: 844 556 171 153 115 94 72 39 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 361 2055 Size: 52 309 1694 Fragments arranged by size: 1694 309 52 EcoO109I rG'GnC_Cy Cuts at: 0 354 1035 2055 Size: 354 681 1020 Fragments arranged by size: 1020 681 354 HaeII r_GCGC'y Cuts at: 0 1278 1506 1756 2055 Size: 1278 228 250 299 Fragments arranged by size: 1278 299 250 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 991 1023 1438 1440 1470 1472 1549 1581 Size: 991 32 415 2 30 2 77 32 Cuts at: 1581 1864 1896 1960 1992 2055 Size: 283 32 64 32 63 Fragments arranged by size: 991 415 283 77 64 63 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 0 1326 2055 Size: 1326 729 MfeI C'AATT_G Cuts at: 0 390 2055 Size: 390 1665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 941 2055 Size: 941 1114 MscI TGG'CCA Cuts at: 0 482 1126 1735 2055 Size: 482 644 609 320 Fragments arranged by size: 644 609 482 320 MslI CAynn'nnrTG Cuts at: 0 346 430 517 646 1580 2055 Size: 346 84 87 129 934 475 Fragments arranged by size: 934 475 346 129 87 84 MspA1I CmG'CkG Cuts at: 0 573 894 1056 1116 1286 1376 2055 Size: 573 321 162 60 170 90 679 Fragments arranged by size: 679 573 321 170 162 90 60 NcoI C'CATG_G Cuts at: 0 324 1761 2055 Size: 324 1437 294 Fragments arranged by size: 1437 324 294 NdeI CA'TA_TG Cuts at: 0 429 2055 Size: 429 1626 PasI CC'CwG_GG Cuts at: 0 1693 2055 Size: 1693 362 PflMI CCAn_nnn'nTGG Cuts at: 0 1347 1522 1596 1767 1816 2055 Size: 1347 175 74 171 49 239 Fragments arranged by size: 1347 239 175 171 74 49 PmlI CAC'GTG Cuts at: 0 1185 2055 Size: 1185 870 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 691 723 2055 Size: 691 32 1332 Fragments arranged by size: 1332 691 32 PpuMI rG'GwC_Cy Cuts at: 0 354 2055 Size: 354 1701 PshAI GACnn'nnGTC Cuts at: 0 551 2055 Size: 551 1504 PstI C_TGCA'G Cuts at: 0 135 473 572 1055 1487 1632 2055 Size: 135 338 99 483 432 145 423 Fragments arranged by size: 483 432 423 338 145 135 99 PvuII CAG'CTG Cuts at: 0 573 1056 1116 1376 2055 Size: 573 483 60 260 679 Fragments arranged by size: 679 573 483 260 60 SapI GCTCTTCn'nnn_ Cuts at: 0 1203 2055 Size: 1203 852 SexAI A'CCwGG_T Cuts at: 0 1812 2055 Size: 1812 243 SfcI C'TryA_G Cuts at: 0 131 469 568 1051 1483 1628 2055 Size: 131 338 99 483 432 145 427 Fragments arranged by size: 483 432 427 338 145 131 99 SmlI C'TyrA_G Cuts at: 0 631 981 2047 2055 Size: 631 350 1066 8 Fragments arranged by size: 1066 631 350 8 SspI AAT'ATT Cuts at: 0 1081 2055 Size: 1081 974 StuI AGG'CCT Cuts at: 0 979 2055 Size: 979 1076 StyI C'CwwG_G Cuts at: 0 324 493 710 1127 1761 2055 Size: 324 169 217 417 634 294 Fragments arranged by size: 634 417 324 294 217 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1706 2055 Size: 1706 349 TatI w'GTAC_w Cuts at: 0 1387 2055 Size: 1387 668 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 343 375 1183 1215 1866 1898 1914 1946 Size: 343 32 808 32 651 32 16 32 Cuts at: 1946 2055 Size: 109 Fragments arranged by size: 808 651 343 109 32 32 32 32 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 243 495 2055 Size: 243 252 1560 Fragments arranged by size: 1560 252 243 Enzymes that do cut: AccI AcuI AflII AleI AlfI AloI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BglI BlpI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NdeI PasI PflMI PmlI PpiI PpuMI PshAI PstI PvuII SapI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglII Bme1580I BmrI BmtI BplI BsaHI BsiEI BsiWI BsmBI BsrGI BssHII BssSI BstBI BstEII ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI