(Circular) (Six-base) MAPSORT of: pFN21AE4476.seq Check: 4585 from: 1 to: 6190 pFN21AE4476 6190 bp With 182 enzymes: SgfI * March 28, 2012 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 2851 3511 2851 Size: 660 5530 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4253 5637 5905 1632 Size: 2621 1384 268 1917 Fragments arranged by size: 2621 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 2173 3487 5089 6066 1203 Size: 970 1314 1602 977 1327 Fragments arranged by size: 1602 1327 1314 977 970 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 2326 Size: 32 6158 AlwNI CAG_nnn'CTG Cuts at: 3933 5505 3933 Size: 1572 4618 ApaI G_GGCC'C Cuts at: 3327 3327 Size: 6190 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 2775 3073 3099 3499 3725 3877 5986 1493 Size: 1282 298 26 400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1282 400 298 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 3402 4744 160 Size: 872 2370 1342 1606 Fragments arranged by size: 2370 1606 1342 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 3515 1870 Size: 73 1572 4545 Fragments arranged by size: 4545 1572 73 BamHI G'GATC_C Cuts at: 2430 3520 3887 2430 Size: 1090 367 4733 Fragments arranged by size: 4733 1090 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2851 3413 3511 4966 Size: 325 217 627 291 773 562 98 1455 Cuts at: 4966 618 Size: 1842 Fragments arranged by size: 1842 1455 773 627 562 325 291 217 98 BanII G_rGCy'C Cuts at: 1391 2575 3327 3463 3509 1391 Size: 1184 752 136 46 4072 Fragments arranged by size: 4072 1184 752 136 46 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 6190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 2683 928 Size: 971 657 127 4435 Fragments arranged by size: 4435 971 657 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4082 5292 3284 Size: 798 1210 4182 Fragments arranged by size: 4182 1210 798 BclI T'GATC_A Cuts at: 1548 3469 1548 Size: 1921 4269 BfrBI ATG'CAT Cuts at: 2168 2551 2168 Size: 383 5807 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2087 4801 136 Size: 107 122 71 631 605 415 2714 1525 Fragments arranged by size: 2714 1525 631 605 415 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 3607 3607 Size: 6190 Bme1580I G_kGCm'C Cuts at: 1790 3327 4251 5407 1790 Size: 1537 924 1156 2573 Fragments arranged by size: 2573 1537 1156 924 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3218 3466 3921 Size: 416 53 83 399 24 1801 248 455 Cuts at: 3921 4879 442 Size: 958 1753 Fragments arranged by size: 1801 1753 958 455 416 399 248 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2400 4850 1239 Size: 846 315 2450 2579 Fragments arranged by size: 2579 2450 846 315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2310 4315 5180 5478 2310 Size: 2005 865 298 3022 Fragments arranged by size: 3022 2005 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2016 4853 882 Size: 617 517 2837 2219 Fragments arranged by size: 2837 2219 617 517 BsaAI yAC'GTr Cuts at: 493 1204 2174 3981 493 Size: 711 970 1807 2702 Fragments arranged by size: 2702 1807 970 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3997 4379 5014 Size: 53 83 186 936 546 1918 382 635 Cuts at: 5014 275 Size: 1451 Fragments arranged by size: 1918 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2014 4623 5021 5295 5442 5853 2014 Size: 2609 398 274 147 411 2351 Fragments arranged by size: 2609 2351 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2373 2403 2517 2547 1178 Size: 30 165 30 970 30 114 30 4821 Fragments arranged by size: 4821 970 165 114 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 1186 Size: 874 5316 BseYI C'CCAG_C Cuts at: 5393 5889 5393 Size: 496 5694 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5617 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2512 2623 4251 4336 5407 2054 Size: 458 111 1628 85 1071 2837 Fragments arranged by size: 2837 1628 1071 458 111 85 BsmI GAATG_Cn' Cuts at: 2260 3701 3794 2260 Size: 1441 93 4656 Fragments arranged by size: 4656 1441 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2512 2575 2623 3327 3463 3509 Size: 399 264 458 63 48 704 136 46 Cuts at: 3509 4251 4336 5407 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 458 399 264 136 85 63 48 46 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3136 3545 844 Size: 2292 409 3489 Fragments arranged by size: 3489 2292 409 BsrBI CCG'CTC Cuts at: 1394 1823 2099 4077 1394 Size: 429 276 1978 3507 Fragments arranged by size: 3507 1978 429 276 BsrDI GCAATG_nn' Cuts at: 65 1248 2466 2999 3330 4685 4859 65 Size: 1183 1218 533 331 1355 174 1396 Fragments arranged by size: 1396 1355 1218 1183 533 331 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4834 5021 1700 Size: 110 3024 187 2869 Fragments arranged by size: 3024 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2430 2736 2958 3520 3887 4272 4289 5741 Size: 458 306 222 562 367 385 17 1452 Cuts at: 5741 5827 5839 6185 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 562 458 385 367 346 306 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2963 2963 Size: 6190 BtgI C'CryG_G Cuts at: 513 1065 2084 2846 3475 513 Size: 552 1019 762 629 3228 Fragments arranged by size: 3228 1019 762 629 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 2439 3706 4500 4520 2439 Size: 1267 794 20 4109 Fragments arranged by size: 4109 1267 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 2862 3494 2862 Size: 632 5558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3177 3637 4526 Size: 54 1425 50 275 18 1347 460 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1347 889 460 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 2649 3229 4120 1353 Size: 720 576 580 891 3423 Fragments arranged by size: 3423 891 720 580 576 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4777 5151 5297 387 Size: 1064 138 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2400 4253 4850 5637 5905 1239 Size: 393 453 315 1853 597 787 268 1524 Fragments arranged by size: 1853 1524 787 597 453 393 315 268 EcoNI CCTnn'n_nnAGG Cuts at: 3009 3009 Size: 6190 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 2082 5337 1678 Size: 404 3255 2531 Fragments arranged by size: 3255 2531 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2322 2354 2415 2447 3222 Size: 32 135 32 750 32 61 32 775 Cuts at: 3222 3254 4831 4863 4905 4937 1373 Size: 32 1577 32 42 32 2626 Fragments arranged by size: 2626 1577 775 750 135 61 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KasI G'GCGC_C Cuts at: 2078 2078 Size: 6190 KpnI G_GTAC'C Cuts at: 2855 3515 2855 Size: 660 5530 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2467 3343 5304 5488 1305 Size: 1162 876 1961 184 2007 Fragments arranged by size: 2007 1961 1162 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4368 Fragments arranged by size: 4368 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4148 4507 4666 518 Size: 648 2982 359 159 2042 Fragments arranged by size: 2982 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3606 4283 5431 5676 1606 Size: 2000 677 1148 245 2120 Fragments arranged by size: 2120 2000 1148 677 245 NarI GG'CG_CC Cuts at: 2079 2079 Size: 6190 NcoI C'CATG_G Cuts at: 513 1065 3475 513 Size: 552 2410 3228 Fragments arranged by size: 3228 2410 552 NdeI CA'TA_TG Cuts at: 387 2611 387 Size: 2224 3966 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NsiI A_TGCA'T Cuts at: 2170 2553 2170 Size: 383 5807 NspI r_CATG'y Cuts at: 1132 1229 2253 2555 3548 5093 1132 Size: 97 1024 302 993 1545 2229 Fragments arranged by size: 2229 1545 1024 993 302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6190 PfoI T'CCnGG_A Cuts at: 2377 3310 2377 Size: 933 5257 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2687 1613 Size: 1074 5116 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 3323 3323 Size: 6190 PstI C_TGCA'G Cuts at: 830 2476 3133 3542 830 Size: 1646 657 409 3478 Fragments arranged by size: 3478 1646 657 409 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 RsrII CG'GwC_CG Cuts at: 2056 2056 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 3229 3229 Size: 6190 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2472 3129 3538 4673 5354 5545 826 Size: 219 1427 657 409 1135 681 191 1471 Fragments arranged by size: 1471 1427 1135 681 657 409 219 191 SfoI GGC'GCC Cuts at: 2080 2080 Size: 6190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2325 4294 5195 5457 820 Size: 197 926 382 1969 901 262 1553 Fragments arranged by size: 1969 1553 926 901 382 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 2555 3548 1132 Size: 1423 993 3774 Fragments arranged by size: 3774 1423 993 SspI AAT'ATT Cuts at: 5 52 2718 4114 5 Size: 47 2666 1396 2081 Fragments arranged by size: 2666 2081 1396 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2244 2434 3475 3629 513 Size: 552 1179 190 1041 154 3074 Fragments arranged by size: 3074 1179 1041 552 190 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2500 3448 4436 Size: 274 80 33 51 1443 523 948 988 Cuts at: 4436 6148 96 Size: 1712 138 Fragments arranged by size: 1712 1443 988 948 523 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2359 2391 6150 6182 2359 Size: 32 3759 32 2367 Fragments arranged by size: 3759 2367 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 2799 4319 2799 Size: 1520 4670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI