(Linear) (Six-base) MAPSORT of: KIEE6708.seq Check: 7662 from: 1 to: 2217 KIEE6708.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1223 1365 2217 Size: 1223 142 852 Fragments arranged by size: 1223 852 142 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 769 1213 1735 2217 Size: 502 267 444 522 482 Fragments arranged by size: 522 502 482 444 267 AfeI AGC'GCT Cuts at: 0 1462 2217 Size: 1462 755 AflIII A'CryG_T Cuts at: 0 701 1494 2217 Size: 701 793 723 Fragments arranged by size: 793 723 701 AlwNI CAG_nnn'CTG Cuts at: 0 1203 2217 Size: 1203 1014 ApaI G_GGCC'C Cuts at: 0 1158 2217 Size: 1158 1059 AvaI C'yCGr_G Cuts at: 0 229 1253 2217 Size: 229 1024 964 Fragments arranged by size: 1024 964 229 BamHI G'GATC_C Cuts at: 0 93 2217 Size: 93 2124 BanI G'GyrC_C Cuts at: 0 36 627 651 1015 2217 Size: 36 591 24 364 1202 Fragments arranged by size: 1202 591 364 36 24 BanII G_rGCy'C Cuts at: 0 222 1158 1490 1958 2217 Size: 222 936 332 468 259 Fragments arranged by size: 936 468 332 259 222 BbeI G_GCGC'C Cuts at: 0 40 2217 Size: 40 2177 BbsI GAAGACnn'nnnn_ Cuts at: 0 1178 2217 Size: 1178 1039 BbvCI CC'TCA_GC Cuts at: 0 1088 1642 2217 Size: 1088 554 575 Fragments arranged by size: 1088 575 554 BlpI GC'TnA_GC Cuts at: 0 1952 2217 Size: 1952 265 Bme1580I G_kGCm'C Cuts at: 0 1143 1158 2217 Size: 1143 15 1059 Fragments arranged by size: 1143 1059 15 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 421 775 793 1018 1268 1417 1615 Size: 177 244 354 18 225 250 149 198 Cuts at: 1615 1726 1897 2217 Size: 111 171 320 Fragments arranged by size: 354 320 250 244 225 198 177 171 149 111 18 Bpu10I CC'TnA_GC Cuts at: 0 1088 1642 2217 Size: 1088 554 575 Fragments arranged by size: 1088 575 554 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 718 1282 2217 Size: 355 363 564 935 Fragments arranged by size: 935 564 363 355 BsaI GGTCTCn'nnnn_ Cuts at: 0 1561 2217 Size: 1561 656 BsaAI yAC'GTr Cuts at: 0 1794 2217 Size: 1794 423 BsaHI Gr'CG_yC Cuts at: 0 4 37 1163 2217 Size: 4 33 1126 1054 Fragments arranged by size: 1126 1054 33 4 BsaWI w'CCGG_w Cuts at: 0 599 2217 Size: 599 1618 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 991 1021 1266 1296 2217 Size: 175 30 786 30 245 30 921 Fragments arranged by size: 921 786 245 175 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 754 820 1429 1714 2164 2176 2217 Size: 33 721 66 609 285 450 12 41 Fragments arranged by size: 721 609 450 285 66 41 33 12 BseYI C'CCAG_C Cuts at: 0 898 2217 Size: 898 1319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 1195 1747 1855 2217 Size: 147 1048 552 108 362 Fragments arranged by size: 1048 552 362 147 108 BsiEI CG_ry'CG Cuts at: 0 33 970 2217 Size: 33 937 1247 Fragments arranged by size: 1247 937 33 BsiHKAI G_wGCw'C Cuts at: 0 1439 1958 2217 Size: 1439 519 259 Fragments arranged by size: 1439 519 259 Bsp1286I G_dGCh'C Cuts at: 0 222 1143 1158 1439 1490 1958 2217 Size: 222 921 15 281 51 468 259 Fragments arranged by size: 921 468 281 259 222 51 15 BspEI T'CCGG_A Cuts at: 0 599 2217 Size: 599 1618 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1217 1223 1365 2217 Size: 1217 6 142 852 Fragments arranged by size: 1217 852 142 6 BsrDI GCAATG_nn' Cuts at: 0 909 2217 Size: 909 1308 BsrGI T'GTAC_A Cuts at: 0 182 419 2217 Size: 182 237 1798 Fragments arranged by size: 1798 237 182 BssSI C'ACGA_G Cuts at: 0 1142 2217 Size: 1142 1075 BstAPI GCAn_nnn'nTGC Cuts at: 0 468 2217 Size: 468 1749 BstXI CCAn_nnnn'nTGG Cuts at: 0 123 1894 2118 2217 Size: 123 1771 224 99 Fragments arranged by size: 1771 224 123 99 BstYI r'GATC_y Cuts at: 0 93 602 875 1226 2217 Size: 93 509 273 351 991 Fragments arranged by size: 991 509 351 273 93 Bsu36I CC'TnA_GG Cuts at: 0 1180 2217 Size: 1180 1037 BtgI C'CryG_G Cuts at: 0 27 76 259 495 1925 2217 Size: 27 49 183 236 1430 292 Fragments arranged by size: 1430 292 236 183 49 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 359 1220 1661 2217 Size: 359 861 441 556 Fragments arranged by size: 861 556 441 359 BtsI GCAGTG_nn' Cuts at: 0 1204 1306 1580 2217 Size: 1204 102 274 637 Fragments arranged by size: 1204 637 274 102 ClaI AT'CG_AT Cuts at: 0 1856 2217 Size: 1856 361 DraIII CAC_nnn'GTG Cuts at: 0 180 2217 Size: 180 2037 DrdI GACnn_nn'nnGTC Cuts at: 0 2062 2217 Size: 2062 155 EaeI y'GGCC_r Cuts at: 0 30 561 779 967 1355 1421 1910 2012 Size: 30 531 218 188 388 66 489 102 Cuts at: 2012 2217 Size: 205 Fragments arranged by size: 531 489 388 218 205 188 102 66 30 EagI C'GGCC_G Cuts at: 0 30 967 2217 Size: 30 937 1250 Fragments arranged by size: 1250 937 30 EarI CTCTTCn'nnn_ Cuts at: 0 333 622 697 1607 2206 2217 Size: 333 289 75 910 599 11 Fragments arranged by size: 910 599 333 289 75 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 493 610 2217 Size: 493 117 1607 Fragments arranged by size: 1607 493 117 EcoICRI GAG'CTC Cuts at: 0 1956 2217 Size: 1956 261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 421 502 769 775 793 1018 1213 Size: 177 244 81 267 6 18 225 195 Cuts at: 1213 1268 1417 1615 1726 1735 1897 2217 Size: 55 149 198 111 9 162 320 Fragments arranged by size: 320 267 244 225 198 195 177 162 149 111 81 55 18 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1219 2065 2217 Size: 1219 846 152 Fragments arranged by size: 1219 846 152 EcoO109I rG'GnC_Cy Cuts at: 0 295 1155 2217 Size: 295 860 1062 Fragments arranged by size: 1062 860 295 HaeII r_GCGC'y Cuts at: 0 40 259 845 1464 2217 Size: 40 219 586 619 753 Fragments arranged by size: 753 619 586 219 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 712 744 991 1023 1264 1296 1639 1671 Size: 712 32 247 32 241 32 343 32 Cuts at: 1671 2217 Size: 546 Fragments arranged by size: 712 546 343 247 241 32 32 32 32 HincII GTy'rAC Cuts at: 0 2049 2217 Size: 2049 168 KasI G'GCGC_C Cuts at: 0 36 2217 Size: 36 2181 MscI TGG'CCA Cuts at: 0 781 2217 Size: 781 1436 MspA1I CmG'CkG Cuts at: 0 29 78 110 465 975 1191 1875 2217 Size: 29 49 32 355 510 216 684 342 Fragments arranged by size: 684 510 355 342 216 49 32 29 NarI GG'CG_CC Cuts at: 0 37 2217 Size: 37 2180 NcoI C'CATG_G Cuts at: 0 259 495 2217 Size: 259 236 1722 Fragments arranged by size: 1722 259 236 NruI TCG'CGA Cuts at: 0 344 2217 Size: 344 1873 NspI r_CATG'y Cuts at: 0 543 705 1152 1923 2217 Size: 543 162 447 771 294 Fragments arranged by size: 771 543 447 294 162 PasI CC'CwG_GG Cuts at: 0 123 456 1158 2217 Size: 123 333 702 1059 Fragments arranged by size: 1059 702 333 123 PciI A'CATG_T Cuts at: 0 701 2217 Size: 701 1516 PfoI T'CCnGG_A Cuts at: 0 791 878 1268 2217 Size: 791 87 390 949 Fragments arranged by size: 949 791 390 87 PpuMI rG'GwC_Cy Cuts at: 0 295 2217 Size: 295 1922 PspOMI G'GGCC_C Cuts at: 0 1154 2217 Size: 1154 1063 PstI C_TGCA'G Cuts at: 0 1127 1313 1412 1457 1562 1667 2217 Size: 1127 186 99 45 105 105 550 Fragments arranged by size: 1127 550 186 105 105 99 45 PvuII CAG'CTG Cuts at: 0 110 975 1191 1875 2217 Size: 110 865 216 684 342 Fragments arranged by size: 865 684 342 216 110 SacI G_AGCT'C Cuts at: 0 1958 2217 Size: 1958 259 SacII CC_GC'GG Cuts at: 0 30 79 2217 Size: 30 49 2138 Fragments arranged by size: 2138 49 30 SapI GCTCTTCn'nnn_ Cuts at: 0 697 2206 2217 Size: 697 1509 11 Fragments arranged by size: 1509 697 11 SfcI C'TryA_G Cuts at: 0 1003 1123 1309 1408 1453 1558 1663 1734 Size: 1003 120 186 99 45 105 105 71 Cuts at: 1734 2217 Size: 483 Fragments arranged by size: 1003 483 186 120 105 105 99 71 45 SfoI GGC'GCC Cuts at: 0 38 2217 Size: 38 2179 SmaI CCC'GGG Cuts at: 0 1255 2217 Size: 1255 962 SmlI C'TyrA_G Cuts at: 0 370 733 1297 2217 Size: 370 363 564 920 Fragments arranged by size: 920 564 370 363 SphI G_CATG'C Cuts at: 0 543 1152 1923 2217 Size: 543 609 771 294 Fragments arranged by size: 771 609 543 294 StyI C'CwwG_G Cuts at: 0 259 495 833 1688 2217 Size: 259 236 338 855 529 Fragments arranged by size: 855 529 338 259 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1540 2217 Size: 1540 677 TatI w'GTAC_w Cuts at: 0 182 419 2217 Size: 182 237 1798 Fragments arranged by size: 1798 237 182 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1266 1298 2217 Size: 1266 32 919 Fragments arranged by size: 1266 919 32 Tth111I GACn'n_nGTC Cuts at: 0 726 1552 2062 2217 Size: 726 826 510 155 Fragments arranged by size: 826 726 510 155 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 807 2115 2217 Size: 807 1308 102 Fragments arranged by size: 1308 807 102 XmaI C'CCGG_G Cuts at: 0 1253 2217 Size: 1253 964 XmnI GAAnn'nnTTC Cuts at: 0 691 2096 2217 Size: 691 1405 121 Fragments arranged by size: 1405 691 121 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MscI MspA1I NarI NcoI NruI NspI PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I CspCI DraI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV NheI NotI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI ZraI