################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AlwNI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraI DraII DrdI Eam1105I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MboI MfeI MflI MluI MseI MunI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XcmI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 907 GTCTAC GT^[AC][GT]AC AccIII 329 TCCGGA T^CCGGA Alw44I 810 GTGCAC G^TGCAC AocI 124 CCTCAGG CC^T[ACGT]AGG Aor51HI 186 AGCGCT AGC^GCT ApaI 39 GGGCCC GGGCC^C ApaLI 810 GTGCAC G^TGCAC Asp700I 507 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 315 GGACC G^G[AT]CC BalI 714 TGGCCA TGG^CCA BbsI 631 GTCTTC GAAGAC(2/6) BbvCI 588 CCTCAGC CCTCAGC(-5/-2) BfaI 608 CTAG C^TAG BglI 41 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 259 CTGGAG CTGGAG(16/14) BpuAI 631 GTCTTC GAAGAC(2/6) BsaWI 329 TCCGGA [AT]^CCGG[AT] BseAI 329 TCCGGA T^CCGGA BseRI 301 CTCCTC GAGGAG(10/8) BsiEI 964 CGGCCG CG[GA][CT]^CG BsmFI 789 GTCCC GGGAC(10/14) BsmI 234 GCATTC GAATGC(1/-1) BspEI 329 TCCGGA T^CCGGA BspMI 354 GCAGGT ACCTGC(4/8) BsrI 251 CCAGT ACTGG(1/-1) BstAPI 205 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 124 CCTCAGG CC^T[ACGT]AGG DraIII 400 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 964 CGGCCG C^GGCCG EclXI 964 CGGCCG C^GGCCG Eco47III 186 AGCGCT AGC^GCT Eco52I 964 CGGCCG C^GGCCG Eco81I 124 CCTCAGG CC^T[ACGT]AGG HaeII 186 AGCGCT [GA]GCGC^[CT] HinfI 945 GAGTC G^A[ACGT]TC KspI 43 CCGCGG CCGC^GG MaeI 608 CTAG C^TAG MaeII 629 ACGT A^CGT MluNI 714 TGGCCA TGG^CCA MroI 329 TCCGGA T^CCGGA MscI 714 TGGCCA TGG^CCA NcoI 728 CCATGG C^CATGG NspI 194 GCATGC [GA]CATG^[CT] PaeR7I 554 CTCGAG C^TCGAG PflFI 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 945 GAGTC GAGTC(4/5) PvuII 8 CAGCTG CAG^CTG SacII 43 CCGCGG CCGC^GG SexAI 401 ACCTGGT A^CC[AT]GGT SphI 194 GCATGC GCATG^C Tth111I 625 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 554 CTCGAG C^TCGAG XmnI 507 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 810 GTGCAC G[AT]GC[AT]^C 841 GAGCAC BanI 772 GGCACC G^G[CT][GA]CC 897 GGTGCC BcgI 377 GCATGGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 843 GCACGACCATCG BsaI 287 GGTCTC GGTCTC(1/5) 688 GGTCTC BsgI 265 CTGCAC GTGCAG(16/14) 87 GTGCAG BsiHKAI 810 GTGCAC G[AT]GC[AT]^C 841 GAGCAC BsmAI 288 GTCTC GTCTC(1/5) 689 GTCTC BsrDI 575 CATTGC GCAATG(2/0) 723 CATTGC BssHII 65 GCGCGC G^CGCGC 92 GCGCGC BstXI 251 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 722 CCATTGCCATGG EarI 306 CTCTTC CTCTTC(1/4) 942 GAAGAG HphI 619 GGTGA GGTGA(8/7) 291 TCACC Ksp632I 306 CTCTTC CTCTTC(1/4) 942 GAAGAG MaeIII 254 GTGAC ^GT[ACGT]AC 675 GTCAC MspA1I 8 CAGCTG C[AC]G^C[GT]G 43 CCGCGG PflMI 372 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 399 CCACCTGGTGG SmlI 554 CTCGAG C^T[CT][GA]AG 868 CTCAAG Tsp45I 254 GTGAC ^GT[GC]AC 675 GTCAC Van91I 372 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 399 CCACCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AluI AvaI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI