Restriction map for
pFN21AB6187
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstYI BstZ17I Bsu36I DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HindIII HpaI
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1457 GGTACC G^GTACC
AccII 1738 CGCG CG^CG
AcsI 910 AAATTC [GA]^AATT[CT]
AflIII 109 ACATGT A^C[GA][CT]GT
AlwNI 122 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 910 AAATTC [GA]^AATT[CT]
Asp718I 1457 GGTACC G^GTACC
AvaI 647 CCCGAG C^[CT]CG[GA]G
BanII 579 GGGCTC G[GA]GC[CT]^C
BciVI 1011 GGATAC GTATCC(6/5)
BglI 1057 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1052 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1052 GCTCAGC GC^T[ACGT]AGC
BsaWI 1008 ACCGGA [AT]^CCGG[AT]
BseRI 1770 CTCCTC GAGGAG(10/8)
BsgI 1931 CTGCAC GTGCAG(16/14)
BsmI 393 GAATGC GAATGC(1/-1)
BsoBI 647 CCCGAG C^[CT]CG[GA]G
BspDI 1861 ATCGAT AT^CGAT
BspLU11I 109 ACATGT A^CATGT
BsrBI 1500 CCGCTC CCGCTC(-3/-3)
BsrDI 133 CATTGC GCAATG(2/0)
BssSI 1855 CACGAG CACGAG(-5/-1)
BstAPI 1631 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 65 GGTCACC G^GT[ACGT]ACC
BstPI 65 GGTCACC G^GT[ACGT]ACC
BstUI 1738 CGCG CG^CG
BstXI 330 CCAGCCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1052 GCTCAGC GC^T[ACGT]AGC
ClaI 1861 ATCGAT AT^CGAT
CpoI 1718 CGGACCG CG^G[AT]CCG
DrdI 1410 GACTCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1254 CCATGG C^C[GA][CT]GG
EaeI 1758 TGGCCG [CT]^GGCC[GA]
EarI 921 GAAGAG CTCTTC(1/4)
EcoNI 1565 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 65 GGTCACC G^GT[ACGT]ACC
HaeII 123 AGCGCC [GA]GCGC^[CT]
KpnI 1457 GGTACC GGTAC^C
Ksp632I 921 GAAGAG CTCTTC(1/4)
MaeII 1334 ACGT A^CGT
MseI 1654 TTAA T^TAA
MvnI 1738 CGCG CG^CG
NcoI 1254 CCATGG C^CATGG
NspI 109 ACATGT [GA]CATG^[CT]
PflMI 373 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 694 CTGCAG CTGCA^G
RsrII 1718 CGGACCG CG^G[AT]CCG
SexAI 1464 ACCAGGT A^CC[AT]GGT
StuI 1196 AGGCCT AGG^CCT
Tru9I 1654 TTAA T^TAA
Van91I 373 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 785 CCAAAACTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 1816 GATCC GGATC(4/5)
1660 GGATC
AviII 1628 TGCGCA TGC^GCA
1886 TGCGCA
BbvCI 1619 CCTCAGC CCTCAGC(-5/-2)
1819 CCTCAGC
BfaI 73 CTAG C^TAG
517 CTAG
BmyI 254 GTGCCC G[AGT]GC[ACT]^C
579 GGGCTC
BsmAI 650 GAGAC GTCTC(1/5)
686 GAGAC
Bsp1286I 254 GTGCCC G[AGT]GC[ACT]^C
579 GGGCTC
BspMI 692 ACCTGC ACCTGC(4/8)
978 ACCTGC
BsrFI 1577 ACCGGC [GA]^CCGG[CT]
1958 ACCGGC
BsrI 629 ACTGG ACTGG(1/-1)
682 ACTGG
Cfr10I 1577 ACCGGC [GA]^CCGG[CT]
1958 ACCGGC
EcoT14I 1254 CCATGG C^C[AT][AT]GG
1505 CCTTGG
FspI 1628 TGCGCA TGC^GCA
1886 TGCGCA
HgaI 426 GACGC GACGC(5/10)
397 GCGTC
HincII 313 GTTGAC GT[CT]^[GA]AC
1318 GTCAAC
HindII 313 GTTGAC GT[CT]^[GA]AC
1318 GTCAAC
MaeI 73 CTAG C^TAG
517 CTAG
MslI 1255 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1896 CATGATGATG
SmlI 1243 CTCAAG C^T[CT][GA]AG
1936 CTCAAG
StyI 1254 CCATGG C^C[AT][AT]GG
1505 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbsI BbvI BcnI BpmI BpuAI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I