################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 294 GTCTAC GT^[AC][GT]AC AfaI 1359 GTAC GT^AC AflIII 777 ACATGT A^C[GA][CT]GT Alw44I 859 GTGCAC G^TGCAC ApaLI 859 GTGCAC G^TGCAC Asp700I 1090 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 859 GTGCAC G[AT]GC[AT]^C AvaI 1228 CCCGAG C^[CT]CG[GA]G BalI 114 TGGCCA TGG^CCA BanII 972 GAGCCC G[GA]GC[CT]^C BbrPI 1253 CACGTG CAC^GTG BcgI 1007 CGAGGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 867 TGATCA T^GATCA BsaI 1154 GAGACC GGTCTC(1/5) BseRI 384 GAGGAG GAGGAG(10/8) BsiHKAI 859 GTGCAC G[AT]GC[AT]^C BsoBI 1228 CCCGAG C^[CT]CG[GA]G Bsp1407I 1358 TGTACA T^GTACA BspLU11I 777 ACATGT A^CATGT BsrGI 1358 TGTACA T^GTACA BssSI 314 CACGAG CACGAG(-5/-1) BstEII 375 GGTTACC G^GT[ACGT]ACC BstPI 375 GGTTACC G^GT[ACGT]ACC BstXI 342 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 708 GCTGGC GC[ACGT]^[ACGT]GC DraI 717 TTTAAA TTT^AAA DraIII 131 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 924 CCATGG C^C[GA][CT]GG EaeI 114 TGGCCA [CT]^GGCC[GA] EcoO65I 375 GGTTACC G^GT[ACGT]ACC FbaI 867 TGATCA T^GATCA HindIII 1410 AAGCTT A^AGCTT MluNI 114 TGGCCA TGG^CCA MscI 114 TGGCCA TGG^CCA NcoI 924 CCATGG C^CATGG PmaCI 1253 CACGTG CAC^GTG PmlI 1253 CACGTG CAC^GTG PstI 19 CTGCAG CTGCA^G RsaI 1359 GTAC GT^AC SfaNI 101 GCATC GCATC(5/9) SnaBI 297 TACGTA TAC^GTA SspBI 1358 TGTACA T^GTACA StuI 1379 AGGCCT AGG^CCT SwaI 716 ATTTAAAT ATTT^AAAT TfiI 361 GATTC G^A[AT]TC XmnI 1090 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 424 GAATTT [GA]^AATT[CT] 490 AAATTT ApoI 424 GAATTT [GA]^AATT[CT] 490 AAATTT BbsI 691 GAAGAC GAAGAC(2/6) 160 GTCTTC BmyI 859 GTGCAC G[AGT]GC[ACT]^C 972 GAGCCC BpuAI 691 GAAGAC GAAGAC(2/6) 160 GTCTTC BsaAI 297 TACGTA [CT]AC^GT[GA] 1253 CACGTG BsmI 999 GCATTC GAATGC(1/-1) 1050 GCATTC Bsp1286I 859 GTGCAC G[AGT]GC[ACT]^C 972 GAGCCC DraII 726 GGGACCC [GA]G^G[ACGT]CC[CT] 1009 AGGACCT EarI 827 GAAGAG CTCTTC(1/4) 961 GAAGAG EcoO109I 726 GGGACCC [GA]G^G[ACGT]CC[CT] 1009 AGGACCT HaeII 15 GGCGCT [GA]GCGC^[CT] 33 GGCGCT HgaI 199 GACGC GACGC(5/10) 671 GACGC Ksp632I 827 GAAGAG CTCTTC(1/4) 961 GAAGAG MaeII 298 ACGT A^CGT 1254 ACGT MseI 718 TTAA T^TAA 1069 TTAA MslI 102 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1267 CATTTGGATG NspI 674 GCATGT [GA]CATG^[CT] 777 ACATGT PpuMI 726 GGGACCC [GA]G^G[AT]CC[CT] 1009 AGGACCT SfcI 19 CTGCAG C^T[GA][CT]AG 1383 CTGTAG Tru9I 718 TTAA T^TAA 1069 TTAA Tsp45I 576 GTCAC ^GT[GC]AC 1223 GTGAC XcmI 146 CCACTTTTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 698 CCAGGCGGCGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BfaI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspI MvaI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI TaqI TseI Tsp509I TspRI TthHB8I XhoII