################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MluI MluNI MroI MscI MslI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 205 GTAC GT^AC AlwI 195 GGATC GGATC(4/5) AlwNI 299 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 171 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 294 GAGCTC G[AT]GC[AT]^C AspI 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 210 GGTCC G^G[AT]CC BbsI 79 GAAGAC GAAGAC(2/6) BglII 266 AGATCT A^GATCT BlpI 296 GCTCAGC GC^T[ACGT]AGC BpmI 499 CTGGAG CTGGAG(16/14) Bpu1102I 296 GCTCAGC GC^T[ACGT]AGC BpuAI 79 GAAGAC GAAGAC(2/6) BsaI 336 GGTCTC GGTCTC(1/5) BsgI 531 CTGCAC GTGCAG(16/14) BsiHKAI 294 GAGCTC G[AT]GC[AT]^C BsmI 182 GCATTC GAATGC(1/-1) BspHI 186 TCATGA T^CATGA BsrFI 452 GCCGGC [GA]^CCGG[CT] BstAPI 533 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 266 AGATCT [GA]^GATC[CT] CelII 296 GCTCAGC GC^T[ACGT]AGC Cfr10I 452 GCCGGC [GA]^CCGG[CT] FauI 121 CCCGC CCCGC(4/6) MfeI 543 CAATTG C^AATTG MflI 266 AGATCT [GA]^GATC[CT] MseI 163 TTAA T^TAA MspA1I 400 CAGCTG C[AC]G^C[GT]G MunI 543 CAATTG C^AATTG MwoI 533 GCACCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 452 GCCGGC GCC^GGC NgoMIV 452 GCCGGC G^CCGGC NspI 494 ACATGC [GA]CATG^[CT] PflFI 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 400 CAGCTG CAG^CTG RcaI 186 TCATGA T^CATGA RsaI 205 GTAC GT^AC SacI 294 GAGCTC GAGCT^C ScaI 204 AGTACT AGT^ACT SmlI 291 CTTGAG C^T[CT][GA]AG Tru9I 163 TTAA T^TAA Tth111I 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 266 AGATCT [GA]^GATC[CT] XmnI 171 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbvI 71 GCTGC GCAGC(8/12) 430 GCTGC BcnI 84 CCCGG CC^[GC]GG 517 CCCGG BsiYI 5 CCTTTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 636 CCCCAGTGAGG BslI 5 CCTTTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 636 CCCCAGTGAGG BsmAI 337 GTCTC GTCTC(1/5) 356 GTCTC DpnII 196 GATC ^GATC 267 GATC HaeIII 109 GGCC GG^CC 455 GGCC MaeIII 365 GTGAC ^GT[ACGT]AC 565 GTGAC MboI 196 GATC ^GATC 267 GATC NciI 84 CCCGG CC^[GC]GG 517 CCCGG NdeII 196 GATC ^GATC 267 GATC NlaIV 102 GGAACC GG[ACGT]^[ACGT]CC 484 GGCTCC PleI 217 GACTC GAGTC(4/5) 502 GAGTC Sau3AI 196 GATC ^GATC 267 GATC TseI 71 GCTGC G^C[AT]GC 430 GCTGC Tsp45I 365 GTGAC ^GT[GC]AC 565 GTGAC TspRI 67 CAGTG CA[GC]TG[ACGT][ACGT]^ 639 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AcsI AluI ApoI BanII BmyI BsaJI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MboII MnlI MspI MvaI NlaIII Sau96I ScrFI TfiI Tsp509I