################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BciVI BfrI BglI BglII BlnI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MfeI MluI MroI MunI NdeI NotI NsiI NspI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1747 GGTACC G^GTACC AccI 2689 GTCTAC GT^[AC][GT]AC AcsI 1594 GAATTT [GA]^AATT[CT] ApaI 2215 GGGCCC GGGCC^C ApoI 1594 GAATTT [GA]^AATT[CT] Asp718I 1747 GGTACC G^GTACC BamHI 1834 GGATCC G^GATCC BbeI 982 GGCGCC GGCGC^C BbrPI 2665 CACGTG CAC^GTG BbsI 727 GTCTTC GAAGAC(2/6) BclI 1940 TGATCA T^GATCA BlpI 519 GCTGAGC GC^T[ACGT]AGC Bpu1102I 519 GCTGAGC GC^T[ACGT]AGC BpuAI 727 GTCTTC GAAGAC(2/6) BsaAI 2665 CACGTG [CT]AC^GT[GA] BsaBI 957 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 267 CGACCG CG[GA][CT]^CG BsmBI 1289 GAGACG CGTCTC(1/5) BsmI 1464 GAATGC GAATGC(1/-1) Bsp1407I 1070 TGTACA T^GTACA BsrGI 1070 TGTACA T^GTACA BstAPI 2661 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1158 GGTGACC G^GT[ACGT]ACC BstPI 1158 GGTGACC G^GT[ACGT]ACC CelII 519 GCTGAGC GC^T[ACGT]AGC DraIII 1762 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1708 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1158 GGTGACC G^GT[ACGT]ACC FbaI 1940 TGATCA T^GATCA KasI 982 GGCGCC G^GCGCC KpnI 1747 GGTACC GGTAC^C MamI 957 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1603 TTAA T^TAA NarI 982 GGCGCC GG^CGCC NcoI 2621 CCATGG C^CATGG NheI 214 GCTAGC G^CTAGC NruI 152 TCGCGA TCG^CGA PflMI 2752 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2665 CACGTG CAC^GTG PmlI 2665 CACGTG CAC^GTG PstI 2659 CTGCAG CTGCA^G ScaI 1885 AGTACT AGT^ACT SexAI 2325 ACCTGGT A^CC[AT]GGT SfoI 982 GGCGCC GGC^GCC SgrAI 864 CGCCGGCG C[GA]^CCGG[CT]G SspBI 1070 TGTACA T^GTACA Tru9I 1603 TTAA T^TAA Van91I 2752 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2167 TCTAGA T^CTAGA XcmI 1232 CCAGTGCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 1758 TGGCCA TGG^CCA 2345 TGGCCA BbvCI 1272 CCTCAGC CCTCAGC(-5/-2) 1751 CCTCAGC BcgI 143 GCAACGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1461 GCAGAATGCTCG BsaWI 247 ACCGGA [AT]^CCGG[AT] 2379 TCCGGT BseRI 18 GAGGAG GAGGAG(10/8) 283 GAGGAG BsrBI 463 CCGCTC CCGCTC(-3/-3) 21 GAGCGG EcoT14I 159 CCTTGG C^C[AT][AT]GG 2621 CCATGG MluNI 1758 TGGCCA TGG^CCA 2345 TGGCCA MscI 1758 TGGCCA TGG^CCA 2345 TGGCCA NaeI 865 GCCGGC GCC^GGC 2614 GCCGGC NgoMIV 865 GCCGGC G^CCGGC 2614 GCCGGC PaeR7I 466 CTCGAG C^TCGAG 1742 CTCGAG PvuII 1754 CAGCTG CAG^CTG 2726 CAGCTG SmaI 1414 CCCGGG CCC^GGG 2048 CCCGGG StuI 11 AGGCCT AGG^CCT 2247 AGGCCT StyI 159 CCTTGG C^C[AT][AT]GG 2621 CCATGG TfiI 224 GATTC G^A[AT]TC 1912 GATTC XhoI 466 CTCGAG C^TCGAG 1742 CTCGAG XmaCI 1414 CCCGGG C^CCGGG 2048 CCCGGG XmaI 1414 CCCGGG C^CCGGG 2048 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI