Restriction map for
pFN21AE3649
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccIII AcsI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspEI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspI SwaI
TfiI Tsp509I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 723 GGTACC G^GTACC
AclI 794 AACGTT AA^CGTT
AflII 1276 CTTAAG C^TTAAG
Asp700I 228 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 723 GGTACC G^GTACC
AspHI 85 GAGCAC G[AT]GC[AT]^C
AspI 428 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 82 CCCGAG C^[CT]CG[GA]G
BamHI 317 GGATCC G^GATCC
BbsI 172 GTCTTC GAAGAC(2/6)
BciVI 438 GTATCC GTATCC(6/5)
BcnI 430 CCCGG CC^[GC]GG
BfaI 340 CTAG C^TAG
BfrI 1276 CTTAAG C^TTAAG
BglI 1097 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 172 GTCTTC GAAGAC(2/6)
BsiHKAI 85 GAGCAC G[AT]GC[AT]^C
BsmAI 1285 GAGAC GTCTC(1/5)
BsmFI 1258 GTCCC GGGAC(10/14)
BsoBI 82 CCCGAG C^[CT]CG[GA]G
BspMI 1087 ACCTGC ACCTGC(4/8)
BsrFI 836 GCCGGC [GA]^CCGG[CT]
BstEII 955 GGTGACC G^GT[ACGT]ACC
BstPI 955 GGTGACC G^GT[ACGT]ACC
Cfr10I 836 GCCGGC [GA]^CCGG[CT]
DraIII 1192 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 767 TGGCCG [CT]^GGCC[GA]
EarI 810 GAAGAG CTCTTC(1/4)
EcoNI 660 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 955 GGTGACC G^GT[ACGT]ACC
HindIII 526 AAGCTT A^AGCTT
HinfI 112 GACTC G^A[ACGT]TC
KpnI 723 GGTACC GGTAC^C
Ksp632I 810 GAAGAG CTCTTC(1/4)
MaeI 340 CTAG C^TAG
MslI 1014 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 836 GCCGGC GCC^GGC
NciI 430 CCCGG CC^[GC]GG
NdeI 458 CATATG CA^TATG
NgoMIV 836 GCCGGC G^CCGGC
NspI 579 GCATGT [GA]CATG^[CT]
PflFI 428 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 112 GACTC GAGTC(4/5)
PpuMI 426 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 794 AACGTT AA^CGTT
SapI 810 GAAGAGC GCTCTTC(1/4)
SbfI 1002 CCTGCAGG CCTGCA^GG
Sse8387I 1002 CCTGCAGG CCTGCA^GG
TaqI 258 TCGA T^CGA
Tth111I 428 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 258 TCGA T^CGA
XmnI 228 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BanI 490 GGCACC G^G[CT][GA]CC
723 GGTACC
BanII 402 GAGCCC G[GA]GC[CT]^C
916 GGGCTC
BpmI 653 CTCCAG CTGGAG(16/14)
388 CTGGAG
BsgI 322 CTGCAC GTGCAG(16/14)
943 CTGCAC
Bsp1407I 68 TGTACA T^GTACA
1199 TGTACA
BsrBI 97 GAGCGG CCGCTC(-3/-3)
1101 GAGCGG
BsrGI 68 TGTACA T^GTACA
1199 TGTACA
BstYI 161 AGATCC [GA]^GATC[CT]
317 GGATCC
DraII 426 AGGACCC [GA]G^G[ACGT]CC[CT]
998 AGGCCCT
DsaI 301 CCATGG C^C[GA][CT]GG
741 CCATGG
EcoO109I 426 AGGACCC [GA]G^G[ACGT]CC[CT]
998 AGGCCCT
EcoT22I 473 ATGCAT ATGCA^T
577 ATGCAT
HapII 431 CCGG C^CGG
837 CCGG
HpaII 431 CCGG C^CGG
837 CCGG
MaeII 130 ACGT A^CGT
795 ACGT
MflI 161 AGATCC [GA]^GATC[CT]
317 GGATCC
MseI 515 TTAA T^TAA
1277 TTAA
MspI 431 CCGG C^CGG
837 CCGG
NcoI 301 CCATGG C^CATGG
741 CCATGG
NsiI 473 ATGCAT ATGCA^T
577 ATGCAT
PflMI 357 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
406 CCAGAAGGTGG
PstI 1003 CTGCAG CTGCA^G
1180 CTGCAG
SmlI 784 CTCAAG C^T[CT][GA]AG
1276 CTTAAG
SspBI 68 TGTACA T^GTACA
1199 TGTACA
StuI 252 AGGCCT AGG^CCT
500 AGGCCT
Tru9I 515 TTAA T^TAA
1277 TTAA
Van91I 357 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
406 CCAGAAGGTGG
XhoII 161 AGATCC [GA]^GATC[CT]
317 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI