(Circular) (Six-base) MAPSORT of: pFN21AB5672.seq Check: 145 from: 1 to: 6313 pFN21AB5672 6313 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 3249 3634 3249 Size: 385 5928 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2423 2773 3149 4376 5760 6028 1632 Size: 465 326 350 376 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 465 376 350 326 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2836 1184 Size: 1652 4661 AlwNI CAG_nnn'CTG Cuts at: 2507 4056 5628 2507 Size: 1549 1572 3192 Fragments arranged by size: 3192 1572 1549 ApaI G_GGCC'C Cuts at: 2704 2830 2704 Size: 126 6187 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 2312 3622 3848 4000 6109 1493 Size: 819 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 819 226 152 AseI AT'TA_AT Cuts at: 160 1032 2172 4867 160 Size: 872 1140 2695 1606 Fragments arranged by size: 2695 1606 1140 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3249 3634 5089 618 Size: 325 217 627 1462 385 1455 1842 Fragments arranged by size: 1842 1462 1455 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2225 2449 2704 2830 3112 3632 1391 Size: 633 201 224 255 126 282 520 4072 Fragments arranged by size: 4072 633 520 282 255 224 201 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2192 3525 928 Size: 971 293 1333 3716 Fragments arranged by size: 3716 1333 971 293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3298 3332 4504 4538 1903 Size: 34 1361 34 1172 34 3678 Fragments arranged by size: 3678 1361 1172 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 2244 1548 Size: 696 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4924 136 Size: 107 122 71 631 605 3252 1525 Fragments arranged by size: 3252 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3471 6308 3471 Size: 2837 3476 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 2575 2704 2830 4374 5530 1790 Size: 785 129 126 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 785 129 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2241 2585 2982 Size: 416 53 83 399 24 824 344 397 Cuts at: 2982 3272 4044 5002 442 Size: 290 772 958 1753 Fragments arranged by size: 1753 958 824 772 416 399 397 344 290 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2610 3359 3434 4973 1239 Size: 1371 749 75 1539 2579 Fragments arranged by size: 2579 1539 1371 749 75 Bpu10I CC'TnA_GC Cuts at: 3237 3385 3237 Size: 148 6165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4438 5303 5601 4438 Size: 865 298 5150 Fragments arranged by size: 5150 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2758 3262 4976 882 Size: 617 504 755 504 1714 2219 Fragments arranged by size: 2219 1714 755 617 504 504 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 3158 6307 3158 Size: 3149 3164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4120 4502 5137 275 Size: 53 83 186 936 2587 382 635 1451 Fragments arranged by size: 2587 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2316 2882 3060 4746 5144 5418 5565 5976 Size: 198 566 178 1686 398 274 147 411 Cuts at: 5976 2118 Size: 2455 Fragments arranged by size: 2455 1686 566 411 398 274 198 178 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6088 Fragments arranged by size: 6088 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6313 BseYI C'CCAG_C Cuts at: 2823 5516 6012 2823 Size: 2693 496 3124 Fragments arranged by size: 3124 2693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5740 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2225 3504 4374 4459 5530 2225 Size: 1279 870 85 1071 3008 Fragments arranged by size: 3008 1279 1071 870 85 BsmI GAATG_Cn' Cuts at: 2109 2695 3824 3917 2109 Size: 586 1129 93 4505 Fragments arranged by size: 4505 1129 586 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 6313 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2225 2449 2575 2704 2830 3112 Size: 399 234 201 224 126 129 126 282 Cuts at: 3112 3504 3632 4374 4459 5530 1391 Size: 392 128 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 282 234 224 201 129 128 126 126 85 BspEI T'CCGG_A Cuts at: 2118 2316 2882 3060 5976 2118 Size: 198 566 178 2916 2455 Fragments arranged by size: 2916 2455 566 198 178 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 3668 844 Size: 1454 1370 3489 Fragments arranged by size: 3489 1454 1370 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4808 4982 65 Size: 1183 3560 174 1396 Fragments arranged by size: 3560 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2420 96 Size: 2324 3989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 3458 4373 5385 3458 Size: 915 1012 4386 Fragments arranged by size: 4386 1012 915 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 3308 5117 3308 Size: 1809 4504 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstYI r'GATC_y Cuts at: 1972 2862 3471 3643 4010 4395 4412 5864 5950 Size: 890 609 172 367 385 17 1452 86 Cuts at: 5950 5962 6308 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 890 609 385 367 346 172 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3585 3585 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2955 3594 513 Size: 552 939 9 942 639 3232 Fragments arranged by size: 3232 942 939 639 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3385 3829 4623 4643 3385 Size: 444 794 20 5055 Fragments arranged by size: 5055 794 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2361 2396 3361 3396 529 Size: 35 1797 35 965 35 3446 Fragments arranged by size: 3446 1797 965 35 35 35 DraI TTT'AAA Cuts at: 2149 3617 2149 Size: 1468 4845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2885 5320 809 Size: 507 1569 2435 1802 Fragments arranged by size: 2435 1802 1569 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3760 4649 6278 Size: 54 1425 50 275 18 1930 889 1629 Cuts at: 6278 8 Size: 43 Fragments arranged by size: 1930 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2089 2097 2399 2792 4243 1353 Size: 689 47 8 302 393 1451 3423 Fragments arranged by size: 3423 1451 689 393 302 47 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2223 2223 Size: 6313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2423 2610 2773 3149 3359 3434 Size: 393 465 326 187 163 376 210 75 Cuts at: 3434 4376 4973 5760 6028 1239 Size: 942 597 787 268 1524 Fragments arranged by size: 1524 942 787 597 465 393 376 326 268 210 187 163 75 EcoNI CCTnn'n_nnAGG Cuts at: 3413 3413 Size: 6313 EcoO109I rG'GnC_Cy Cuts at: 2701 2827 3589 2701 Size: 126 762 5425 Fragments arranged by size: 5425 762 126 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 5460 1678 Size: 3782 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2601 2633 4954 4986 5028 Size: 32 135 32 1029 32 2321 32 42 Cuts at: 5028 5060 1373 Size: 32 2626 Fragments arranged by size: 2626 2321 1029 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2649 3657 3903 669 Size: 988 281 711 1008 246 3079 Fragments arranged by size: 3079 1008 988 711 281 246 HindIII A'AGCT_T Cuts at: 748 3069 748 Size: 2321 3992 HpaI GTT'AAC Cuts at: 2649 3903 2649 Size: 1254 5059 KpnI G_GTAC'C Cuts at: 3253 3638 3253 Size: 385 5928 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4491 Fragments arranged by size: 4491 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4271 4630 4789 518 Size: 648 3105 359 159 2042 Fragments arranged by size: 3105 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3729 4406 5554 5799 1606 Size: 2123 677 1148 245 2120 Fragments arranged by size: 2123 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2013 3594 513 Size: 552 939 9 1581 3232 Fragments arranged by size: 3232 1581 939 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3671 5216 1132 Size: 97 2442 1545 2229 Fragments arranged by size: 2442 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2024 1348 Size: 676 5637 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2993 1613 Size: 1380 4933 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 2700 2826 2700 Size: 126 6187 PstI C_TGCA'G Cuts at: 830 2295 3665 830 Size: 1465 1370 3478 Fragments arranged by size: 3478 1465 1370 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 SacI G_AGCT'C Cuts at: 2225 2225 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SapI GCTCTTCn'nnn_ Cuts at: 2042 2042 Size: 6313 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 6313 SfcI C'TryA_G Cuts at: 826 1045 2291 3661 4796 5477 5668 826 Size: 219 1246 1370 1135 681 191 1471 Fragments arranged by size: 1471 1370 1246 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4417 5318 5580 820 Size: 197 926 2474 901 262 1553 Fragments arranged by size: 2474 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 2458 3091 4237 5 Size: 47 2406 633 1146 2081 Fragments arranged by size: 2406 2081 1146 633 47 StuI AGG'CCT Cuts at: 1517 3485 1517 Size: 1968 4345 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 3048 3594 3752 513 Size: 552 939 9 1035 546 158 3074 Fragments arranged by size: 3074 1035 939 552 546 158 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4340 4357 4510 4695 5140 325 Size: 1255 2760 17 153 185 445 1498 Fragments arranged by size: 2760 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2420 2770 4559 Size: 274 80 33 51 1443 443 350 1789 Cuts at: 4559 6271 96 Size: 1712 138 Fragments arranged by size: 1789 1712 1443 443 350 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 6273 6305 2298 Size: 32 3943 32 2306 Fragments arranged by size: 3943 2306 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 3162 3485 4442 3162 Size: 323 957 5033 Fragments arranged by size: 5033 957 323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI