(Circular) (Six-base) MAPSORT of: pFN21AE4533.seq Check: 8389 from: 1 to: 6499 pFN21AE4533 6499 bp With 182 enzymes: SgfI * March 28, 2012 14:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2286 2286 Size: 6499 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3414 4309 278 Size: 53 83 186 936 1878 895 2468 Fragments arranged by size: 2468 1878 936 895 186 83 53 AccI GT'mk_AC Cuts at: 1937 3842 1937 Size: 1905 4594 Acc65I G'GTAC_C Cuts at: 3820 3820 Size: 6499 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2778 4562 5946 6214 1632 Size: 497 649 1784 1384 268 1917 Fragments arranged by size: 1917 1784 1384 649 497 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6302 AflIII A'CryG_T Cuts at: 1203 5398 6375 1203 Size: 4195 977 1327 Fragments arranged by size: 4195 1327 977 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 5228 5228 Size: 6499 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6499 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3714 1184 Size: 2530 3969 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3440 3472 3440 Size: 32 6467 AlwNI CAG_nnn'CTG Cuts at: 2104 4242 5814 2104 Size: 2138 1572 2789 Fragments arranged by size: 2789 2138 1572 ApaI G_GGCC'C Cuts at: 3162 3162 Size: 6499 ApaLI G'TGCA_C Cuts at: 4556 5712 4556 Size: 1156 5343 ApoI r'AATT_y Cuts at: 1493 2250 2887 3808 4034 4186 6295 1493 Size: 757 637 921 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 757 637 226 152 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 2176 2529 2676 3824 1870 Size: 73 233 353 147 1148 4545 Fragments arranged by size: 4545 1148 353 233 147 73 BamHI G'GATC_C Cuts at: 2592 3829 4196 2592 Size: 1237 367 4895 Fragments arranged by size: 4895 1237 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 3320 3820 5275 618 Size: 325 217 627 831 702 500 1455 1842 Fragments arranged by size: 1842 1455 831 702 627 500 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2486 2509 3162 3172 3818 1391 Size: 645 450 23 653 10 646 4072 Fragments arranged by size: 4072 653 646 645 450 23 10 BbeI G_GCGC'C Cuts at: 3324 3324 Size: 6499 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2869 928 Size: 971 970 4558 Fragments arranged by size: 4558 971 970 BbvCI CC'TCA_GC Cuts at: 2499 3104 2499 Size: 605 5894 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4690 4724 1903 Size: 34 2753 34 3678 Fragments arranged by size: 3678 2753 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3206 4391 5601 3206 Size: 1185 1210 4104 Fragments arranged by size: 4104 1210 1185 BclI T'GATC_A Cuts at: 1548 1548 Size: 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5110 136 Size: 107 122 71 631 605 3438 1525 Fragments arranged by size: 3438 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6494 6494 Size: 6499 BlpI GC'TnA_GC Cuts at: 2420 3916 2420 Size: 1496 5003 Bme1580I G_kGCm'C Cuts at: 1790 2558 2990 3162 3346 3552 3693 4560 5716 Size: 768 432 172 184 206 141 867 1156 Cuts at: 5716 1790 Size: 2573 Fragments arranged by size: 2573 1156 867 768 432 206 184 172 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 2602 2665 Size: 416 53 83 399 24 741 444 63 Cuts at: 2665 3448 4230 5188 442 Size: 783 782 958 1753 Fragments arranged by size: 1753 958 783 782 741 444 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 3705 1056 Size: 2649 3850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2461 2500 2191 Size: 270 39 6190 Fragments arranged by size: 6190 270 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2727 3205 5159 1239 Size: 1488 478 1954 2579 Fragments arranged by size: 2579 1954 1488 478 Bpu10I CC'TnA_GC Cuts at: 2030 2237 2499 3104 2030 Size: 207 262 605 5425 Fragments arranged by size: 5425 605 262 207 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4624 5489 5787 4624 Size: 865 298 5336 Fragments arranged by size: 5336 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2162 2798 5162 882 Size: 617 663 636 2364 2219 Fragments arranged by size: 2364 2219 663 636 617 BsaAI yAC'GTr Cuts at: 493 1204 4290 493 Size: 711 3086 2702 Fragments arranged by size: 3086 2702 711 BsaBI GATnn'nnATC Cuts at: 2927 3428 6493 2927 Size: 501 3065 2933 Fragments arranged by size: 3065 2933 501 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3321 3411 4306 4688 Size: 53 83 186 936 1788 90 895 382 Cuts at: 4688 5323 275 Size: 635 1451 Fragments arranged by size: 1788 1451 936 895 635 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 3209 3445 4932 5330 5604 5751 6162 3209 Size: 236 1487 398 274 147 411 3546 Fragments arranged by size: 3546 1487 411 398 274 236 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6274 Fragments arranged by size: 6274 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2262 1186 Size: 1076 5423 BseYI C'CCAG_C Cuts at: 2508 5702 6198 2508 Size: 3194 496 2809 Fragments arranged by size: 3194 2809 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3642 3696 1163 Size: 573 1906 54 3966 Fragments arranged by size: 3966 1906 573 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3949 4710 5738 1540 Size: 275 186 1948 761 1028 2301 Fragments arranged by size: 2301 1948 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 3172 4560 4645 5716 2486 Size: 686 1388 85 1071 3269 Fragments arranged by size: 3269 1388 1071 686 85 BsmI GAATG_Cn' Cuts at: 4010 4103 4010 Size: 93 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2486 2509 2558 2990 3162 3172 Size: 399 246 450 23 49 432 172 10 Cuts at: 3172 3346 3552 3693 3818 4560 4645 5716 1391 Size: 174 206 141 125 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 432 399 246 206 174 172 141 125 85 49 23 10 BspEI T'CCGG_A Cuts at: 3209 6162 3209 Size: 2953 3546 BspHI T'CATG_A Cuts at: 4388 6118 4388 Size: 1730 4769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2286 3854 844 Size: 1442 1568 3489 Fragments arranged by size: 3489 1568 1442 BsrBI CCG'CTC Cuts at: 1394 1823 4386 1394 Size: 429 2563 3507 Fragments arranged by size: 3507 2563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4994 5168 65 Size: 1183 3746 174 1396 Fragments arranged by size: 3746 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5143 5330 1700 Size: 110 3333 187 2869 Fragments arranged by size: 3333 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3736 96 Size: 3640 2859 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6499 BssSI C'ACGA_G Cuts at: 3639 4559 5571 3639 Size: 920 1012 4567 Fragments arranged by size: 4567 1012 920 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6499 BstBI TT'CG_AA Cuts at: 2260 5303 2260 Size: 3043 3456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6499 BstXI CCAn_nnnn'nTGG Cuts at: 2549 2615 2549 Size: 66 6433 BstYI r'GATC_y Cuts at: 1972 2592 3474 3829 4196 4581 4598 6050 6136 Size: 620 882 355 367 385 17 1452 86 Cuts at: 6136 6148 6494 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 882 620 385 367 355 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3771 3771 Size: 6499 BtgI C'CryG_G Cuts at: 513 1065 2409 3163 513 Size: 552 1344 754 3849 Fragments arranged by size: 3849 1344 754 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5760 Fragments arranged by size: 5760 683 56 BtsI GCAGTG_nn' Cuts at: 2288 4015 4809 4829 2288 Size: 1727 794 20 3958 Fragments arranged by size: 3958 1727 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2593 2628 529 Size: 35 2029 35 4400 Fragments arranged by size: 4400 2029 35 35 DraI TTT'AAA Cuts at: 3803 3803 Size: 6499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3565 3946 4835 Size: 54 1425 50 275 18 1735 381 889 Cuts at: 4835 6464 8 Size: 1629 43 Fragments arranged by size: 1735 1629 1425 889 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3946 1537 Size: 275 2134 4090 Fragments arranged by size: 4090 2134 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 2530 2814 3302 3559 4429 1353 Size: 1116 61 284 488 257 870 3423 Fragments arranged by size: 3423 1116 870 488 284 257 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5086 5460 5606 387 Size: 1064 138 3497 374 146 1280 Fragments arranged by size: 3497 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2484 3170 2484 Size: 686 5813 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2727 2778 3205 4562 5159 5946 Size: 393 497 598 51 427 1357 597 787 Cuts at: 5946 6214 1239 Size: 268 1524 Fragments arranged by size: 1524 1357 787 598 597 497 427 393 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 3399 3399 Size: 6499 EcoO109I rG'GnC_Cy Cuts at: 2101 3158 3159 2101 Size: 1057 1 5441 Fragments arranged by size: 5441 1057 1 EcoRI G'AATT_C Cuts at: 3808 4186 3808 Size: 378 6121 EcoRV GAT'ATC Cuts at: 2686 2686 Size: 6499 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 6499 FspI TGC'GCA Cuts at: 5005 5005 Size: 6499 HaeII r_GCGC'y Cuts at: 1678 3324 5646 1678 Size: 1646 2322 2531 Fragments arranged by size: 2531 2322 1646 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3277 3309 3366 3398 5140 Size: 32 135 32 1705 32 57 32 1742 Cuts at: 5140 5172 5214 5246 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1742 1705 135 57 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 2371 3070 3843 4089 669 Size: 988 281 406 27 699 773 246 3079 Fragments arranged by size: 3079 988 773 699 406 281 246 27 HindIII A'AGCT_T Cuts at: 748 2012 748 Size: 1264 5235 HpaI GTT'AAC Cuts at: 3070 4089 3070 Size: 1019 5480 KasI G'GCGC_C Cuts at: 3320 3320 Size: 6499 KpnI G_GTAC'C Cuts at: 3824 3824 Size: 6499 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 6375 6375 Size: 6499 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 2839 5613 5797 1305 Size: 969 565 2774 184 2007 Fragments arranged by size: 2774 2007 969 565 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4677 Fragments arranged by size: 4677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2978 4457 4816 4975 518 Size: 648 1812 1479 359 159 2042 Fragments arranged by size: 2042 1812 1479 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2587 3165 3179 3358 3563 3915 4592 5740 Size: 981 578 14 179 205 352 677 1148 Cuts at: 5740 5985 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 981 677 578 352 245 205 179 14 NarI GG'CG_CC Cuts at: 3321 3321 Size: 6499 NcoI C'CATG_G Cuts at: 513 1065 2409 513 Size: 552 1344 4603 Fragments arranged by size: 4603 1344 552 NdeI CA'TA_TG Cuts at: 387 2380 387 Size: 1993 4506 NheI G'CTAG_C Cuts at: 1052 3701 1052 Size: 2649 3850 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 2766 3857 5402 1132 Size: 97 1537 1091 1545 2229 Fragments arranged by size: 2229 1545 1537 1091 97 PasI CC'CwG_GG Cuts at: 1348 2549 3092 3466 1348 Size: 1201 543 374 4381 Fragments arranged by size: 4381 1201 543 374 PciI A'CATG_T Cuts at: 5398 5398 Size: 6499 PflMI CCAn_nnn'nTGG Cuts at: 1108 3316 1108 Size: 2208 4291 PfoI T'CCnGG_A Cuts at: 2466 2466 Size: 6499 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2101 Size: 6499 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6499 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PspOMI G'GGCC_C Cuts at: 3158 3158 Size: 6499 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 6467 PstI C_TGCA'G Cuts at: 830 2283 2716 3609 3851 830 Size: 1453 433 893 242 3478 Fragments arranged by size: 3478 1453 893 433 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 3358 3563 3358 Size: 205 6294 SacI G_AGCT'C Cuts at: 2486 3172 2486 Size: 686 5813 SacII CC_GC'GG Cuts at: 3166 3166 Size: 6499 SalI G'TCGA_C Cuts at: 1936 3841 1936 Size: 1905 4594 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 4747 6459 4747 Size: 1712 4787 SfcI C'TryA_G Cuts at: 826 1045 2279 2712 3605 3847 4982 5663 5854 Size: 219 1234 433 893 242 1135 681 191 Cuts at: 5854 826 Size: 1471 Fragments arranged by size: 1471 1234 1135 893 681 433 242 219 191 SfoI GGC'GCC Cuts at: 3322 3322 Size: 6499 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 2531 2678 3826 1872 Size: 659 147 1148 4545 Fragments arranged by size: 4545 1148 659 147 SmlI C'TyrA_G Cuts at: 820 1017 1943 4603 5504 5766 820 Size: 197 926 2660 901 262 1553 Fragments arranged by size: 2660 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4290 493 Size: 3797 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 2766 3857 1132 Size: 1634 1091 3774 Fragments arranged by size: 3774 1634 1091 SspI AAT'ATT Cuts at: 5 52 4423 5 Size: 47 4371 2081 Fragments arranged by size: 4371 2081 47 StuI AGG'CCT Cuts at: 1517 2999 3044 1517 Size: 1482 45 4972 Fragments arranged by size: 4972 1482 45 StyI C'CwwG_G Cuts at: 513 1065 2409 3938 513 Size: 552 1344 1529 3074 Fragments arranged by size: 3074 1529 1344 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2621 4526 4543 4696 4881 5326 325 Size: 1255 1041 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 1041 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3736 4745 6457 Size: 274 80 33 51 1443 1759 1009 1712 Cuts at: 6457 96 Size: 138 Fragments arranged by size: 1759 1712 1443 1009 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 6459 6491 3123 Size: 32 3304 32 3131 Fragments arranged by size: 3304 3131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2671 1316 Size: 1355 5144 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3242 3242 Size: 6499 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6499 XmaI C'CCGG_G Cuts at: 1870 2529 2676 3824 1870 Size: 659 147 1148 4545 Fragments arranged by size: 4545 1148 659 147 XmnI GAAnn'nnTTC Cuts at: 2823 3296 4628 2823 Size: 473 1332 4694 Fragments arranged by size: 4694 1332 473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3412 4307 276 Size: 53 83 186 936 1878 895 2468 Fragments arranged by size: 2468 1878 936 895 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SanDI SapI SexAI SfiI SrfI SwaI