(Circular) (Six-base) MAPSORT of: pFN21AE3649.seq Check: 6825 from: 1 to: 6016 pFN21AE3649 6016 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3101 3101 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 2728 3337 2728 Size: 609 5407 AclI AA'CG_TT Cuts at: 1563 2800 4143 4516 1563 Size: 1237 1343 373 3063 Fragments arranged by size: 3063 1343 1237 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2522 2576 4079 5463 5731 1632 Size: 532 358 54 1503 1384 268 1917 Fragments arranged by size: 1917 1503 1384 532 358 268 54 AflII C'TTAA_G Cuts at: 820 1017 3281 820 Size: 197 2264 3555 Fragments arranged by size: 3555 2264 197 AflIII A'CryG_T Cuts at: 1203 4915 5892 1203 Size: 3712 977 1327 Fragments arranged by size: 3712 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2392 3007 3305 3759 5331 2392 Size: 615 298 454 1572 3077 Fragments arranged by size: 3077 1572 615 454 298 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 3325 3551 3703 5812 1493 Size: 1832 226 152 2109 1697 Fragments arranged by size: 2109 1832 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2087 3341 1870 Size: 73 144 1254 4545 Fragments arranged by size: 4545 1254 144 73 BamHI G'GATC_C Cuts at: 2322 3346 3713 2322 Size: 1024 367 4625 Fragments arranged by size: 4625 1024 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 2728 3337 4792 618 Size: 325 217 627 708 233 609 1455 1842 Fragments arranged by size: 1842 1455 708 627 609 325 233 217 BanII G_rGCy'C Cuts at: 1391 2411 2925 3335 1391 Size: 1020 514 410 4072 Fragments arranged by size: 4072 1020 514 410 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2170 928 Size: 971 271 4774 Fragments arranged by size: 4774 971 271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2454 3908 5118 2454 Size: 1454 1210 3352 Fragments arranged by size: 3352 1454 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BfrBI ATG'CAT Cuts at: 2480 2584 2480 Size: 104 5912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3108 4627 136 Size: 107 122 71 631 605 1436 1519 1525 Fragments arranged by size: 1525 1519 1436 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2678 3041 3174 4077 5233 1790 Size: 888 363 133 903 1156 2573 Fragments arranged by size: 2573 1156 903 888 363 133 BmgBI CAC'GTC Cuts at: 1318 2136 1318 Size: 818 5198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2716 3194 3747 Size: 416 53 83 399 24 1299 478 553 Cuts at: 3747 4705 442 Size: 958 1753 Fragments arranged by size: 1753 1299 958 553 478 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2643 4676 1239 Size: 1175 229 2033 2579 Fragments arranged by size: 2579 2033 1175 229 Bpu10I CC'TnA_GC Cuts at: 2972 3104 2972 Size: 132 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 4141 5006 5304 2774 Size: 1367 865 298 3486 Fragments arranged by size: 3486 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4679 882 Size: 617 3180 2219 Fragments arranged by size: 3180 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 1178 Size: 30 165 30 1180 30 4581 Fragments arranged by size: 4581 1180 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2442 2516 3290 1186 Size: 1256 74 774 3912 Fragments arranged by size: 3912 1256 774 74 BseYI C'CCAG_C Cuts at: 3123 5219 5715 3123 Size: 2096 496 3424 Fragments arranged by size: 3424 2096 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 2933 1163 Size: 573 576 621 4246 Fragments arranged by size: 4246 621 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 4077 4162 5233 2094 Size: 1983 85 1071 2877 Fragments arranged by size: 2877 1983 1071 85 BsmI GAATG_Cn' Cuts at: 3527 3620 3527 Size: 93 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2411 2678 2925 3041 3174 3335 Size: 399 304 317 267 247 116 133 161 Cuts at: 3335 4077 4162 5233 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 317 304 267 247 161 133 116 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 3371 844 Size: 2257 270 3489 Fragments arranged by size: 3489 2257 270 BsrBI CCG'CTC Cuts at: 1394 1823 2104 3108 3903 1394 Size: 429 281 1004 795 3507 Fragments arranged by size: 3507 1004 795 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2841 4660 4847 1700 Size: 110 1031 1819 187 2869 Fragments arranged by size: 2869 1819 1031 187 110 BsrGI T'GTAC_A Cuts at: 96 2073 3204 96 Size: 1977 1131 2908 Fragments arranged by size: 2908 1977 1131 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 2621 3007 3305 794 Size: 1683 144 386 298 3505 Fragments arranged by size: 3505 1683 386 298 144 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 2960 1184 Size: 1776 4240 BstYI r'GATC_y Cuts at: 1972 2166 2322 3346 3713 4098 4115 5567 5653 Size: 194 156 1024 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1024 385 367 346 194 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2306 2746 513 Size: 552 939 302 440 3783 Fragments arranged by size: 3783 939 552 440 302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2676 2945 3532 4326 4346 2676 Size: 269 587 794 20 4346 Fragments arranged by size: 4346 794 587 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DraIII CAC_nnn'GTG Cuts at: 3202 3202 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2772 3463 4352 Size: 54 1425 50 275 18 942 691 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 942 889 691 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2810 3946 1353 Size: 1457 1136 3423 Fragments arranged by size: 3423 1457 1136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2414 2522 2576 2643 4079 4676 Size: 393 532 250 108 54 67 1436 597 Cuts at: 4676 5463 5731 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1436 787 597 532 393 268 250 108 67 54 EcoNI CCTnn'n_nnAGG Cuts at: 2669 2669 Size: 6016 EcoO109I rG'GnC_Cy Cuts at: 2432 3004 2432 Size: 572 5444 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 5163 1678 Size: 3485 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2980 3012 3278 3310 4657 Size: 32 135 32 1408 32 266 32 1347 Cuts at: 4657 4689 4731 4763 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1408 1347 266 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 2531 748 Size: 1783 4233 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 2732 3341 2732 Size: 609 5407 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 5130 5314 1305 Size: 1264 2561 184 2007 Fragments arranged by size: 2561 2007 1264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4194 Fragments arranged by size: 4194 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3023 3974 4333 4492 518 Size: 648 1857 951 359 159 2042 Fragments arranged by size: 2042 1857 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2877 3138 3432 4109 5257 5502 1606 Size: 465 806 261 294 677 1148 245 2120 Fragments arranged by size: 2120 1148 806 677 465 294 261 245 NaeI GCC'GGC Cuts at: 2843 2843 Size: 6016 NcoI C'CATG_G Cuts at: 513 1065 2004 2306 2746 513 Size: 552 939 302 440 3783 Fragments arranged by size: 3783 939 552 440 302 NdeI CA'TA_TG Cuts at: 387 2464 387 Size: 2077 3939 NgoMIV G'CCGG_C Cuts at: 2841 2841 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NsiI A_TGCA'T Cuts at: 2482 2586 2482 Size: 104 5912 NspI r_CATG'y Cuts at: 1132 1229 2588 3374 4919 1132 Size: 97 1359 786 1545 2229 Fragments arranged by size: 2229 1545 1359 786 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 2368 2417 1108 Size: 1260 49 4707 Fragments arranged by size: 4707 1260 49 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2785 2817 4085 4117 5598 5630 2785 Size: 32 1268 32 1481 32 3171 Fragments arranged by size: 3171 1481 1268 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2432 2432 Size: 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PstI C_TGCA'G Cuts at: 830 3012 3189 3368 830 Size: 2182 177 179 3478 Fragments arranged by size: 3478 2182 179 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2810 2810 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3012 3368 3012 Size: 356 5660 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2373 3008 3185 3251 3364 4499 5180 Size: 219 1328 635 177 66 113 1135 681 Cuts at: 5180 5371 826 Size: 191 1471 Fragments arranged by size: 1471 1328 1135 681 635 219 191 177 113 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2789 3281 4120 5021 5283 820 Size: 197 926 846 492 839 901 262 1553 Fragments arranged by size: 1553 926 901 846 839 492 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2259 2507 1517 Size: 742 248 5026 Fragments arranged by size: 5026 742 248 StyI C'CwwG_G Cuts at: 513 1065 2004 2210 2306 2746 2956 3455 513 Size: 552 939 206 96 440 210 499 3074 Fragments arranged by size: 3074 939 552 499 440 210 206 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2473 2821 3155 3213 4043 4060 4213 Size: 1255 893 348 334 58 830 17 153 Cuts at: 4213 4398 4843 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 893 830 445 348 334 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 2547 3015 Size: 274 80 33 51 1443 96 474 468 Cuts at: 3015 3204 4262 5974 96 Size: 189 1058 1712 138 Fragments arranged by size: 1712 1443 1058 474 468 274 189 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 5976 6008 2583 Size: 32 3361 32 2591 Fragments arranged by size: 3361 2591 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2436 1316 Size: 1120 4896 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 2237 4145 2237 Size: 1908 4108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI