################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 879 CCGC CCGC(-3/-1) AcsI 357 GAATTT [GA]^AATT[CT] AlwNI 337 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 357 GAATTT [GA]^AATT[CT] AseI 133 ATTAAT AT^TAAT AsnI 133 ATTAAT AT^TAAT Asp700I 192 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 658 GAAGAC GAAGAC(2/6) BglII 1165 AGATCT A^GATCT BmyI 703 GTGCCC G[AGT]GC[ACT]^C BpuAI 658 GAAGAC GAAGAC(2/6) BsaWI 161 ACCGGA [AT]^CCGG[AT] Bsp1286I 703 GTGCCC G[AGT]GC[ACT]^C Cfr13I 248 GGGCC G^G[ACGT]CC DraI 616 TTTAAA TTT^AAA DraII 247 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 736 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 400 CTCTTC CTCTTC(1/4) EcoO109I 247 GGGGCCT [GA]G^G[ACGT]CC[CT] HaeIII 249 GGCC GG^CC HapII 162 CCGG C^CGG HincII 901 GTTGAC GT[CT]^[GA]AC HindII 901 GTTGAC GT[CT]^[GA]AC HpaII 162 CCGG C^CGG Ksp632I 400 CTCTTC CTCTTC(1/4) MspA1I 1094 CAGCTG C[AC]G^C[GT]G MspI 162 CCGG C^CGG NdeI 938 CATATG CA^TATG NspI 978 GCATGC [GA]CATG^[CT] PshBI 133 ATTAAT AT^TAAT PvuII 1094 CAGCTG CAG^CTG Sau96I 248 GGGCC G^G[ACGT]CC SmlI 574 CTCAAG C^T[CT][GA]AG SphI 978 GCATGC GCATG^C Tsp45I 3 GTCAC ^GT[GC]AC XmnI 192 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 31 GTAC GT^AC 159 GTAC BfaI 739 CTAG C^TAG 1216 CTAG BglI 622 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1048 GCCCATAAGGC BsaI 178 GAGACC GGTCTC(1/5) 280 GAGACC BsgI 759 CTGCAC GTGCAG(16/14) 1063 CTGCAC BsiYI 12 CCTGTATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 814 CCCAAGGAGGG BslI 12 CCTGTATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 814 CCCAAGGAGGG BsmFI 822 GGGAC GGGAC(10/14) 1099 GTCCC BstYI 320 AGATCC [GA]^GATC[CT] 1165 AGATCT DdeI 269 CTGAG C^T[ACGT]AG 597 CTTAG HinfI 832 GAGTC G^A[ACGT]TC 1010 GACTC MaeI 739 CTAG C^TAG 1216 CTAG MaeIII 3 GTCAC ^GT[ACGT]AC 79 GTAAC MflI 320 AGATCC [GA]^GATC[CT] 1165 AGATCT PleI 1010 GACTC GAGTC(4/5) 832 GAGTC PshAI 465 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1021 GACAATGGTC RsaI 31 GTAC GT^AC 159 GTAC XhoII 320 AGATCC [GA]^GATC[CT] 1165 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsmAI BsmI BsrI BstF5I Cac8I DpnII EcoT14I Fnu4HI FokI HphI ItaI MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV PstI Sau3AI SfaNI SfcI StyI Tru9I TseI Tsp509I TspRI