(Linear) (Six-base) MAPSORT of: KIEE4557.seq Check: 7259 from: 1 to: 2037 KIEE4557.orf With 182 enzymes: SgfI * March 22, 2012 18:22 .. AccI GT'mk_AC Cuts at: 0 643 2037 Size: 643 1394 Acc65I G'GTAC_C Cuts at: 0 688 2037 Size: 688 1349 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 1547 1782 1998 2037 Size: 196 1351 235 216 39 Fragments arranged by size: 1351 235 216 196 39 AflII C'TTAA_G Cuts at: 0 1233 2037 Size: 1233 804 AleI CACnn'nnGTG Cuts at: 0 1828 2037 Size: 1828 209 AlwNI CAG_nnn'CTG Cuts at: 0 1400 1432 1551 2037 Size: 1400 32 119 486 Fragments arranged by size: 1400 486 119 32 ApoI r'AATT_y Cuts at: 0 135 521 1373 2037 Size: 135 386 852 664 Fragments arranged by size: 852 664 386 135 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 137 170 2037 Size: 137 33 1867 Fragments arranged by size: 1867 137 33 BanI G'GyrC_C Cuts at: 0 688 2037 Size: 688 1349 BanII G_rGCy'C Cuts at: 0 160 220 1885 2037 Size: 160 60 1665 152 Fragments arranged by size: 1665 160 152 60 BbsI GAAGACnn'nnnn_ Cuts at: 0 845 2037 Size: 845 1192 BglI GCCn_nnn'nGGC Cuts at: 0 2005 2037 Size: 2005 32 Bme1580I G_kGCm'C Cuts at: 0 729 920 2037 Size: 729 191 1117 Fragments arranged by size: 1117 729 191 BmgBI CAC'GTC Cuts at: 0 273 2037 Size: 273 1764 BmrI ACTGGGnnnn_n' Cuts at: 0 238 2037 Size: 238 1799 BmtI G_CTAG'C Cuts at: 0 200 2037 Size: 200 1837 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 668 907 965 1098 2003 2037 Size: 668 239 58 133 905 34 Fragments arranged by size: 905 668 239 133 58 34 Bpu10I CC'TnA_GC Cuts at: 0 159 2037 Size: 159 1878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 2037 Size: 278 1759 BsaBI GATnn'nnATC Cuts at: 0 73 2037 Size: 73 1964 BsaHI Gr'CG_yC Cuts at: 0 1932 2037 Size: 1932 105 BsaWI w'CCGG_w Cuts at: 0 254 1219 1907 2037 Size: 254 965 688 130 Fragments arranged by size: 965 688 254 130 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 952 1528 2037 Size: 952 576 509 Fragments arranged by size: 952 576 509 BseYI C'CCAG_C Cuts at: 0 660 1000 2037 Size: 660 340 1037 Fragments arranged by size: 1037 660 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 734 1070 1075 2037 Size: 594 140 336 5 962 Fragments arranged by size: 962 594 336 140 5 BsiHKAI G_wGCw'C Cuts at: 0 654 2037 Size: 654 1383 BsmI GAATG_Cn' Cuts at: 0 106 544 2037 Size: 106 438 1493 Fragments arranged by size: 1493 438 106 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1939 2037 Size: 1939 98 Bsp1286I G_dGCh'C Cuts at: 0 160 220 654 729 920 1885 2037 Size: 160 60 434 75 191 965 152 Fragments arranged by size: 965 434 191 160 152 75 60 BspEI T'CCGG_A Cuts at: 0 254 2037 Size: 254 1783 BsrDI GCAATG_nn' Cuts at: 0 1410 1602 1614 2037 Size: 1410 192 12 423 Fragments arranged by size: 1410 423 192 12 BsrFI r'CCGG_y Cuts at: 0 310 2037 Size: 310 1727 BsrGI T'GTAC_A Cuts at: 0 281 1160 2037 Size: 281 879 877 Fragments arranged by size: 879 877 281 BssSI C'ACGA_G Cuts at: 0 352 424 2037 Size: 352 72 1613 Fragments arranged by size: 1613 352 72 BstBI TT'CG_AA Cuts at: 0 1377 2037 Size: 1377 660 BstYI r'GATC_y Cuts at: 0 564 1556 1767 2037 Size: 564 992 211 270 Fragments arranged by size: 992 564 270 211 BstZ17I GTA'TAC Cuts at: 0 644 2037 Size: 644 1393 BtgI C'CryG_G Cuts at: 0 113 1265 1587 1757 2037 Size: 113 1152 322 170 280 Fragments arranged by size: 1152 322 280 170 113 BtsI GCAGTG_nn' Cuts at: 0 496 1058 2037 Size: 496 562 979 Fragments arranged by size: 979 562 496 DraIII CAC_nnn'GTG Cuts at: 0 1140 2037 Size: 1140 897 DrdI GACnn_nn'nnGTC Cuts at: 0 1447 2037 Size: 1447 590 EaeI y'GGCC_r Cuts at: 0 308 1119 2037 Size: 308 811 918 Fragments arranged by size: 918 811 308 EarI CTCTTCn'nnn_ Cuts at: 0 757 1546 1737 2037 Size: 757 789 191 300 Fragments arranged by size: 789 757 300 191 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 668 907 965 1098 1547 1782 1998 Size: 196 472 239 58 133 449 235 216 Cuts at: 1998 2003 2037 Size: 5 34 Fragments arranged by size: 472 449 239 235 216 196 133 58 34 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 556 2037 Size: 556 1481 EcoO109I rG'GnC_Cy Cuts at: 0 904 2037 Size: 904 1133 EcoRI G'AATT_C Cuts at: 0 1373 2037 Size: 1373 664 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 846 2037 Size: 846 1191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1408 1440 2009 2037 Size: 1408 32 569 28 Fragments arranged by size: 1408 569 32 28 HincII GTy'rAC Cuts at: 0 1926 2037 Size: 1926 111 KpnI G_GTAC'C Cuts at: 0 692 2037 Size: 692 1345 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 597 2037 Size: 597 1440 MscI TGG'CCA Cuts at: 0 1121 2037 Size: 1121 916 MslI CAynn'nnrTG Cuts at: 0 703 1683 1828 1951 2037 Size: 703 980 145 123 86 Fragments arranged by size: 980 703 145 123 86 MspA1I CmG'CkG Cuts at: 0 28 151 2037 Size: 28 123 1886 Fragments arranged by size: 1886 123 28 NaeI GCC'GGC Cuts at: 0 312 2037 Size: 312 1725 NcoI C'CATG_G Cuts at: 0 1265 1587 1757 2037 Size: 1265 322 170 280 Fragments arranged by size: 1265 322 280 170 NgoMIV G'CCGG_C Cuts at: 0 310 2037 Size: 310 1727 NheI G'CTAG_C Cuts at: 0 196 2037 Size: 196 1841 NspI r_CATG'y Cuts at: 0 1284 2037 Size: 1284 753 PflMI CCAn_nnn'nTGG Cuts at: 0 930 1758 2037 Size: 930 828 279 Fragments arranged by size: 930 828 279 PshAI GACnn'nnGTC Cuts at: 0 1931 2037 Size: 1931 106 PstI C_TGCA'G Cuts at: 0 1556 2037 Size: 1556 481 PvuII CAG'CTG Cuts at: 0 28 151 2037 Size: 28 123 1886 Fragments arranged by size: 1886 123 28 ScaI AGT'ACT Cuts at: 0 21 2037 Size: 21 2016 SfcI C'TryA_G Cuts at: 0 23 1552 2037 Size: 23 1529 485 Fragments arranged by size: 1529 485 23 SmlI C'TyrA_G Cuts at: 0 293 1233 2037 Size: 293 940 804 Fragments arranged by size: 940 804 293 SphI G_CATG'C Cuts at: 0 1284 2037 Size: 1284 753 SspI AAT'ATT Cuts at: 0 1845 2037 Size: 1845 192 StyI C'CwwG_G Cuts at: 0 1193 1265 1587 1757 2037 Size: 1193 72 322 170 280 Fragments arranged by size: 1193 322 280 170 72 TatI w'GTAC_w Cuts at: 0 19 281 1160 1861 2037 Size: 19 262 879 701 176 Fragments arranged by size: 879 701 262 176 19 XbaI T'CTAG_A Cuts at: 0 1497 2037 Size: 1497 540 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 397 1620 2037 Size: 397 1223 417 Fragments arranged by size: 1223 417 397 Enzymes that do cut: AccI Acc65I AcuI AflII AleI AlwNI ApoI BaeI BanI BanII BbsI BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI BsrGI BssSI BstBI BstYI BstZ17I BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PflMI PshAI PstI PvuII ScaI SfcI SmlI SphI SspI StyI TatI XbaI XcmI Enzymes that do not cut: AarI AatII AclI AfeI AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BplI BsaI BsaAI BsaXI BsiEI BsiWI BspHI BspMI BsrBI BssHII BstAPI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NarI NdeI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII TstI Tth111I XhoI XmaI XmnI ZraI