(Linear) (Six-base) MAPSORT of: KIEE4533.seq Check: 8325 from: 1 to: 1794 KIEE4533.orf With 182 enzymes: SgfI * March 22, 2012 18:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 281 1794 Size: 281 1513 AatII G_ACGT'C Cuts at: 0 1409 1794 Size: 1409 385 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 773 1794 Size: 124 649 1021 Fragments arranged by size: 1021 649 124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1709 1794 Size: 1709 85 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1435 1467 1794 Size: 1435 32 327 Fragments arranged by size: 1435 327 32 AlwNI CAG_nnn'CTG Cuts at: 0 99 1794 Size: 99 1695 ApaI G_GGCC'C Cuts at: 0 1157 1794 Size: 1157 637 ApoI r'AATT_y Cuts at: 0 245 882 1794 Size: 245 637 912 Fragments arranged by size: 912 637 245 AvaI C'yCGr_G Cuts at: 0 171 524 671 1794 Size: 171 353 147 1123 Fragments arranged by size: 1123 353 171 147 BamHI G'GATC_C Cuts at: 0 587 1794 Size: 587 1207 BanI G'GyrC_C Cuts at: 0 613 1315 1794 Size: 613 702 479 Fragments arranged by size: 702 613 479 BanII G_rGCy'C Cuts at: 0 31 481 504 1157 1167 1794 Size: 31 450 23 653 10 627 Fragments arranged by size: 653 627 450 31 23 10 BbeI G_GCGC'C Cuts at: 0 1319 1794 Size: 1319 475 BbsI GAAGACnn'nnnn_ Cuts at: 0 864 1794 Size: 864 930 BbvCI CC'TCA_GC Cuts at: 0 494 1099 1794 Size: 494 605 695 Fragments arranged by size: 695 605 494 BciVI GTATCCnnnnn_n' Cuts at: 0 1201 1794 Size: 1201 593 BlpI GC'TnA_GC Cuts at: 0 415 1794 Size: 415 1379 Bme1580I G_kGCm'C Cuts at: 0 553 985 1157 1341 1547 1688 1794 Size: 553 432 172 184 206 141 106 Fragments arranged by size: 553 432 206 184 172 141 106 BmrI ACTGGGnnnn_n' Cuts at: 0 153 597 660 1443 1794 Size: 153 444 63 783 351 Fragments arranged by size: 783 444 351 153 63 BmtI G_CTAG'C Cuts at: 0 1700 1794 Size: 1700 94 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 186 456 495 1794 Size: 186 270 39 1299 Fragments arranged by size: 1299 270 186 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 1200 1794 Size: 722 478 594 Fragments arranged by size: 722 594 478 Bpu10I CC'TnA_GC Cuts at: 0 25 232 494 1099 1794 Size: 25 207 262 605 695 Fragments arranged by size: 695 605 262 207 25 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 793 1794 Size: 157 636 1001 Fragments arranged by size: 1001 636 157 BsaBI GATnn'nnATC Cuts at: 0 922 1423 1794 Size: 922 501 371 Fragments arranged by size: 922 501 371 BsaHI Gr'CG_yC Cuts at: 0 1316 1406 1794 Size: 1316 90 388 Fragments arranged by size: 1316 388 90 BsaWI w'CCGG_w Cuts at: 0 1204 1440 1794 Size: 1204 236 354 Fragments arranged by size: 1204 354 236 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 257 1794 Size: 257 1537 BseYI C'CCAG_C Cuts at: 0 503 1794 Size: 503 1291 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1637 1691 1794 Size: 1637 54 103 Fragments arranged by size: 1637 103 54 BsiHKAI G_wGCw'C Cuts at: 0 481 1167 1794 Size: 481 686 627 Fragments arranged by size: 686 627 481 Bsp1286I G_dGCh'C Cuts at: 0 31 481 504 553 985 1157 1167 1341 Size: 31 450 23 49 432 172 10 174 Cuts at: 1341 1547 1688 1794 Size: 206 141 106 Fragments arranged by size: 450 432 206 174 172 141 106 49 31 23 10 BspEI T'CCGG_A Cuts at: 0 1204 1794 Size: 1204 590 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 281 1794 Size: 281 1513 BsrGI T'GTAC_A Cuts at: 0 1731 1794 Size: 1731 63 BssSI C'ACGA_G Cuts at: 0 1634 1794 Size: 1634 160 BstBI TT'CG_AA Cuts at: 0 255 1794 Size: 255 1539 BstXI CCAn_nnnn'nTGG Cuts at: 0 544 610 1794 Size: 544 66 1184 Fragments arranged by size: 1184 544 66 BstYI r'GATC_y Cuts at: 0 587 1469 1794 Size: 587 882 325 Fragments arranged by size: 882 587 325 Bsu36I CC'TnA_GG Cuts at: 0 1766 1794 Size: 1766 28 BtgI C'CryG_G Cuts at: 0 404 1158 1794 Size: 404 754 636 Fragments arranged by size: 754 636 404 BtsI GCAGTG_nn' Cuts at: 0 283 1794 Size: 283 1511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 588 623 1794 Size: 588 35 1171 Fragments arranged by size: 1171 588 35 EaeI y'GGCC_r Cuts at: 0 1560 1794 Size: 1560 234 EarI CTCTTCn'nnn_ Cuts at: 0 464 525 809 1297 1554 1794 Size: 464 61 284 488 257 240 Fragments arranged by size: 488 464 284 257 240 61 EcoICRI GAG'CTC Cuts at: 0 479 1165 1794 Size: 479 686 629 Fragments arranged by size: 686 629 479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 722 773 1200 1794 Size: 124 598 51 427 594 Fragments arranged by size: 598 594 427 124 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 1394 1794 Size: 1394 400 EcoO109I rG'GnC_Cy Cuts at: 0 96 1153 1154 1794 Size: 96 1057 1 640 Fragments arranged by size: 1057 640 96 1 EcoRV GAT'ATC Cuts at: 0 681 1794 Size: 681 1113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 651 1794 Size: 651 1143 HaeII r_GCGC'y Cuts at: 0 1319 1794 Size: 1319 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1272 1304 1361 1393 1794 Size: 1272 32 57 32 401 Fragments arranged by size: 1272 401 57 32 32 HincII GTy'rAC Cuts at: 0 339 366 1065 1794 Size: 339 27 699 729 Fragments arranged by size: 729 699 339 27 HindIII A'AGCT_T Cuts at: 0 7 1794 Size: 7 1787 HpaI GTT'AAC Cuts at: 0 1065 1794 Size: 1065 729 KasI G'GCGC_C Cuts at: 0 1315 1794 Size: 1315 479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 269 834 1794 Size: 269 565 960 Fragments arranged by size: 960 565 269 MslI CAynn'nnrTG Cuts at: 0 973 1794 Size: 973 821 MspA1I CmG'CkG Cuts at: 0 582 1160 1174 1353 1558 1794 Size: 582 578 14 179 205 236 Fragments arranged by size: 582 578 236 205 179 14 NarI GG'CG_CC Cuts at: 0 1316 1794 Size: 1316 478 NcoI C'CATG_G Cuts at: 0 404 1794 Size: 404 1390 NdeI CA'TA_TG Cuts at: 0 375 1794 Size: 375 1419 NheI G'CTAG_C Cuts at: 0 1696 1794 Size: 1696 98 NspI r_CATG'y Cuts at: 0 761 1794 Size: 761 1033 PasI CC'CwG_GG Cuts at: 0 544 1087 1461 1794 Size: 544 543 374 333 Fragments arranged by size: 544 543 374 333 PflMI CCAn_nnn'nTGG Cuts at: 0 1311 1794 Size: 1311 483 PfoI T'CCnGG_A Cuts at: 0 461 1794 Size: 461 1333 PpuMI rG'GwC_Cy Cuts at: 0 96 1794 Size: 96 1698 PspOMI G'GGCC_C Cuts at: 0 1153 1794 Size: 1153 641 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 27 1794 Size: 27 1767 PstI C_TGCA'G Cuts at: 0 278 711 1604 1794 Size: 278 433 893 190 Fragments arranged by size: 893 433 278 190 PvuII CAG'CTG Cuts at: 0 1353 1558 1794 Size: 1353 205 236 Fragments arranged by size: 1353 236 205 SacI G_AGCT'C Cuts at: 0 481 1167 1794 Size: 481 686 627 Fragments arranged by size: 686 627 481 SacII CC_GC'GG Cuts at: 0 1161 1794 Size: 1161 633 SfcI C'TryA_G Cuts at: 0 274 707 1600 1794 Size: 274 433 893 194 Fragments arranged by size: 893 433 274 194 SfoI GGC'GCC Cuts at: 0 1317 1794 Size: 1317 477 SmaI CCC'GGG Cuts at: 0 526 673 1794 Size: 526 147 1121 Fragments arranged by size: 1121 526 147 SphI G_CATG'C Cuts at: 0 761 1794 Size: 761 1033 StuI AGG'CCT Cuts at: 0 994 1039 1794 Size: 994 45 755 Fragments arranged by size: 994 755 45 StyI C'CwwG_G Cuts at: 0 404 1794 Size: 404 1390 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 616 1794 Size: 616 1178 TatI w'GTAC_w Cuts at: 0 1731 1794 Size: 1731 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1118 1150 1794 Size: 1118 32 644 Fragments arranged by size: 1118 644 32 Tth111I GACn'n_nGTC Cuts at: 0 666 1794 Size: 666 1128 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1237 1794 Size: 1237 557 XmaI C'CCGG_G Cuts at: 0 524 671 1794 Size: 524 147 1123 Fragments arranged by size: 1123 524 147 XmnI GAAnn'nnTTC Cuts at: 0 818 1291 1794 Size: 818 473 503 Fragments arranged by size: 818 503 473 ZraI GAC'GTC Cuts at: 0 1407 1794 Size: 1407 387 Enzymes that do cut: AarI AatII AcuI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrGI BssSI BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI MspA1I NarI NcoI NdeI NheI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII SfcI SfoI SmaI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BglI BglII BmgBI BpuEI BsaAI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRI FseI FspI FspAI KpnI MfeI MluI MscI NaeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI