(Circular) (Six-base) MAPSORT of: pFN21AE1511.seq Check: 4129 from: 1 to: 5671 pFN21AE1511 5671 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 2628 3734 5118 5386 1632 Size: 839 157 1106 1384 268 1917 Fragments arranged by size: 1917 1384 1106 839 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AlwNI CAG_nnn'CTG Cuts at: 2735 3414 4986 2735 Size: 679 1572 3420 Fragments arranged by size: 3420 1572 679 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2925 2996 1870 Size: 73 982 71 4545 Fragments arranged by size: 4545 982 73 71 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2992 4447 618 Size: 325 217 627 1205 1455 1842 Fragments arranged by size: 1842 1455 1205 627 325 217 BanII G_rGCy'C Cuts at: 1391 2044 2990 1391 Size: 653 946 4072 Fragments arranged by size: 4072 946 653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 928 Size: 971 962 3738 Fragments arranged by size: 3738 971 962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2728 2762 3862 3896 1903 Size: 34 791 34 1100 34 3678 Fragments arranged by size: 3678 1100 791 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4282 136 Size: 107 122 71 631 605 2610 1525 Fragments arranged by size: 2610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 3732 4888 1790 Size: 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 BmgBI CAC'GTC Cuts at: 1318 2548 1318 Size: 1230 4441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3402 4360 442 Size: 416 53 83 399 24 1985 958 1753 Fragments arranged by size: 1985 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 2792 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4331 1239 Size: 3092 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 2560 3796 4661 4959 2135 Size: 425 1236 865 298 2847 Fragments arranged by size: 2847 1236 865 425 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2295 2780 4334 882 Size: 617 796 485 1554 2219 Fragments arranged by size: 2219 1554 796 617 485 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 1186 Size: 896 4775 BseYI C'CCAG_C Cuts at: 2293 4874 5370 2293 Size: 2581 496 2594 Fragments arranged by size: 2594 2581 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2044 3732 3817 4888 2044 Size: 1688 85 1071 2827 Fragments arranged by size: 2827 1688 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 BsmBI CGTCTCn'nnnn_ Cuts at: 2538 2538 Size: 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2990 3732 3817 4888 1391 Size: 399 254 946 742 85 1071 2174 Fragments arranged by size: 2174 1071 946 742 399 254 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 3026 844 Size: 1325 857 3489 Fragments arranged by size: 3489 1325 857 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 2064 3731 4743 2064 Size: 1667 1012 2992 Fragments arranged by size: 2992 1667 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstYI r'GATC_y Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666 Size: 1029 367 385 17 1452 86 12 346 Cuts at: 5666 1972 Size: 1977 Fragments arranged by size: 1977 1452 1029 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2967 2967 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 3187 3981 4001 3187 Size: 794 20 4857 Fragments arranged by size: 4857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2202 2237 529 Size: 35 1638 35 3963 Fragments arranged by size: 3963 1638 35 35 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2332 3118 4007 Size: 54 1425 50 275 18 502 786 889 Cuts at: 4007 5636 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 786 502 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 4258 4632 4778 387 Size: 1064 138 724 1945 374 146 1280 Fragments arranged by size: 1945 1280 1064 724 374 146 138 EcoICRI GAG'CTC Cuts at: 2042 2042 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2628 3734 4331 5118 5386 1239 Size: 393 839 157 1106 597 787 268 1524 Fragments arranged by size: 1524 1106 839 787 597 393 268 157 EcoNI CCTnn'n_nnAGG Cuts at: 2450 2450 Size: 5671 EcoO109I rG'GnC_Cy Cuts at: 2084 2158 2084 Size: 74 5597 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 EcoRV GAT'ATC Cuts at: 2389 2389 Size: 5671 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2013 4818 1678 Size: 335 2805 2531 Fragments arranged by size: 2805 2531 335 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373 Size: 32 135 32 2740 32 42 32 2626 Fragments arranged by size: 2740 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2704 3015 3261 669 Size: 988 281 766 311 246 3079 Fragments arranged by size: 3079 988 766 311 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 2316 3270 2316 Size: 954 4717 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2334 10 Size: 54 1425 343 502 3347 Fragments arranged by size: 3347 1425 502 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 3629 3988 4147 518 Size: 648 1203 1260 359 159 2042 Fragments arranged by size: 2042 1260 1203 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2242 3087 3764 4912 5157 1606 Size: 636 845 677 1148 245 2120 Fragments arranged by size: 2120 1148 845 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5119 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 3029 4574 1132 Size: 97 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 97 PasI CC'CwG_GG Cuts at: 1348 2555 2848 1348 Size: 1207 293 4171 Fragments arranged by size: 4171 1207 293 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 2735 1108 Size: 1203 424 4044 Fragments arranged by size: 4044 1203 424 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2158 Size: 5671 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 3023 830 Size: 2193 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2242 2242 Size: 5671 SacI G_AGCT'C Cuts at: 2044 2044 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2088 3019 4154 4835 5026 826 Size: 219 1043 931 1135 681 191 1471 Fragments arranged by size: 1471 1135 1043 931 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 2539 3775 4676 4938 820 Size: 197 926 171 425 1236 901 262 1553 Fragments arranged by size: 1553 1236 926 901 425 262 197 171 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2213 2517 2904 3110 513 Size: 552 1148 304 387 206 3074 Fragments arranged by size: 3074 1148 552 387 304 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 2485 3698 3715 3868 4053 4498 Size: 1255 779 126 1213 17 153 185 445 Cuts at: 4498 325 Size: 1498 Fragments arranged by size: 1498 1255 1213 779 445 185 153 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2354 2490 2354 Size: 136 5535 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI