################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HgaI HincII HindII HindIII HpaI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RcaI SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 846 GGTACC G^GTACC AcyI 73 GGCGCC G[GA]^CG[CT]C AflIII 168 ACGTGT A^C[GA][CT]GT AocI 957 CCTAAGG CC^T[ACGT]AGG ApaI 1318 GGGCCC GGGCC^C AseI 1396 ATTAAT AT^TAAT AsnI 1396 ATTAAT AT^TAAT Asp700I 790 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 846 GGTACC G^GTACC BamHI 425 GGATCC G^GATCC BbeI 73 GGCGCC GGCGC^C BciVI 1268 GTATCC GTATCC(6/5) BclI 1464 TGATCA T^GATCA BglI 76 GCCCCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 167 TACGTG [CT]AC^GT[GA] BsaHI 73 GGCGCC G[GA]^CG[CT]C BsaWI 9 TCCGGT [AT]^CCGG[AT] BseRI 40 GAGGAG GAGGAG(10/8) BsmFI 625 GGGAC GGGAC(10/14) BsmI 255 GCATTC GAATGC(1/-1) BspMI 1122 ACCTGC ACCTGC(4/8) BsrBI 92 CCGCTC CCGCTC(-3/-3) Bsu36I 957 CCTAAGG CC^T[ACGT]AGG CfoI 74 GCGC GCG^C CpoI 50 CGGACCG CG^G[AT]CCG DraI 855 TTTAAA TTT^AAA EaeI 1172 TGGCCG [CT]^GGCC[GA] Eco81I 957 CCTAAGG CC^T[ACGT]AGG EcoNI 1000 CCTTATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1464 TGATCA T^GATCA HaeII 73 GGCGCC [GA]GCGC^[CT] HhaI 74 GCGC GCG^C Hin1I 73 GGCGCC G[GA]^CG[CT]C HinP1I 74 GCGC G^CGC KasI 73 GGCGCC G^GCGCC KpnI 846 GGTACC GGTAC^C MaeII 168 ACGT A^CGT NarI 73 GGCGCC GG^CGCC NcoI 1470 CCATGG C^CATGG NdeI 605 CATATG CA^TATG PshAI 678 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1396 ATTAAT AT^TAAT RsrII 50 CGGACCG CG^G[AT]CCG SapI 1217 GCTCTTC GCTCTTC(1/4) SfoI 73 GGCGCC GGC^GCC SmlI 320 CTCAAG C^T[CT][GA]AG SphI 546 GCATGC GCATG^C SspI 711 AATATT AAT^ATT TaqI 15 TCGA T^CGA Tsp45I 746 GTGAC ^GT[GC]AC TthHB8I 15 TCGA T^CGA XmnI 790 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 54 CCGC CCGC(-3/-1) 92 CCGC BbsI 558 GTCTTC GAAGAC(2/6) 685 GTCTTC BcnI 26 CCCGG CC^[GC]GG 70 CCGGG BpmI 95 CTCCAG CTGGAG(16/14) 374 CTGGAG BpuAI 558 GTCTTC GAAGAC(2/6) 685 GTCTTC BsaI 17 GAGACC GGTCTC(1/5) 5 GGTCTC BsiYI 643 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1000 CCTTATTTAGG BslI 643 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1000 CCTTATTTAGG BsmAI 17 GAGAC GTCTC(1/5) 6 GTCTC BstF5I 309 GGATG GGATG(2/0) 325 GGATG EcoT22I 161 ATGCAT ATGCA^T 544 ATGCAT FokI 309 GGATG GGATG(9/13) 325 GGATG NciI 26 CCCGG CC^[GC]GG 70 CCGGG NsiI 161 ATGCAT ATGCA^T 544 ATGCAT NspI 244 GCATGT [GA]CATG^[CT] 546 GCATGC PstI 467 CTGCAG CTGCA^G 1124 CTGCAG SfcI 467 CTGCAG C^T[GA][CT]AG 1124 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbvI BfaI BmyI BsaJI BsiHKAI Bsp1286I BsrDI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII