(Linear) (Six-base) MAPSORT of: KIEE3649.seq Check: 9889 from: 1 to: 1311 KIEE3649.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1096 1311 Size: 1096 215 Acc65I G'GTAC_C Cuts at: 0 723 1311 Size: 723 588 AclI AA'CG_TT Cuts at: 0 795 1311 Size: 795 516 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 517 571 1311 Size: 159 358 54 740 Fragments arranged by size: 740 358 159 54 AflII C'TTAA_G Cuts at: 0 1276 1311 Size: 1276 35 AlwNI CAG_nnn'CTG Cuts at: 0 387 1002 1300 1311 Size: 387 615 298 11 Fragments arranged by size: 615 387 298 11 AvaI C'yCGr_G Cuts at: 0 82 1311 Size: 82 1229 BamHI G'GATC_C Cuts at: 0 317 1311 Size: 317 994 BanI G'GyrC_C Cuts at: 0 490 723 1311 Size: 490 233 588 Fragments arranged by size: 588 490 233 BanII G_rGCy'C Cuts at: 0 406 920 1311 Size: 406 514 391 Fragments arranged by size: 514 406 391 BbsI GAAGACnn'nnnn_ Cuts at: 0 165 1311 Size: 165 1146 BciVI GTATCCnnnnn_n' Cuts at: 0 449 1311 Size: 449 862 BfrBI ATG'CAT Cuts at: 0 475 579 1311 Size: 475 104 732 Fragments arranged by size: 732 475 104 BglI GCCn_nnn'nGGC Cuts at: 0 1103 1311 Size: 1103 208 Bme1580I G_kGCm'C Cuts at: 0 673 1036 1169 1311 Size: 673 363 133 142 Fragments arranged by size: 673 363 142 133 BmgBI CAC'GTC Cuts at: 0 131 1311 Size: 131 1180 BmrI ACTGGGnnnn_n' Cuts at: 0 711 1189 1311 Size: 711 478 122 Fragments arranged by size: 711 478 122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 638 1311 Size: 409 229 673 Fragments arranged by size: 673 409 229 Bpu10I CC'TnA_GC Cuts at: 0 967 1099 1311 Size: 967 132 212 Fragments arranged by size: 967 212 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 1311 Size: 769 542 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 578 608 1311 Size: 578 30 703 Fragments arranged by size: 703 578 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 437 511 1285 1311 Size: 437 74 774 26 Fragments arranged by size: 774 437 74 26 BseYI C'CCAG_C Cuts at: 0 1118 1311 Size: 1118 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 928 1311 Size: 307 621 383 Fragments arranged by size: 621 383 307 BsiHKAI G_wGCw'C Cuts at: 0 89 1311 Size: 89 1222 Bsp1286I G_dGCh'C Cuts at: 0 89 406 673 920 1036 1169 1311 Size: 89 317 267 247 116 133 142 Fragments arranged by size: 317 267 247 142 133 116 89 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1096 1311 Size: 1096 215 BsrBI CCG'CTC Cuts at: 0 99 1103 1311 Size: 99 1004 208 Fragments arranged by size: 1004 208 99 BsrFI r'CCGG_y Cuts at: 0 836 1311 Size: 836 475 BsrGI T'GTAC_A Cuts at: 0 68 1199 1311 Size: 68 1131 112 Fragments arranged by size: 1131 112 68 BstAPI GCAn_nnn'nTGC Cuts at: 0 472 616 1002 1300 1311 Size: 472 144 386 298 11 Fragments arranged by size: 472 386 298 144 11 BstEII G'GTnAC_C Cuts at: 0 955 1311 Size: 955 356 BstYI r'GATC_y Cuts at: 0 161 317 1311 Size: 161 156 994 Fragments arranged by size: 994 161 156 BtgI C'CryG_G Cuts at: 0 301 741 1311 Size: 301 440 570 Fragments arranged by size: 570 440 301 BtsI GCAGTG_nn' Cuts at: 0 671 940 1311 Size: 671 269 371 Fragments arranged by size: 671 371 269 DraIII CAC_nnn'GTG Cuts at: 0 1197 1311 Size: 1197 114 EaeI y'GGCC_r Cuts at: 0 767 1311 Size: 767 544 EarI CTCTTCn'nnn_ Cuts at: 0 805 1311 Size: 805 506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 409 517 571 638 1311 Size: 159 250 108 54 67 673 Fragments arranged by size: 673 250 159 108 67 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 664 1311 Size: 664 647 EcoO109I rG'GnC_Cy Cuts at: 0 427 999 1311 Size: 427 572 312 Fragments arranged by size: 572 427 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 975 1007 1273 1305 1311 Size: 975 32 266 32 6 Fragments arranged by size: 975 266 32 32 6 HindIII A'AGCT_T Cuts at: 0 526 1311 Size: 526 785 KpnI G_GTAC'C Cuts at: 0 727 1311 Size: 727 584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 564 1311 Size: 564 747 MslI CAynn'nnrTG Cuts at: 0 1018 1311 Size: 1018 293 MspA1I CmG'CkG Cuts at: 0 66 872 1133 1311 Size: 66 806 261 178 Fragments arranged by size: 806 261 178 66 NaeI GCC'GGC Cuts at: 0 838 1311 Size: 838 473 NcoI C'CATG_G Cuts at: 0 301 741 1311 Size: 301 440 570 Fragments arranged by size: 570 440 301 NdeI CA'TA_TG Cuts at: 0 459 1311 Size: 459 852 NgoMIV G'CCGG_C Cuts at: 0 836 1311 Size: 836 475 NsiI A_TGCA'T Cuts at: 0 477 581 1311 Size: 477 104 730 Fragments arranged by size: 730 477 104 NspI r_CATG'y Cuts at: 0 583 1311 Size: 583 728 PflMI CCAn_nnn'nTGG Cuts at: 0 363 412 1311 Size: 363 49 899 Fragments arranged by size: 899 363 49 PfoI T'CCnGG_A Cuts at: 0 386 1311 Size: 386 925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 780 812 1311 Size: 780 32 499 Fragments arranged by size: 780 499 32 PpuMI rG'GwC_Cy Cuts at: 0 427 1311 Size: 427 884 PstI C_TGCA'G Cuts at: 0 1007 1184 1311 Size: 1007 177 127 Fragments arranged by size: 1007 177 127 SapI GCTCTTCn'nnn_ Cuts at: 0 805 1311 Size: 805 506 SbfI CC_TGCA'GG Cuts at: 0 1007 1311 Size: 1007 304 SfcI C'TryA_G Cuts at: 0 368 1003 1180 1246 1311 Size: 368 635 177 66 65 Fragments arranged by size: 635 368 177 66 65 SmlI C'TyrA_G Cuts at: 0 784 1276 1311 Size: 784 492 35 Fragments arranged by size: 784 492 35 StuI AGG'CCT Cuts at: 0 254 502 1311 Size: 254 248 809 Fragments arranged by size: 809 254 248 StyI C'CwwG_G Cuts at: 0 205 301 741 951 1311 Size: 205 96 440 210 360 Fragments arranged by size: 440 360 210 205 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 468 816 1150 1208 1311 Size: 468 348 334 58 103 Fragments arranged by size: 468 348 334 103 58 TatI w'GTAC_w Cuts at: 0 68 542 1010 1199 1311 Size: 68 474 468 189 112 Fragments arranged by size: 474 468 189 112 68 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 578 610 1311 Size: 578 32 701 Fragments arranged by size: 701 578 32 Tth111I GACn'n_nGTC Cuts at: 0 431 1311 Size: 431 880 XmnI GAAnn'nnTTC Cuts at: 0 232 1311 Size: 232 1079 Enzymes that do cut: AarI Acc65I AclI AcuI AflII AlwNI AvaI BamHI BanI BanII BbsI BciVI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HindIII KpnI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PflMI PfoI PpiI PpuMI PstI SapI SbfI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AatII AccI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BglII BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI MfeI MluI MscI NarI NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI