(Circular) (Six-base) MAPSORT of: pFN21AE6712.seq Check: 7182 from: 1 to: 7507 pFN21AE6712 7507 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4390 4751 4390 Size: 361 7146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5317 278 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4695 4850 1937 Size: 2758 155 4594 Fragments arranged by size: 4594 2758 155 Acc65I G'GTAC_C Cuts at: 3752 4828 3752 Size: 1076 6431 AclI AA'CG_TT Cuts at: 1563 5634 6007 1563 Size: 4071 373 3063 Fragments arranged by size: 4071 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4038 4343 5570 6954 7222 1632 Size: 2406 305 1227 1384 268 1917 Fragments arranged by size: 2406 1917 1384 1227 305 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7310 AflIII A'CryG_T Cuts at: 1203 6406 7383 1203 Size: 5203 977 1327 Fragments arranged by size: 5203 1327 977 AgeI A'CCGG_T Cuts at: 6338 6338 Size: 7507 AhdI GACnn_n'nnGTC Cuts at: 6236 6236 Size: 7507 AleI CACnn'nnGTG Cuts at: 1166 2789 3071 1166 Size: 1623 282 5602 Fragments arranged by size: 5602 1623 282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7507 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2075 Size: 32 7475 AlwNI CAG_nnn'CTG Cuts at: 5250 6822 5250 Size: 1572 5935 ApaI G_GGCC'C Cuts at: 4224 4224 Size: 7507 ApaLI G'TGCA_C Cuts at: 5564 6720 5564 Size: 1156 6351 ApoI r'AATT_y Cuts at: 1493 3599 4816 5042 5194 7303 1493 Size: 2106 1217 226 152 2109 1697 Fragments arranged by size: 2109 2106 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 6061 160 Size: 872 5029 1606 Fragments arranged by size: 5029 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 AvaI C'yCGr_G Cuts at: 1870 1943 2471 3089 3291 3419 3747 4023 4053 Size: 73 528 618 202 128 328 276 30 Cuts at: 4053 4832 1870 Size: 779 4545 Fragments arranged by size: 4545 779 618 528 328 276 202 128 73 30 BamHI G'GATC_C Cuts at: 3839 4837 5204 3839 Size: 998 367 6142 Fragments arranged by size: 6142 998 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2987 3632 3752 4484 Size: 325 217 627 288 912 645 120 732 Cuts at: 4484 4517 4616 4622 4828 6283 618 Size: 33 99 6 206 1455 1842 Fragments arranged by size: 1842 1455 912 732 645 627 325 288 217 206 120 99 33 6 BanII G_rGCy'C Cuts at: 1391 3427 3731 4192 4202 4224 4601 4826 1391 Size: 2036 304 461 10 22 377 225 4072 Fragments arranged by size: 4072 2036 461 377 304 225 22 10 BbeI G_GCGC'C Cuts at: 2991 2991 Size: 7507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2725 928 Size: 971 826 5710 Fragments arranged by size: 5710 971 826 BbvCI CC'TCA_GC Cuts at: 3278 3757 3278 Size: 479 7028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2137 2171 3455 3489 5698 5732 1903 Size: 34 200 34 1284 34 2209 34 3678 Fragments arranged by size: 3678 2209 1284 200 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5399 6609 5399 Size: 1210 6297 BclI T'GATC_A Cuts at: 1548 3945 1548 Size: 2397 5110 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6118 136 Size: 107 122 71 631 605 4446 1525 Fragments arranged by size: 4446 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7502 7502 Size: 7507 BlpI GC'TnA_GC Cuts at: 2525 4924 2525 Size: 2399 5108 Bme1580I G_kGCm'C Cuts at: 1790 3576 3635 3810 4224 4677 5568 6724 1790 Size: 1786 59 175 414 453 891 1156 2573 Fragments arranged by size: 2573 1786 1156 891 453 414 175 59 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7507 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3077 4648 5238 Size: 416 53 83 399 24 1660 1571 590 Cuts at: 5238 6196 442 Size: 958 1753 Fragments arranged by size: 1753 1660 1571 958 590 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2223 1056 Size: 1167 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4054 4440 4054 Size: 386 7121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2235 2348 2372 2618 2786 6167 1239 Size: 802 194 113 24 246 168 3381 2579 Fragments arranged by size: 3381 2579 802 246 194 168 113 24 Bpu10I CC'TnA_GC Cuts at: 3278 3757 3278 Size: 479 7028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3197 5632 6497 6795 3197 Size: 2435 865 298 3909 Fragments arranged by size: 3909 2435 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 3191 4170 4471 6170 882 Size: 617 744 948 979 301 1699 2219 Fragments arranged by size: 2219 1699 979 948 744 617 301 BsaAI yAC'GTr Cuts at: 493 1204 4672 5298 493 Size: 711 3468 626 2702 Fragments arranged by size: 3468 2702 711 626 BsaBI GATnn'nnATC Cuts at: 2966 7501 2966 Size: 4535 2972 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2988 3297 3459 4239 Size: 53 83 186 936 1455 309 162 780 Cuts at: 4239 4571 5314 5696 6331 275 Size: 332 743 382 635 1451 Fragments arranged by size: 1455 1451 936 780 743 635 382 332 309 186 162 83 53 BsaWI w'CCGG_w Cuts at: 2252 4384 5940 6338 6612 6759 7170 2252 Size: 2132 1556 398 274 147 411 2589 Fragments arranged by size: 2589 2132 1556 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 3171 3201 1178 Size: 30 165 30 1176 30 562 30 5484 Fragments arranged by size: 5484 1176 562 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2303 1186 Size: 852 265 6390 Fragments arranged by size: 6390 852 265 BseYI C'CCAG_C Cuts at: 2668 3789 4756 6710 7206 2668 Size: 1121 967 1954 496 2969 Fragments arranged by size: 2969 1954 1121 967 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 3261 3857 4356 4431 1163 Size: 573 840 685 596 499 75 4239 Fragments arranged by size: 4239 840 685 596 573 499 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2275 4957 5718 6746 1540 Size: 275 186 274 2682 761 1028 2301 Fragments arranged by size: 2682 2301 1028 761 275 274 186 BsiHKAI G_wGCw'C Cuts at: 3007 3731 4202 4601 5568 5653 6724 3007 Size: 724 471 399 967 85 1071 3790 Fragments arranged by size: 3790 1071 967 724 471 399 85 BsmI GAATG_Cn' Cuts at: 3475 5018 5111 3475 Size: 1543 93 5871 Fragments arranged by size: 5871 1543 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3288 3288 Size: 7507 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3007 3427 3576 3635 3731 3810 4192 Size: 399 1217 420 149 59 96 79 382 Cuts at: 4192 4202 4224 4601 4677 4826 5568 5653 6724 Size: 10 22 377 76 149 742 85 1071 Cuts at: 6724 1391 Size: 2174 Fragments arranged by size: 2174 1217 1071 742 420 399 382 377 149 149 96 85 79 76 59 22 10 BspEI T'CCGG_A Cuts at: 7170 7170 Size: 7507 BspHI T'CATG_A Cuts at: 5396 7126 5396 Size: 1730 5777 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 3176 4390 4751 4862 844 Size: 1343 989 1214 361 111 3489 Fragments arranged by size: 3489 1343 1214 989 361 111 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2470 5394 1394 Size: 429 205 442 2924 3507 Fragments arranged by size: 3507 2924 442 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 3691 4717 4774 6002 6176 65 Size: 1183 1759 684 1026 57 1228 174 1396 Fragments arranged by size: 1759 1396 1228 1183 1026 684 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 2870 4514 4619 6151 6338 1700 Size: 110 1060 1644 105 1532 187 2869 Fragments arranged by size: 2869 1644 1532 1060 187 110 105 BsrGI T'GTAC_A Cuts at: 96 3075 96 Size: 2979 4528 BssHII G'CGCG_C Cuts at: 1925 2478 2602 2756 1925 Size: 553 124 154 6676 Fragments arranged by size: 6676 553 154 124 BssSI C'ACGA_G Cuts at: 5567 6579 5567 Size: 1012 6495 BstAPI GCAn_nnn'nTGC Cuts at: 794 4672 794 Size: 3878 3629 BstBI TT'CG_AA Cuts at: 6311 6311 Size: 7507 BstEII G'GTnAC_C Cuts at: 1184 3163 1184 Size: 1979 5528 BstXI CCAn_nnnn'nTGG Cuts at: 2019 4348 4567 2019 Size: 2329 219 4959 Fragments arranged by size: 4959 2329 219 BstYI r'GATC_y Cuts at: 1972 2112 2173 2812 3839 4837 5204 5589 5606 Size: 140 61 639 1027 998 367 385 17 Cuts at: 5606 7058 7144 7156 7502 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1027 998 639 385 367 346 140 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2445 4293 4626 513 Size: 552 939 90 351 1848 333 3394 Fragments arranged by size: 3394 1848 939 552 351 333 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3100 3639 4521 487 Size: 683 56 1874 539 882 3473 Fragments arranged by size: 3473 1874 882 683 539 56 BtsI GCAGTG_nn' Cuts at: 2303 3978 5023 5817 5837 2303 Size: 1675 1045 794 20 3973 Fragments arranged by size: 3973 1675 1045 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7472 DraI TTT'AAA Cuts at: 4811 4811 Size: 7507 DraIII CAC_nnn'GTG Cuts at: 3772 3772 Size: 7507 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6514 809 Size: 507 5198 1802 Fragments arranged by size: 5198 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3716 3763 4350 Size: 54 1425 50 275 18 1886 47 587 Cuts at: 4350 4954 5843 7472 8 Size: 604 889 1629 43 Fragments arranged by size: 1886 1629 1425 889 604 587 275 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4954 1537 Size: 275 3142 4090 Fragments arranged by size: 4090 3142 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5437 1353 Size: 4084 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2668 4564 6094 6468 6614 387 Size: 1064 138 1079 1896 1530 374 146 1280 Fragments arranged by size: 1896 1530 1280 1079 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3729 4200 4599 3729 Size: 471 399 6637 Fragments arranged by size: 6637 471 399 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2235 2348 2372 2618 2786 4038 Size: 393 409 194 113 24 246 168 1252 Cuts at: 4038 4343 5570 6167 6954 7222 1239 Size: 305 1227 597 787 268 1524 Fragments arranged by size: 1524 1252 1227 787 597 409 393 305 268 246 194 168 113 24 EcoNI CCTnn'n_nnAGG Cuts at: 3717 3717 Size: 7507 EcoO109I rG'GnC_Cy Cuts at: 2664 2825 3451 3703 4220 4221 4753 2664 Size: 161 626 252 517 1 532 5418 Fragments arranged by size: 5418 626 532 517 252 161 1 EcoRI G'AATT_C Cuts at: 4816 5194 4816 Size: 378 7129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2523 3238 2523 Size: 715 6792 FspI TGC'GCA Cuts at: 6013 6013 Size: 7507 HaeII r_GCGC'y Cuts at: 1678 2991 4422 4641 6654 1678 Size: 1313 1431 219 2013 2531 Fragments arranged by size: 2531 2013 1431 1313 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 2762 2794 3730 Size: 32 135 32 1084 32 74 32 936 Cuts at: 3730 3762 4366 4398 4583 4615 6148 6180 6222 Size: 32 604 32 185 32 1533 32 42 Cuts at: 6222 6254 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 1084 936 604 185 135 74 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4851 5097 669 Size: 988 281 2913 246 3079 Fragments arranged by size: 3079 2913 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7507 HpaI GTT'AAC Cuts at: 5097 5097 Size: 7507 KasI G'GCGC_C Cuts at: 2987 2987 Size: 7507 KpnI G_GTAC'C Cuts at: 3756 4832 3756 Size: 1076 6431 MfeI C'AATT_G Cuts at: 5106 5106 Size: 7507 MluI A'CGCG_T Cuts at: 7383 7383 Size: 7507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 2634 3372 6621 6805 1305 Size: 1272 57 738 3249 184 2007 Fragments arranged by size: 3249 2007 1272 738 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 3765 4352 10 Size: 54 1425 343 1933 587 3165 Fragments arranged by size: 3165 1933 1425 587 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 2789 3071 3659 4547 5465 5824 Size: 648 1536 87 282 588 888 918 359 Cuts at: 5824 5983 518 Size: 159 2042 Fragments arranged by size: 2042 1536 918 888 648 588 359 282 159 87 MspA1I CmG'CkG Cuts at: 1606 2350 3761 4318 4491 4733 4923 5600 6748 Size: 744 1411 557 173 242 190 677 1148 Cuts at: 6748 6993 1606 Size: 245 2120 Fragments arranged by size: 2120 1411 1148 744 677 557 245 242 190 173 NaeI GCC'GGC Cuts at: 2872 4621 2872 Size: 1749 5758 NarI GG'CG_CC Cuts at: 2988 2988 Size: 7507 NcoI C'CATG_G Cuts at: 513 1065 2004 4626 513 Size: 552 939 2622 3394 Fragments arranged by size: 3394 2622 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7507 NgoMIV G'CCGG_C Cuts at: 2870 4619 2870 Size: 1749 5758 NheI G'CTAG_C Cuts at: 1052 2219 1052 Size: 1167 6340 NotI GC'GGCC_GC Cuts at: 4954 4954 Size: 7507 NruI TCG'CGA Cuts at: 2159 2159 Size: 7507 NspI r_CATG'y Cuts at: 1132 1229 4865 6410 1132 Size: 97 3636 1545 2229 Fragments arranged by size: 3636 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4606 1348 Size: 3258 4249 PciI A'CATG_T Cuts at: 6406 6406 Size: 7507 PflMI CCAn_nnn'nTGG Cuts at: 1108 4763 1108 Size: 3655 3852 PfoI T'CCnGG_A Cuts at: 2115 2379 2763 4426 2115 Size: 264 384 1663 5196 Fragments arranged by size: 5196 1663 384 264 PmeI GTTT'AAAC Cuts at: 4811 4811 Size: 7507 PmlI CAC'GTG Cuts at: 4672 4672 Size: 7507 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5576 5608 7089 7121 5576 Size: 32 1481 32 5962 Fragments arranged by size: 5962 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2664 3451 3703 2664 Size: 787 252 6468 Fragments arranged by size: 6468 787 252 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7507 PsiI TTA'TAA Cuts at: 5077 5077 Size: 7507 PspOMI G'GGCC_C Cuts at: 4220 4220 Size: 7507 PspXI vC'TCGA_Gb Cuts at: 3747 3747 Size: 7507 PstI C_TGCA'G Cuts at: 830 4668 4859 830 Size: 3838 191 3478 Fragments arranged by size: 3838 3478 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7507 PvuII CAG'CTG Cuts at: 3761 4733 3761 Size: 972 6535 SacI G_AGCT'C Cuts at: 3731 4202 4601 3731 Size: 471 399 6637 Fragments arranged by size: 6637 471 399 SalI G'TCGA_C Cuts at: 1936 4849 1936 Size: 2913 4594 SanDI GG'GwC_CC Cuts at: 2664 3451 2664 Size: 787 6720 SbfI CC_TGCA'GG Cuts at: 4859 4859 Size: 7507 ScaI AGT'ACT Cuts at: 3892 5755 7467 3892 Size: 1863 1712 3932 Fragments arranged by size: 3932 1863 1712 SexAI A'CCwGG_T Cuts at: 4330 4330 Size: 7507 SfcI C'TryA_G Cuts at: 826 1045 3826 4393 4664 4855 5990 6671 6862 Size: 219 2781 567 271 191 1135 681 191 Cuts at: 6862 826 Size: 1471 Fragments arranged by size: 2781 1471 1135 681 567 271 219 191 191 SfoI GGC'GCC Cuts at: 2989 2989 Size: 7507 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 SgrAI Cr'CCGG_yG Cuts at: 1700 2870 1700 Size: 1170 6337 SmaI CCC'GGG Cuts at: 1872 3421 4055 4834 1872 Size: 1549 634 779 4545 Fragments arranged by size: 4545 1549 779 634 SmlI C'TyrA_G Cuts at: 820 1017 1943 2471 3212 3747 5611 6512 6774 Size: 197 926 528 741 535 1864 901 262 Cuts at: 6774 820 Size: 1553 Fragments arranged by size: 1864 1553 926 901 741 535 528 262 197 SnaBI TAC'GTA Cuts at: 493 5298 493 Size: 4805 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7507 SphI G_CATG'C Cuts at: 1132 4865 1132 Size: 3733 3774 SspI AAT'ATT Cuts at: 5 52 5431 5 Size: 47 5379 2081 Fragments arranged by size: 5379 2081 47 StuI AGG'CCT Cuts at: 1517 2018 4254 1517 Size: 501 2236 4770 Fragments arranged by size: 4770 2236 501 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 4626 4946 513 Size: 552 939 160 2462 320 3074 Fragments arranged by size: 3074 2462 939 552 320 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2628 3937 4397 5534 5551 5704 5889 Size: 1255 1048 1309 460 1137 17 153 185 Cuts at: 5889 6334 325 Size: 445 1498 Fragments arranged by size: 1498 1309 1255 1137 1048 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3075 3890 5753 Size: 274 80 33 51 1443 1098 815 1863 Cuts at: 5753 7465 96 Size: 1712 138 Fragments arranged by size: 1863 1712 1443 1098 815 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3171 3203 7467 7499 3171 Size: 32 4264 32 3179 Fragments arranged by size: 4264 3179 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7507 XbaI T'CTAG_A Cuts at: 4172 4843 4172 Size: 671 6836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3244 3244 Size: 7507 XhoI C'TCGA_G Cuts at: 1943 2471 3747 1943 Size: 528 1276 5703 Fragments arranged by size: 5703 1276 528 XmaI C'CCGG_G Cuts at: 1870 3419 4053 4832 1870 Size: 1549 634 779 4545 Fragments arranged by size: 4545 1549 779 634 XmnI GAAnn'nnTTC Cuts at: 2135 3221 3307 5636 2135 Size: 1086 86 2329 4006 Fragments arranged by size: 4006 2329 1086 86 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5315 276 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NsiI PacI PsrI RsrII SacII SapI SfiI SrfI SwaI