(Linear) (Six-base) MAPSORT of: KIEE4476.seq Check: 2033 from: 1 to: 1485 KIEE4476.orf With 182 enzymes: SgfI * March 22, 2012 18:09 .. Acc65I G'GTAC_C Cuts at: 0 846 1485 Size: 846 639 AflIII A'CryG_T Cuts at: 0 168 1485 Size: 168 1317 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 321 353 1485 Size: 321 32 1132 Fragments arranged by size: 1132 321 32 ApaI G_GGCC'C Cuts at: 0 1322 1485 Size: 1322 163 ApoI r'AATT_y Cuts at: 0 770 1068 1094 1485 Size: 770 298 26 391 Fragments arranged by size: 770 391 298 26 AseI AT'TA_AT Cuts at: 0 1397 1485 Size: 1397 88 BamHI G'GATC_C Cuts at: 0 425 1485 Size: 425 1060 BanI G'GyrC_C Cuts at: 0 73 846 1408 1485 Size: 73 773 562 77 Fragments arranged by size: 773 562 77 73 BanII G_rGCy'C Cuts at: 0 570 1322 1458 1485 Size: 570 752 136 27 Fragments arranged by size: 752 570 136 27 BbeI G_GCGC'C Cuts at: 0 77 1485 Size: 77 1408 BbsI GAAGACnn'nnnn_ Cuts at: 0 551 678 1485 Size: 551 127 807 Fragments arranged by size: 807 551 127 BciVI GTATCCnnnnn_n' Cuts at: 0 1279 1485 Size: 1279 206 BclI T'GATC_A Cuts at: 0 1464 1485 Size: 1464 21 BfrBI ATG'CAT Cuts at: 0 163 546 1485 Size: 163 383 939 Fragments arranged by size: 939 383 163 BglI GCCn_nnn'nGGC Cuts at: 0 82 1485 Size: 82 1403 Bme1580I G_kGCm'C Cuts at: 0 1322 1485 Size: 1322 163 BmrI ACTGGGnnnn_n' Cuts at: 0 1213 1461 1485 Size: 1213 248 24 Fragments arranged by size: 1213 248 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 395 1485 Size: 80 315 1090 Fragments arranged by size: 1090 315 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 1485 Size: 305 1180 BsaI GGTCTCn'nnnn_ Cuts at: 0 11 1485 Size: 11 1474 BsaAI yAC'GTr Cuts at: 0 169 1485 Size: 169 1316 BsaHI Gr'CG_yC Cuts at: 0 74 1485 Size: 74 1411 BsaWI w'CCGG_w Cuts at: 0 9 1485 Size: 9 1476 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 368 398 512 542 1485 Size: 368 30 114 30 943 Fragments arranged by size: 943 368 114 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 1485 Size: 55 1430 BsiHKAI G_wGCw'C Cuts at: 0 49 507 618 1485 Size: 49 458 111 867 Fragments arranged by size: 867 458 111 49 BsmI GAATG_Cn' Cuts at: 0 255 1485 Size: 255 1230 Bsp1286I G_dGCh'C Cuts at: 0 49 507 570 618 1322 1458 1485 Size: 49 458 63 48 704 136 27 Fragments arranged by size: 704 458 136 63 49 48 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1131 1485 Size: 1131 354 BsrBI CCG'CTC Cuts at: 0 94 1485 Size: 94 1391 BsrDI GCAATG_nn' Cuts at: 0 461 994 1325 1485 Size: 461 533 331 160 Fragments arranged by size: 533 461 331 160 BstYI r'GATC_y Cuts at: 0 425 731 953 1485 Size: 425 306 222 532 Fragments arranged by size: 532 425 306 222 Bsu36I CC'TnA_GG Cuts at: 0 958 1485 Size: 958 527 BtgI C'CryG_G Cuts at: 0 79 841 1470 1485 Size: 79 762 629 15 Fragments arranged by size: 762 629 79 15 BtsI GCAGTG_nn' Cuts at: 0 434 1485 Size: 434 1051 DraI TTT'AAA Cuts at: 0 857 1485 Size: 857 628 EaeI y'GGCC_r Cuts at: 0 1172 1485 Size: 1172 313 EarI CTCTTCn'nnn_ Cuts at: 0 68 644 1224 1485 Size: 68 576 580 261 Fragments arranged by size: 580 576 261 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 395 1485 Size: 80 315 1090 Fragments arranged by size: 1090 315 80 EcoNI CCTnn'n_nnAGG Cuts at: 0 1004 1485 Size: 1004 481 HaeII r_GCGC'y Cuts at: 0 77 1485 Size: 77 1408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 317 349 410 442 1217 1249 1485 Size: 317 32 61 32 775 32 236 Fragments arranged by size: 775 317 236 61 32 32 32 KasI G'GCGC_C Cuts at: 0 73 1485 Size: 73 1412 KpnI G_GTAC'C Cuts at: 0 850 1485 Size: 850 635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 462 1338 1485 Size: 462 876 147 Fragments arranged by size: 876 462 147 NarI GG'CG_CC Cuts at: 0 74 1485 Size: 74 1411 NcoI C'CATG_G Cuts at: 0 1470 1485 Size: 1470 15 NdeI CA'TA_TG Cuts at: 0 606 1485 Size: 606 879 NsiI A_TGCA'T Cuts at: 0 165 548 1485 Size: 165 383 937 Fragments arranged by size: 937 383 165 NspI r_CATG'y Cuts at: 0 248 550 1485 Size: 248 302 935 Fragments arranged by size: 935 302 248 PfoI T'CCnGG_A Cuts at: 0 372 1305 1485 Size: 372 933 180 Fragments arranged by size: 933 372 180 PshAI GACnn'nnGTC Cuts at: 0 682 1485 Size: 682 803 PspOMI G'GGCC_C Cuts at: 0 1318 1485 Size: 1318 167 PstI C_TGCA'G Cuts at: 0 471 1128 1485 Size: 471 657 357 Fragments arranged by size: 657 471 357 RsrII CG'GwC_CG Cuts at: 0 51 1485 Size: 51 1434 SapI GCTCTTCn'nnn_ Cuts at: 0 1224 1485 Size: 1224 261 SfcI C'TryA_G Cuts at: 0 467 1124 1485 Size: 467 657 361 Fragments arranged by size: 657 467 361 SfoI GGC'GCC Cuts at: 0 75 1485 Size: 75 1410 SmlI C'TyrA_G Cuts at: 0 320 1485 Size: 320 1165 SphI G_CATG'C Cuts at: 0 550 1485 Size: 550 935 SspI AAT'ATT Cuts at: 0 713 1485 Size: 713 772 StyI C'CwwG_G Cuts at: 0 239 429 1470 1485 Size: 239 190 1041 15 Fragments arranged by size: 1041 239 190 15 TatI w'GTAC_w Cuts at: 0 495 1443 1485 Size: 495 948 42 Fragments arranged by size: 948 495 42 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 354 386 1485 Size: 354 32 1099 Fragments arranged by size: 1099 354 32 XmnI GAAnn'nnTTC Cuts at: 0 794 1485 Size: 794 691 Enzymes that do cut: Acc65I AflIII AloI ApaI ApoI AseI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstYI Bsu36I BtgI BtsI DraI EaeI EarI Eco57MI EcoNI HaeII Hin4I KasI KpnI MmeI NarI NcoI NdeI NsiI NspI PfoI PshAI PspOMI PstI RsrII SapI SfcI SfoI SmlI SphI SspI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AlwNI ApaLI AscI AsiSI AvaI AvrII BaeI BbvCI BcgI BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MscI MslI MspA1I NaeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI