(Circular) (Six-base) MAPSORT of: pFN21AB6187.seq Check: 2882 from: 1 to: 6685 pFN21AB6187 6685 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2992 2992 Size: 6685 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4028 1937 Size: 2091 4594 Acc65I G'GTAC_C Cuts at: 3462 4006 3462 Size: 544 6141 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3223 3944 3989 4748 6132 6400 1632 Size: 1591 721 45 759 1384 268 1917 Fragments arranged by size: 1917 1591 1384 759 721 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 2114 5584 6561 1203 Size: 911 3470 977 1327 Fragments arranged by size: 3470 1327 977 911 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3896 1184 Size: 2712 3973 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 2207 Size: 32 6653 AlwNI CAG_nnn'CTG Cuts at: 2132 4428 6000 2132 Size: 2296 1572 2817 Fragments arranged by size: 2817 2296 1572 ApaLI G'TGCA_C Cuts at: 4742 5898 4742 Size: 1156 5529 ApoI r'AATT_y Cuts at: 1493 2915 3994 4220 4372 6481 1493 Size: 1422 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1422 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 5239 160 Size: 872 4207 1606 Fragments arranged by size: 4207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 2652 4010 1870 Size: 73 709 1358 4545 Fragments arranged by size: 4545 1358 709 73 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 2637 2711 3462 3579 Size: 325 217 627 471 379 74 751 117 Cuts at: 3579 4006 5461 618 Size: 427 1455 1842 Fragments arranged by size: 1842 1455 751 627 471 427 379 325 217 117 74 BanII G_rGCy'C Cuts at: 1391 2588 4004 1391 Size: 1197 1416 4072 Fragments arranged by size: 4072 1416 1197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 2435 3229 928 Size: 971 498 38 794 4384 Fragments arranged by size: 4384 971 794 498 38 BbvCI CC'TCA_GC Cuts at: 3625 3825 3625 Size: 200 6485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4876 4910 1903 Size: 34 50 34 2855 34 3678 Fragments arranged by size: 3678 2855 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3010 4577 5787 3010 Size: 1567 1210 3908 Fragments arranged by size: 3908 1567 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6685 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3068 5296 136 Size: 107 122 71 631 605 1396 2228 1525 Fragments arranged by size: 2228 1525 1396 631 605 122 107 71 BglII A'GATC_T Cuts at: 6680 6680 Size: 6685 BlpI GC'TnA_GC Cuts at: 3058 4102 3058 Size: 1044 5641 Bme1580I G_kGCm'C Cuts at: 1790 2263 4746 5902 1790 Size: 473 2483 1156 2573 Fragments arranged by size: 2573 2483 1156 473 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4416 5374 442 Size: 416 53 83 399 24 2999 958 1753 Fragments arranged by size: 2999 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2976 3292 2976 Size: 316 6369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2709 3374 3631 3755 5345 1239 Size: 1470 665 257 124 1590 2579 Fragments arranged by size: 2579 1590 1470 665 257 124 Bpu10I CC'TnA_GC Cuts at: 3625 3825 3625 Size: 200 6485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3233 3926 4810 5675 5973 3233 Size: 693 884 865 298 3945 Fragments arranged by size: 3945 884 865 693 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5348 882 Size: 617 3849 2219 Fragments arranged by size: 3849 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4476 493 Size: 711 3272 2702 Fragments arranged by size: 3272 2702 711 BsaBI GATnn'nnATC Cuts at: 6679 6679 Size: 6685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4492 4874 5509 275 Size: 53 83 186 936 2959 382 635 1451 Fragments arranged by size: 2959 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3013 5118 5516 5790 5937 6348 3013 Size: 2105 398 274 147 411 3350 Fragments arranged by size: 3350 2105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2989 3019 1178 Size: 30 165 30 1586 30 4844 Fragments arranged by size: 4844 1586 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3766 1186 Size: 2580 4105 BseYI C'CCAG_C Cuts at: 2125 2560 2771 3053 3316 3928 5888 6384 2125 Size: 435 211 282 263 612 1960 496 2426 Fragments arranged by size: 2426 1960 612 496 435 282 263 211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3921 1163 Size: 573 2185 3927 Fragments arranged by size: 3927 2185 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4746 4831 5902 4746 Size: 85 1071 5529 Fragments arranged by size: 5529 1071 85 BsmI GAATG_Cn' Cuts at: 2404 4196 4289 2404 Size: 1792 93 4800 Fragments arranged by size: 4800 1792 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2588 4004 4746 4831 5902 1391 Size: 399 473 325 1416 742 85 1071 2174 Fragments arranged by size: 2174 1416 1071 742 473 399 325 85 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 4574 6304 4574 Size: 1730 4955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 2992 4040 844 Size: 1862 286 1048 3489 Fragments arranged by size: 3489 1862 1048 286 BsrBI CCG'CTC Cuts at: 1394 1823 3507 4572 1394 Size: 429 1684 1065 3507 Fragments arranged by size: 3507 1684 1065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 5180 5354 65 Size: 1183 889 3043 174 1396 Fragments arranged by size: 3043 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3582 3963 5329 5516 1700 Size: 110 1772 381 1366 187 2869 Fragments arranged by size: 2869 1772 1366 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6685 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 3860 4745 5757 3860 Size: 885 1012 4788 Fragments arranged by size: 4788 1012 885 BstAPI GCAn_nnn'nTGC Cuts at: 794 3642 794 Size: 2848 3837 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 2070 1184 Size: 886 5799 BstXI CCAn_nnnn'nTGG Cuts at: 2342 2342 Size: 6685 BstYI r'GATC_y Cuts at: 1972 4015 4382 4767 4784 6236 6322 6334 6680 Size: 2043 367 385 17 1452 86 12 346 Cuts at: 6680 1972 Size: 1977 Fragments arranged by size: 2043 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3259 513 Size: 552 2194 3939 Fragments arranged by size: 3939 2194 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2469 3759 487 Size: 683 56 1243 1290 3413 Fragments arranged by size: 3413 1290 1243 683 56 BtsI GCAGTG_nn' Cuts at: 3429 3618 3901 4201 4995 5015 3429 Size: 189 283 300 794 20 5099 Fragments arranged by size: 5099 794 300 283 189 20 ClaI AT'CG_AT Cuts at: 1554 3867 1554 Size: 2313 4372 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2215 2227 2250 2262 3541 3576 529 Size: 35 1651 12 23 12 1279 35 3638 Fragments arranged by size: 3638 1651 1279 35 35 23 12 12 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3421 5692 809 Size: 507 2105 2271 1802 Fragments arranged by size: 2271 2105 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3763 4132 5021 Size: 54 1425 50 275 18 1933 369 889 Cuts at: 5021 6650 8 Size: 1629 43 Fragments arranged by size: 1933 1629 1425 889 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2921 4615 1353 Size: 1568 1694 3423 Fragments arranged by size: 3423 1694 1568 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5272 5646 5792 387 Size: 1064 138 3683 374 146 1280 Fragments arranged by size: 3683 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 3223 3374 3631 3755 3944 3989 Size: 393 1077 514 151 257 124 189 45 Cuts at: 3989 4748 5345 6132 6400 1239 Size: 759 597 787 268 1524 Fragments arranged by size: 1524 1077 787 759 597 514 393 268 257 189 151 124 45 EcoNI CCTnn'n_nnAGG Cuts at: 3574 3574 Size: 6685 EcoO109I rG'GnC_Cy Cuts at: 2047 2206 2622 2933 3070 3113 3769 3877 3932 Size: 159 416 311 137 43 656 108 55 Cuts at: 3932 2047 Size: 4800 Fragments arranged by size: 4800 656 416 311 159 137 108 55 43 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 FspI TGC'GCA Cuts at: 3635 3893 5191 3635 Size: 258 1298 5129 Fragments arranged by size: 5129 1298 258 HaeII r_GCGC'y Cuts at: 1678 2132 5832 1678 Size: 454 3700 2531 Fragments arranged by size: 3700 2531 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 3527 3559 3674 Size: 32 135 32 896 32 1027 32 115 Cuts at: 3674 3706 3761 3793 5326 5358 5400 5432 1373 Size: 32 55 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 1027 896 135 115 55 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3325 4029 4275 669 Size: 988 281 382 1005 704 246 3079 Fragments arranged by size: 3079 1005 988 704 382 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 3466 4010 3466 Size: 544 6141 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2227 3702 5799 5983 1305 Size: 922 1475 2097 184 2007 Fragments arranged by size: 2097 2007 1475 922 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4863 Fragments arranged by size: 4863 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3264 3905 4643 5002 5161 518 Size: 648 2098 641 738 359 159 2042 Fragments arranged by size: 2098 2042 738 648 641 359 159 MspA1I CmG'CkG Cuts at: 1606 2327 2903 3629 4101 4778 5926 6171 1606 Size: 721 576 726 472 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 721 677 576 472 245 NcoI C'CATG_G Cuts at: 513 1065 3259 513 Size: 552 2194 3939 Fragments arranged by size: 3939 2194 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 2118 4043 5588 1132 Size: 97 889 1925 1545 2229 Fragments arranged by size: 2229 1925 1545 889 97 PasI CC'CwG_GG Cuts at: 1348 3716 1348 Size: 2368 4317 PciI A'CATG_T Cuts at: 2114 5584 2114 Size: 3470 3215 PflMI CCAn_nnn'nTGG Cuts at: 1108 2384 1108 Size: 1276 5409 PfoI T'CCnGG_A Cuts at: 3351 3810 3351 Size: 459 6226 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2206 2933 3769 2047 Size: 159 727 836 4963 Fragments arranged by size: 4963 836 727 159 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PstI C_TGCA'G Cuts at: 830 2703 4037 830 Size: 1873 1334 3478 Fragments arranged by size: 3478 1873 1334 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 PvuII CAG'CTG Cuts at: 2327 2903 3629 2327 Size: 576 726 5383 Fragments arranged by size: 5383 726 576 RsrII CG'GwC_CG Cuts at: 3724 3724 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SanDI GG'GwC_CC Cuts at: 3769 3769 Size: 6685 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SexAI A'CCwGG_T Cuts at: 3469 3469 Size: 6685 SfcI C'TryA_G Cuts at: 826 1045 2017 2372 2699 4033 5168 5849 6040 Size: 219 972 355 327 1334 1135 681 191 Cuts at: 6040 826 Size: 1471 Fragments arranged by size: 1471 1334 1135 972 681 355 327 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3248 3941 4789 5690 5952 820 Size: 197 926 1305 693 848 901 262 1553 Fragments arranged by size: 1553 1305 926 901 848 693 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 4043 1132 Size: 2911 3774 SspI AAT'ATT Cuts at: 5 52 4609 5 Size: 47 4557 2081 Fragments arranged by size: 4557 2081 47 StuI AGG'CCT Cuts at: 1517 3203 1517 Size: 1686 4999 StyI C'CwwG_G Cuts at: 513 1065 3259 3510 4124 513 Size: 552 2194 251 614 3074 Fragments arranged by size: 3074 2194 614 552 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2601 2872 3741 4712 4729 4882 5067 Size: 1255 1021 271 869 971 17 153 185 Cuts at: 5067 5512 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1021 971 869 445 271 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3441 3912 4931 Size: 274 80 33 51 1443 1464 471 1019 Cuts at: 4931 6643 96 Size: 1712 138 Fragments arranged by size: 1712 1464 1443 1019 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6645 6677 6645 Size: 32 6653 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 2797 Size: 6685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 4814 4814 Size: 6685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII SapI SfiI SfoI SrfI SwaI