(Circular) (Six-base) MAPSORT of: pFN21AE6329.seq Check: 3021 from: 1 to: 5797 pFN21AE6329 5797 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 3860 5244 5512 1632 Size: 710 1518 1384 268 1917 Fragments arranged by size: 1917 1518 1384 710 268 AfeI AGC'GCT Cuts at: 2865 2865 Size: 5797 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2400 4696 5673 1203 Size: 1197 2296 977 1327 Fragments arranged by size: 2296 1327 1197 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 2139 4526 2139 Size: 2387 3410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2492 2524 2492 Size: 32 5765 AlwNI CAG_nnn'CTG Cuts at: 2306 2437 3040 3540 5112 2306 Size: 131 603 500 1572 2991 Fragments arranged by size: 2991 1572 603 500 131 ApaI G_GGCC'C Cuts at: 2045 2045 Size: 5797 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 2627 3118 4573 618 Size: 325 217 627 529 311 491 1455 1842 Fragments arranged by size: 1842 1455 627 529 491 325 311 217 BanII G_rGCy'C Cuts at: 1391 2045 2082 2179 2244 2619 2748 3116 1391 Size: 654 37 97 65 375 129 368 4072 Fragments arranged by size: 4072 654 375 368 129 97 65 37 BbeI G_GCGC'C Cuts at: 2320 2320 Size: 5797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2245 928 Size: 971 346 4480 Fragments arranged by size: 4480 971 346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2722 2756 3988 4022 1903 Size: 34 50 34 701 34 1232 34 3678 Fragments arranged by size: 3678 1232 701 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2606 2880 4408 Size: 107 122 71 631 605 934 274 1528 Cuts at: 4408 136 Size: 1525 Fragments arranged by size: 1528 1525 934 631 605 274 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2183 2333 3214 2183 Size: 150 881 4766 Fragments arranged by size: 4766 881 150 Bme1580I G_kGCm'C Cuts at: 1790 2045 3858 5014 1790 Size: 255 1813 1156 2573 Fragments arranged by size: 2573 1813 1156 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2888 2899 3528 Size: 416 53 83 399 24 1471 11 629 Cuts at: 3528 4486 442 Size: 958 1753 Fragments arranged by size: 1753 1471 958 629 416 399 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 4457 1239 Size: 857 2361 2579 Fragments arranged by size: 2579 2361 857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2403 3588 493 Size: 711 1199 1185 2702 Fragments arranged by size: 2702 1199 1185 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2317 3604 3986 4621 Size: 53 83 186 936 784 1287 382 635 Cuts at: 4621 275 Size: 1451 Fragments arranged by size: 1451 1287 936 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2115 2637 4230 4628 4902 5049 5460 2115 Size: 522 1593 398 274 147 411 2452 Fragments arranged by size: 2452 1593 522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4453 Fragments arranged by size: 4453 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2540 2939 1186 Size: 856 498 399 4044 Fragments arranged by size: 4044 856 498 399 BseYI C'CCAG_C Cuts at: 2093 2103 2306 2455 2601 2866 5000 5496 2093 Size: 10 203 149 146 265 2134 496 2394 Fragments arranged by size: 2394 2134 496 265 203 149 146 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2425 1163 Size: 573 689 4535 Fragments arranged by size: 4535 689 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2994 3858 3943 5014 2082 Size: 912 864 85 1071 2865 Fragments arranged by size: 2865 1071 912 864 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 BsmBI CGTCTCn'nnnn_ Cuts at: 2921 2921 Size: 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2082 2179 2244 2619 2748 2994 Size: 399 255 37 97 65 375 129 246 Cuts at: 2994 3116 3858 3943 5014 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 255 246 129 122 97 85 65 37 BspEI T'CCGG_A Cuts at: 2115 2637 5460 2115 Size: 522 2823 2452 Fragments arranged by size: 2823 2452 522 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 3152 844 Size: 1832 476 3489 Fragments arranged by size: 3489 1832 476 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2194 3684 1394 Size: 429 364 7 1490 3507 Fragments arranged by size: 3507 1490 429 364 7 BsrDI GCAATG_nn' Cuts at: 65 1248 2368 2469 4292 4466 65 Size: 1183 1120 101 1823 174 1396 Fragments arranged by size: 1823 1396 1183 1120 174 101 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2605 2819 4441 4628 1700 Size: 110 297 498 214 1622 187 2869 Fragments arranged by size: 2869 1622 498 297 214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 2380 2437 3040 794 Size: 1586 57 603 3551 Fragments arranged by size: 3551 1586 603 57 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2208 2778 3127 3494 3879 3896 5348 5434 Size: 236 570 349 367 385 17 1452 86 Cuts at: 5434 5446 5792 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 349 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2631 2698 2757 3020 513 Size: 552 1566 67 59 263 3290 Fragments arranged by size: 3290 1566 552 263 67 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2959 487 Size: 683 56 1733 3325 Fragments arranged by size: 3325 1733 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2559 3313 4107 4127 2061 Size: 498 754 794 20 3731 Fragments arranged by size: 3731 794 754 498 20 ClaI AT'CG_AT Cuts at: 1554 2685 1554 Size: 1131 4666 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 2760 3244 Size: 54 1425 50 275 18 843 87 484 Cuts at: 3244 4133 5762 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 843 484 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 3006 3092 3727 1353 Size: 707 946 86 635 3423 Fragments arranged by size: 3423 946 707 635 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2080 2080 Size: 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2342 3860 4457 5244 5512 1239 Size: 393 464 246 1518 597 787 268 1524 Fragments arranged by size: 1524 1518 787 597 464 393 268 246 EcoO109I rG'GnC_Cy Cuts at: 2310 2542 2872 2941 3016 2310 Size: 232 330 69 75 5091 Fragments arranged by size: 5091 330 232 75 69 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2320 2412 2867 2886 3028 4944 1678 Size: 642 92 455 19 142 1916 2531 Fragments arranged by size: 2531 1916 642 455 142 92 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2125 2157 4438 4470 4512 Size: 32 135 32 553 32 2281 32 42 Cuts at: 4512 4544 1373 Size: 32 2626 Fragments arranged by size: 2626 2281 553 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2316 2316 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 4911 5095 1305 Size: 1173 2433 184 2007 Fragments arranged by size: 2433 2007 1173 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 2762 10 Size: 54 1425 343 843 87 3045 Fragments arranged by size: 3045 1425 843 343 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4114 4273 518 Size: 648 2589 359 159 2042 Fragments arranged by size: 2589 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2329 2455 2655 3040 3213 3890 5038 5283 Size: 723 126 200 385 173 677 1148 245 Cuts at: 5283 1606 Size: 2120 Fragments arranged by size: 2120 1148 723 677 385 245 200 173 126 NaeI GCC'GGC Cuts at: 2109 2607 2821 2109 Size: 498 214 5085 Fragments arranged by size: 5085 498 214 NarI GG'CG_CC Cuts at: 2317 2317 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 2757 513 Size: 552 1692 3553 Fragments arranged by size: 3553 1692 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NgoMIV G'CCGG_C Cuts at: 2107 2605 2819 2107 Size: 498 214 5085 Fragments arranged by size: 5085 498 214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 2344 1348 Size: 996 4801 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 2704 2791 1108 Size: 1488 108 87 4114 Fragments arranged by size: 4114 1488 108 87 PfoI T'CCnGG_A Cuts at: 2511 2511 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PmlI CAC'GTG Cuts at: 2403 2403 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2492 2524 3866 3898 5379 5411 2492 Size: 32 1342 32 1481 32 2878 Fragments arranged by size: 2878 1481 1342 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2941 3016 2941 Size: 75 5722 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2041 2041 Size: 5797 PstI C_TGCA'G Cuts at: 830 2442 2614 3036 3149 830 Size: 1612 172 422 113 3478 Fragments arranged by size: 3478 1612 422 172 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2329 2455 3040 2329 Size: 126 585 5086 Fragments arranged by size: 5086 585 126 SacI G_AGCT'C Cuts at: 2082 2082 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SapI GCTCTTCn'nnn_ Cuts at: 3092 3092 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2438 2610 3032 3145 4280 4961 5152 Size: 219 1393 172 422 113 1135 681 191 Cuts at: 5152 826 Size: 1471 Fragments arranged by size: 1471 1393 1135 681 422 219 191 172 113 SfoI GGC'GCC Cuts at: 2318 2318 Size: 5797 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3901 4802 5064 820 Size: 197 926 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2045 2757 2913 2979 3236 513 Size: 552 980 712 156 66 257 3074 Fragments arranged by size: 3074 980 712 552 257 156 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 2844 3824 3841 3994 4179 4624 Size: 1255 1131 133 980 17 153 185 445 Cuts at: 4624 325 Size: 1498 Fragments arranged by size: 1498 1255 1131 980 445 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 2507 3926 2507 Size: 1419 4378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI