################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BbrPI BcgI BclI BfrI BglI BglII BlnI BsaAI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI KpnI MfeI MluI MluNI MscI MunI NaeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1405 GACGTC GACGT^C AccIII 1204 TCCGGA T^CCGGA AlwNI 94 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1765 CCTCAGG CC^T[ACGT]AGG ApaI 1153 GGGCCC GGGCC^C AspI 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 587 GGATCC G^GATCC BbeI 1315 GGCGCC GGCGC^C BbsI 857 GAAGAC GAAGAC(2/6) BciVI 1207 GGATAC GTATCC(6/5) BlpI 414 GCTAAGC GC^T[ACGT]AGC Bpu1102I 414 GCTAAGC GC^T[ACGT]AGC BpuAI 857 GAAGAC GAAGAC(2/6) BseAI 1204 TCCGGA T^CCGGA BseRI 266 CTCCTC GAGGAG(10/8) Bsp1407I 1731 TGTACA T^GTACA BspEI 1204 TCCGGA T^CCGGA BspMI 272 ACCTGC ACCTGC(4/8) BsrGI 1731 TGTACA T^GTACA BssSI 1634 CTCGTG CACGAG(-5/-1) BstBI 254 TTCGAA TT^CGAA Bsu36I 1765 CCTCAGG CC^T[ACGT]AGG CelII 414 GCTAAGC GC^T[ACGT]AGC EaeI 1560 TGGCCG [CT]^GGCC[GA] Eco81I 1765 CCTCAGG CC^T[ACGT]AGG EcoNI 1390 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 679 GATATC GAT^ATC EcoT14I 404 CCATGG C^C[AT][AT]GG HaeII 1315 GGCGCC [GA]GCGC^[CT] HindIII 7 AAGCTT A^AGCTT HpaI 1063 GTTAAC GTT^AAC KasI 1315 GGCGCC G^GCGCC KspI 1158 CCGCGG CCGC^GG MroI 1204 TCCGGA T^CCGGA MslI 969 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1315 GGCGCC GG^CGCC NcoI 404 CCATGG C^CATGG NdeI 374 CATATG CA^TATG NheI 1696 GCTAGC G^CTAGC NspI 757 GCATGC [GA]CATG^[CT] NspV 254 TTCGAA TT^CGAA PflFI 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1305 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1772 GAGTC GAGTC(4/5) PpuMI 95 AGGTCCT [GA]G^G[AT]CC[CT] SacII 1158 CCGCGG CCGC^GG SfoI 1315 GGCGCC GGC^GCC SfuI 254 TTCGAA TT^CGAA SphI 757 GCATGC GCATG^C SspBI 1731 TGTACA T^GTACA StyI 404 CCATGG C^C[AT][AT]GG Tth111I 663 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1305 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1230 CCATCACCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccII 154 CGCG CG^CG 1159 CGCG AcsI 245 AAATTC [GA]^AATT[CT] 882 AAATTT AcyI 1315 GGCGCC G[GA]^CG[CT]C 1405 GACGTC AfaI 1224 GTAC GT^AC 1732 GTAC ApoI 245 AAATTC [GA]^AATT[CT] 882 AAATTT Asp700I 814 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1287 GAATCTCTTC AspHI 477 GAGCTC G[AT]GC[AT]^C 1163 GAGCTC BanI 613 GGTGCC G^G[CT][GA]CC 1315 GGCGCC BbvCI 493 CCTCAGC CCTCAGC(-5/-2) 1098 CCTCAGC BfaI 808 CTAG C^TAG 1697 CTAG BpmI 1215 CTCCAG CTGGAG(16/14) 701 CTGGAG BsaBI 918 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1419 GATGGTCATC BsaHI 1315 GGCGCC G[GA]^CG[CT]C 1405 GACGTC BsaI 163 GAGACC GGTCTC(1/5) 799 GAGACC BsaWI 1204 TCCGGA [AT]^CCGG[AT] 1440 ACCGGA BsgI 1706 CTGCAC GTGCAG(16/14) 1616 GTGCAG BsiHKAI 477 GAGCTC G[AT]GC[AT]^C 1163 GAGCTC BstUI 154 CGCG CG^CG 1159 CGCG BstXI 537 CCACGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 603 CCAGTTTGTTGG BstYI 587 GGATCC [GA]^GATC[CT] 1469 GGATCT DsaI 404 CCATGG C^C[GA][CT]GG 1158 CCGCGG Hin1I 1315 GGCGCC G[GA]^CG[CT]C 1405 GACGTC MaeI 808 CTAG C^TAG 1697 CTAG MaeII 368 ACGT A^CGT 1406 ACGT MamI 918 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1419 GATGGTCATC MflI 587 GGATCC [GA]^GATC[CT] 1469 GGATCT MvnI 154 CGCG CG^CG 1159 CGCG PvuII 1351 CAGCTG CAG^CTG 1556 CAGCTG RsaI 1224 GTAC GT^AC 1732 GTAC SacI 477 GAGCTC GAGCT^C 1163 GAGCTC SmaI 524 CCCGGG CCC^GGG 671 CCCGGG StuI 992 AGGCCT AGG^CCT 1037 AGGCCT TaqI 161 TCGA T^CGA 255 TCGA TthHB8I 161 TCGA T^CGA 255 TCGA XhoII 587 GGATCC [GA]^GATC[CT] 1469 GGATCT XmaCI 524 CCCGGG C^CCGGG 671 CCCGGG XmaI 524 CCCGGG C^CCGGG 671 CCCGGG XmnI 814 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1287 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI