Restriction map for
pFN21AB6187
(Linear) (Six-base) MAPSORT of: KIBB6187.seq Check: 4336 from: 1 to: 1980
KIBB6187.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 987 1980
Size: 987 993
Acc65I G'GTAC_C
Cuts at: 0 1457 1980
Size: 1457 523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1218 1939 1980
Size: 1218 721 41
Fragments arranged by size:
1218 721 41
AflIII A'CryG_T
Cuts at: 0 109 1980
Size: 109 1871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1891 1980
Size: 1891 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 1980
Size: 202 32 1746
Fragments arranged by size:
1746 202 32
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1980
Size: 127 1853
ApoI r'AATT_y
Cuts at: 0 910 1980
Size: 910 1070
AvaI C'yCGr_G
Cuts at: 0 647 1980
Size: 647 1333
BanI G'GyrC_C
Cuts at: 0 253 632 706 1457 1574 1980
Size: 253 379 74 751 117 406
Fragments arranged by size:
751 406 379 253 117 74
BanII G_rGCy'C
Cuts at: 0 583 1980
Size: 583 1397
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 430 1224 1980
Size: 392 38 794 756
Fragments arranged by size:
794 756 392 38
BbvCI CC'TCA_GC
Cuts at: 0 1620 1820 1980
Size: 1620 200 160
Fragments arranged by size:
1620 200 160
BciVI GTATCCnnnnn_n'
Cuts at: 0 1005 1980
Size: 1005 975
BglI GCCn_nnn'nGGC
Cuts at: 0 1063 1980
Size: 1063 917
BlpI GC'TnA_GC
Cuts at: 0 1053 1980
Size: 1053 927
Bme1580I G_kGCm'C
Cuts at: 0 258 1980
Size: 258 1722
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 971 1287 1980
Size: 971 316 693
Fragments arranged by size:
971 693 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1369 1626 1750 1980
Size: 704 665 257 124 230
Fragments arranged by size:
704 665 257 230 124
Bpu10I CC'TnA_GC
Cuts at: 0 1620 1820 1980
Size: 1620 200 160
Fragments arranged by size:
1620 200 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1921 1980
Size: 1228 693 59
Fragments arranged by size:
1228 693 59
BsaWI w'CCGG_w
Cuts at: 0 1008 1980
Size: 1008 972
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 984 1014 1980
Size: 984 30 966
Fragments arranged by size:
984 966 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1761 1980
Size: 1761 219
BseYI C'CCAG_C
Cuts at: 0 120 555 766 1048 1311 1923 1980
Size: 120 435 211 282 263 612 57
Fragments arranged by size:
612 435 282 263 211 120 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1916 1980
Size: 1916 64
BsmI GAATG_Cn'
Cuts at: 0 399 1980
Size: 399 1581
Bsp1286I G_dGCh'C
Cuts at: 0 258 583 1980
Size: 258 325 1397
Fragments arranged by size:
1397 325 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 987 1980
Size: 701 286 993
Fragments arranged by size:
993 701 286
BsrBI CCG'CTC
Cuts at: 0 1502 1980
Size: 1502 478
BsrDI GCAATG_nn'
Cuts at: 0 132 1980
Size: 132 1848
BsrFI r'CCGG_y
Cuts at: 0 1577 1958 1980
Size: 1577 381 22
Fragments arranged by size:
1577 381 22
BssSI C'ACGA_G
Cuts at: 0 1855 1980
Size: 1855 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1637 1980
Size: 1637 343
BstEII G'GTnAC_C
Cuts at: 0 65 1980
Size: 65 1915
BstXI CCAn_nnnn'nTGG
Cuts at: 0 337 1980
Size: 337 1643
BtgI C'CryG_G
Cuts at: 0 1254 1980
Size: 1254 726
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 464 1754 1980
Size: 464 1290 226
Fragments arranged by size:
1290 464 226
BtsI GCAGTG_nn'
Cuts at: 0 1424 1613 1896 1980
Size: 1424 189 283 84
Fragments arranged by size:
1424 283 189 84
ClaI AT'CG_AT
Cuts at: 0 1862 1980
Size: 1862 118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 210 222 245 257 1536 1571 1980
Size: 210 12 23 12 1279 35 409
Fragments arranged by size:
1279 409 210 35 23 12 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1416 1980
Size: 1416 564
EaeI y'GGCC_r
Cuts at: 0 1758 1980
Size: 1758 222
EarI CTCTTCn'nnn_
Cuts at: 0 916 1980
Size: 916 1064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1218 1369 1626 1750 1939 1980
Size: 704 514 151 257 124 189 41
Fragments arranged by size:
704 514 257 189 151 124 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1569 1980
Size: 1569 411
EcoO109I rG'GnC_Cy
Cuts at: 0 42 201 617 928 1065 1108 1764 1872
Size: 42 159 416 311 137 43 656 108
Cuts at: 1872 1927 1980
Size: 55 53
Fragments arranged by size:
656 416 311 159 137 108 55 53
43 42
FspI TGC'GCA
Cuts at: 0 1630 1888 1980
Size: 1630 258 92
Fragments arranged by size:
1630 258 92
HaeII r_GCGC'y
Cuts at: 0 127 1980
Size: 127 1853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1522 1554 1669 1701 1756 1788
Size: 463 32 1027 32 115 32 55 32
Cuts at: 1788 1980
Size: 192
Fragments arranged by size:
1027 463 192 115 55 32 32 32
32
HincII GTy'rAC
Cuts at: 0 315 1320 1980
Size: 315 1005 660
Fragments arranged by size:
1005 660 315
KpnI G_GTAC'C
Cuts at: 0 1461 1980
Size: 1461 519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 1697 1980
Size: 222 1475 283
Fragments arranged by size:
1475 283 222
MslI CAynn'nnrTG
Cuts at: 0 1259 1900 1980
Size: 1259 641 80
Fragments arranged by size:
1259 641 80
MspA1I CmG'CkG
Cuts at: 0 322 898 1624 1980
Size: 322 576 726 356
Fragments arranged by size:
726 576 356 322
NcoI C'CATG_G
Cuts at: 0 1254 1980
Size: 1254 726
NspI r_CATG'y
Cuts at: 0 113 1980
Size: 113 1867
PasI CC'CwG_GG
Cuts at: 0 1711 1980
Size: 1711 269
PciI A'CATG_T
Cuts at: 0 109 1980
Size: 109 1871
PflMI CCAn_nnn'nTGG
Cuts at: 0 379 1980
Size: 379 1601
PfoI T'CCnGG_A
Cuts at: 0 1346 1805 1980
Size: 1346 459 175
Fragments arranged by size:
1346 459 175
PpuMI rG'GwC_Cy
Cuts at: 0 42 201 928 1764 1980
Size: 42 159 727 836 216
Fragments arranged by size:
836 727 216 159 42
PstI C_TGCA'G
Cuts at: 0 698 1980
Size: 698 1282
PvuII CAG'CTG
Cuts at: 0 322 898 1624 1980
Size: 322 576 726 356
Fragments arranged by size:
726 576 356 322
RsrII CG'GwC_CG
Cuts at: 0 1719 1980
Size: 1719 261
SanDI GG'GwC_CC
Cuts at: 0 1764 1980
Size: 1764 216
SexAI A'CCwGG_T
Cuts at: 0 1464 1980
Size: 1464 516
SfcI C'TryA_G
Cuts at: 0 12 367 694 1980
Size: 12 355 327 1286
Fragments arranged by size:
1286 355 327 12
SmlI C'TyrA_G
Cuts at: 0 1243 1936 1980
Size: 1243 693 44
Fragments arranged by size:
1243 693 44
StuI AGG'CCT
Cuts at: 0 1198 1980
Size: 1198 782
StyI C'CwwG_G
Cuts at: 0 1254 1505 1980
Size: 1254 251 475
Fragments arranged by size:
1254 475 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 596 867 1736 1980
Size: 596 271 869 244
Fragments arranged by size:
869 596 271 244
TatI w'GTAC_w
Cuts at: 0 1436 1907 1980
Size: 1436 471 73
Fragments arranged by size:
1436 471 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1980
Size: 792 1188
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlfI AloI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BglI BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI ClaI
CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KpnI MmeI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PpuMI PstI PvuII
RsrII SanDI SexAI SfcI SmlI StuI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BclI BfrBI BglII BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SacI SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI