(Circular) (Six-base) MAPSORT of: pFN21AE4557.seq Check: 3960 from: 1 to: 6742 pFN21AE4557 6742 bp With 182 enzymes: SgfI * March 28, 2012 14:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4552 278 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2648 4085 1937 Size: 711 1437 4594 Fragments arranged by size: 4594 1437 711 Acc65I G'GTAC_C Cuts at: 2693 4063 2693 Size: 1370 5372 AclI AA'CG_TT Cuts at: 1563 4869 5242 1563 Size: 3306 373 3063 Fragments arranged by size: 3306 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 3552 3787 4003 4805 6189 6457 1632 Size: 569 1351 235 216 802 1384 268 1917 Fragments arranged by size: 1917 1384 1351 802 569 268 235 216 AflII C'TTAA_G Cuts at: 820 1017 3238 820 Size: 197 2221 4324 Fragments arranged by size: 4324 2221 197 AflIII A'CryG_T Cuts at: 1203 5641 6618 1203 Size: 4438 977 1327 Fragments arranged by size: 4438 1327 977 AgeI A'CCGG_T Cuts at: 5573 5573 Size: 6742 AhdI GACnn_n'nnGTC Cuts at: 5471 5471 Size: 6742 AleI CACnn'nnGTG Cuts at: 1166 3833 1166 Size: 2667 4075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6742 AlwNI CAG_nnn'CTG Cuts at: 3405 3437 3556 4485 6057 3405 Size: 32 119 929 1572 4090 Fragments arranged by size: 4090 1572 929 119 32 ApaLI G'TGCA_C Cuts at: 4799 5955 4799 Size: 1156 5586 ApoI r'AATT_y Cuts at: 1493 2140 2526 3378 4051 4277 4429 6538 1493 Size: 647 386 852 673 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 673 647 386 226 152 AseI AT'TA_AT Cuts at: 160 1032 5296 160 Size: 872 4264 1606 Fragments arranged by size: 4264 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 AvaI C'yCGr_G Cuts at: 1870 1943 4067 1870 Size: 73 2124 4545 Fragments arranged by size: 4545 2124 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2142 2175 2142 Size: 33 6709 BamHI G'GATC_C Cuts at: 4072 4439 4072 Size: 367 6375 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2693 4063 5518 618 Size: 325 217 627 906 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 906 627 325 217 BanII G_rGCy'C Cuts at: 1391 2165 2225 3890 4061 1391 Size: 774 60 1665 171 4072 Fragments arranged by size: 4072 1665 774 171 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 928 Size: 971 951 4820 Fragments arranged by size: 4820 971 951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4933 4967 1903 Size: 34 2996 34 3678 Fragments arranged by size: 3678 2996 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4634 5844 4634 Size: 1210 5532 BclI T'GATC_A Cuts at: 1548 1548 Size: 6742 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4010 5353 136 Size: 107 122 71 631 605 2338 1343 1525 Fragments arranged by size: 2338 1525 1343 631 605 122 107 71 BglII A'GATC_T Cuts at: 6737 6737 Size: 6742 BlpI GC'TnA_GC Cuts at: 4159 4159 Size: 6742 Bme1580I G_kGCm'C Cuts at: 1790 2734 2925 4803 5959 1790 Size: 944 191 1878 1156 2573 Fragments arranged by size: 2573 1878 1156 944 191 BmgBI CAC'GTC Cuts at: 1318 2278 1318 Size: 960 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2243 4473 5431 Size: 416 53 83 399 24 826 2230 958 Cuts at: 5431 442 Size: 1753 Fragments arranged by size: 2230 1753 958 826 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2205 1056 Size: 1149 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2673 2912 2970 3103 4008 5402 1239 Size: 1434 239 58 133 905 1394 2579 Fragments arranged by size: 2579 1434 1394 905 239 133 58 Bpu10I CC'TnA_GC Cuts at: 2164 2164 Size: 6742 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2283 4867 5732 6030 2283 Size: 2584 865 298 2995 Fragments arranged by size: 2995 2584 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5405 882 Size: 617 3906 2219 Fragments arranged by size: 3906 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4533 493 Size: 711 3329 2702 Fragments arranged by size: 3329 2702 711 BsaBI GATnn'nnATC Cuts at: 2078 6736 2078 Size: 4658 2084 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4549 4931 5566 Size: 53 83 186 936 2404 612 382 635 Cuts at: 5566 275 Size: 1451 Fragments arranged by size: 2404 1451 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2259 3224 3912 5175 5573 5847 5994 6405 2259 Size: 965 688 1263 398 274 147 411 2596 Fragments arranged by size: 2596 1263 965 688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6517 Fragments arranged by size: 6517 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2957 3533 1186 Size: 1771 576 4395 Fragments arranged by size: 4395 1771 576 BseYI C'CCAG_C Cuts at: 2665 3005 5945 6441 2665 Size: 340 2940 496 2966 Fragments arranged by size: 2966 2940 496 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2599 2739 3075 3080 1163 Size: 573 863 140 336 5 4825 Fragments arranged by size: 4825 863 573 336 140 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4192 4953 5981 1540 Size: 275 186 2191 761 1028 2301 Fragments arranged by size: 2301 2191 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2659 4803 4888 5959 2659 Size: 2144 85 1071 3442 Fragments arranged by size: 3442 2144 1071 85 BsmI GAATG_Cn' Cuts at: 2111 2549 4253 4346 2111 Size: 438 1704 93 4507 Fragments arranged by size: 4507 1704 438 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3944 3944 Size: 6742 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2225 2659 2734 2925 3890 4061 Size: 399 375 60 434 75 191 965 171 Cuts at: 4061 4803 4888 5959 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 965 742 434 399 375 191 171 85 75 60 BspEI T'CCGG_A Cuts at: 2259 6405 2259 Size: 4146 2596 BspHI T'CATG_A Cuts at: 4631 6361 4631 Size: 1730 5012 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4097 844 Size: 3253 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4629 1394 Size: 429 2806 3507 Fragments arranged by size: 3507 2806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3415 3607 3619 5237 5411 65 Size: 1183 2167 192 12 1618 174 1396 Fragments arranged by size: 2167 1618 1396 1183 192 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2315 5386 5573 1700 Size: 110 505 3071 187 2869 Fragments arranged by size: 3071 2869 505 187 110 BsrGI T'GTAC_A Cuts at: 96 2286 3165 96 Size: 2190 879 3673 Fragments arranged by size: 3673 2190 879 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6742 BssSI C'ACGA_G Cuts at: 2357 2429 4802 5814 2357 Size: 72 2373 1012 3285 Fragments arranged by size: 3285 2373 1012 72 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6742 BstBI TT'CG_AA Cuts at: 3382 5546 3382 Size: 2164 4578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6742 BstYI r'GATC_y Cuts at: 1972 2569 3561 3772 4072 4439 4824 4841 6293 Size: 597 992 211 300 367 385 17 1452 Cuts at: 6293 6379 6391 6737 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 992 597 385 367 346 300 211 86 17 12 BstZ17I GTA'TAC Cuts at: 2649 2649 Size: 6742 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 3270 3592 3762 513 Size: 552 939 114 1152 322 170 3493 Fragments arranged by size: 3493 1152 939 552 322 170 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6003 Fragments arranged by size: 6003 683 56 BtsI GCAGTG_nn' Cuts at: 2501 3063 4258 5052 5072 2501 Size: 562 1195 794 20 4171 Fragments arranged by size: 4171 1195 794 562 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6707 DraI TTT'AAA Cuts at: 4046 4046 Size: 6742 DraIII CAC_nnn'GTG Cuts at: 3145 3145 Size: 6742 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3452 5749 809 Size: 507 2136 2297 1802 Fragments arranged by size: 2297 2136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 3124 4189 Size: 54 1425 50 275 18 483 811 1065 Cuts at: 4189 5078 6707 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1065 889 811 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4189 1537 Size: 275 2377 4090 Fragments arranged by size: 4090 2377 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3551 3742 4672 1353 Size: 1409 789 191 930 3423 Fragments arranged by size: 3423 1409 930 789 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5329 5703 5849 387 Size: 1064 138 3740 374 146 1280 Fragments arranged by size: 3740 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2673 2912 2970 3103 3552 3787 Size: 393 569 472 239 58 133 449 235 Cuts at: 3787 4003 4008 4805 5402 6189 6457 1239 Size: 216 5 797 597 787 268 1524 Fragments arranged by size: 1524 797 787 597 569 472 449 393 268 239 235 216 133 58 5 EcoNI CCTnn'n_nnAGG Cuts at: 2561 2561 Size: 6742 EcoO109I rG'GnC_Cy Cuts at: 2909 2909 Size: 6742 EcoRI G'AATT_C Cuts at: 3378 4051 4429 3378 Size: 673 378 5691 Fragments arranged by size: 5691 673 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 6742 FspI TGC'GCA Cuts at: 5248 5248 Size: 6742 HaeII r_GCGC'y Cuts at: 1678 5889 1678 Size: 4211 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3413 3445 4014 4046 5383 Size: 32 135 32 1841 32 569 32 1337 Cuts at: 5383 5415 5457 5489 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1841 1337 569 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3931 4086 4332 669 Size: 988 281 1993 155 246 3079 Fragments arranged by size: 3079 1993 988 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 6742 HpaI GTT'AAC Cuts at: 4332 4332 Size: 6742 KpnI G_GTAC'C Cuts at: 2697 4067 2697 Size: 1370 5372 MfeI C'AATT_G Cuts at: 4341 4341 Size: 6742 MluI A'CGCG_T Cuts at: 6618 6618 Size: 6742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2602 5856 6040 1305 Size: 1297 3254 184 2007 Fragments arranged by size: 3254 2007 1297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3126 10 Size: 54 1425 343 1294 3626 Fragments arranged by size: 3626 1425 1294 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 3688 3833 3956 4700 5059 5218 Size: 648 1542 980 145 123 744 359 159 Cuts at: 5218 518 Size: 2042 Fragments arranged by size: 2042 1542 980 744 648 359 159 145 123 MspA1I CmG'CkG Cuts at: 1606 2033 2156 4158 4835 5983 6228 1606 Size: 427 123 2002 677 1148 245 2120 Fragments arranged by size: 2120 2002 1148 677 427 245 123 NaeI GCC'GGC Cuts at: 2317 2317 Size: 6742 NcoI C'CATG_G Cuts at: 513 1065 2004 3270 3592 3762 513 Size: 552 939 1266 322 170 3493 Fragments arranged by size: 3493 1266 939 552 322 170 NdeI CA'TA_TG Cuts at: 387 387 Size: 6742 NgoMIV G'CCGG_C Cuts at: 2315 2315 Size: 6742 NheI G'CTAG_C Cuts at: 1052 2201 1052 Size: 1149 5593 NotI GC'GGCC_GC Cuts at: 4189 4189 Size: 6742 NspI r_CATG'y Cuts at: 1132 1229 3289 4100 5645 1132 Size: 97 2060 811 1545 2229 Fragments arranged by size: 2229 2060 1545 811 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6742 PciI A'CATG_T Cuts at: 5641 5641 Size: 6742 PflMI CCAn_nnn'nTGG Cuts at: 1108 2935 3763 1108 Size: 1827 828 4087 Fragments arranged by size: 4087 1827 828 PmeI GTTT'AAAC Cuts at: 4046 4046 Size: 6742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4811 4843 6324 6356 4811 Size: 32 1481 32 5197 Fragments arranged by size: 5197 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3936 1613 Size: 2323 4419 PsiI TTA'TAA Cuts at: 4312 4312 Size: 6742 PstI C_TGCA'G Cuts at: 830 3561 4094 830 Size: 2731 533 3478 Fragments arranged by size: 3478 2731 533 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6742 PvuII CAG'CTG Cuts at: 2033 2156 2033 Size: 123 6619 SalI G'TCGA_C Cuts at: 1936 4084 1936 Size: 2148 4594 SbfI CC_TGCA'GG Cuts at: 4094 4094 Size: 6742 ScaI AGT'ACT Cuts at: 2026 4990 6702 2026 Size: 2964 1712 2066 Fragments arranged by size: 2964 2066 1712 SfcI C'TryA_G Cuts at: 826 1045 2028 3557 4090 5225 5906 6097 826 Size: 219 983 1529 533 1135 681 191 1471 Fragments arranged by size: 1529 1471 1135 983 681 533 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6742 SmaI CCC'GGG Cuts at: 1872 4069 1872 Size: 2197 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2298 3238 4846 5747 6009 820 Size: 197 926 355 940 1608 901 262 1553 Fragments arranged by size: 1608 1553 940 926 901 355 262 197 SnaBI TAC'GTA Cuts at: 493 4533 493 Size: 4040 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6742 SphI G_CATG'C Cuts at: 1132 3289 4100 1132 Size: 2157 811 3774 Fragments arranged by size: 3774 2157 811 SspI AAT'ATT Cuts at: 5 52 3850 4666 5 Size: 47 3798 816 2081 Fragments arranged by size: 3798 2081 816 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6742 StyI C'CwwG_G Cuts at: 513 1065 2004 3198 3270 3592 3762 4181 513 Size: 552 939 1194 72 322 170 419 3074 Fragments arranged by size: 3074 1194 939 552 419 322 170 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4769 4786 4939 5124 5569 325 Size: 1255 3189 17 153 185 445 1498 Fragments arranged by size: 3189 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2024 2286 3165 Size: 274 80 33 51 1443 47 262 879 Cuts at: 3165 3866 4988 6700 96 Size: 701 1122 1712 138 Fragments arranged by size: 1712 1443 1122 879 701 274 262 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6702 6734 6702 Size: 32 6710 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6742 XbaI T'CTAG_A Cuts at: 3502 4078 3502 Size: 576 6166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 3625 2402 Size: 1223 5519 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6742 XmaI C'CCGG_G Cuts at: 1870 4067 1870 Size: 2197 4545 XmnI GAAnn'nnTTC Cuts at: 4871 4871 Size: 6742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4550 276 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI