################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AclI AflII AscI AseI AsnI Asp700I AvrII BamHI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BstAPI BstBI BstEII BstPI ClaI DraI DrdI EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PmeI PpuMI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 1599 GGTACC G^GTACC AccIII 149 TCCGGA T^CCGGA AcsI 544 GAATTC [GA]^AATT[CT] Alw44I 121 GTGCAC G^TGCAC AlwI 618 GATCC GGATC(4/5) AocI 1467 CCTCAGG CC^T[ACGT]AGG ApaI 1534 GGGCCC GGGCC^C ApaLI 121 GTGCAC G^TGCAC ApoI 544 GAATTC [GA]^AATT[CT] Asp718I 1599 GGTACC G^GTACC BbrPI 1245 CACGTG CAC^GTG BcgI 1448 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 58 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1377 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1377 GCTCAGC GC^T[ACGT]AGC BsaI 833 GGTCTC GGTCTC(1/5) BseAI 149 TCCGGA T^CCGGA BseRI 1651 GAGGAG GAGGAG(10/8) BsiEI 273 CGGCCG CG[GA][CT]^CG BsiWI 1160 CGTACG C^GTACG BspEI 149 TCCGGA T^CCGGA BsrBI 1047 CCGCTC CCGCTC(-3/-3) BsrDI 1037 CATTGC GCAATG(2/0) BssSI 403 CTCGTG CACGAG(-5/-1) Bst1107I 1194 GTATAC GTA^TAC BstYI 617 AGATCC [GA]^GATC[CT] BstZ17I 1194 GTATAC GTA^TAC Bsu36I 1467 CCTCAGG CC^T[ACGT]AGG CelII 1377 GCTCAGC GC^T[ACGT]AGC CpoI 151 CGGACCG CG^G[AT]CCG DpnII 618 GATC ^GATC EagI 273 CGGCCG C^GGCCG EclXI 273 CGGCCG C^GGCCG Eco52I 273 CGGCCG C^GGCCG Eco81I 1467 CCTCAGG CC^T[ACGT]AGG EcoRI 544 GAATTC G^AATTC EcoT14I 383 CCATGG C^C[AT][AT]GG KpnI 1599 GGTACC GGTAC^C KspI 227 CCGCGG CCGC^GG MboI 618 GATC ^GATC MflI 617 AGATCC [GA]^GATC[CT] MroI 149 TCCGGA T^CCGGA NcoI 383 CCATGG C^CATGG NdeII 618 GATC ^GATC NotI 272 GCGGCCGC GC^GGCCGC NspI 1502 ACATGC [GA]CATG^[CT] PflMI 1134 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1245 CACGTG CAC^GTG PmlI 1245 CACGTG CAC^GTG PstI 481 CTGCAG CTGCA^G RsrII 151 CGGACCG CG^G[AT]CCG SacII 227 CCGCGG CCGC^GG Sau3AI 618 GATC ^GATC SfcI 481 CTGCAG C^T[GA][CT]AG SgrAI 1623 CACCGGTG C[GA]^CCGG[CT]G SpeI 1401 ACTAGT A^CTAGT StuI 717 AGGCCT AGG^CCT StyI 383 CCATGG C^C[AT][AT]GG TfiI 711 GATTC G^A[AT]TC Tsp45I 1169 GTGAC ^GT[GC]AC Tsp509I 545 AATT ^AATT Van91I 1134 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1064 CCAAGAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 617 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 43 GTCTAC GT^[AC][GT]AC 1194 GTATAC AflIII 1244 ACACGT A^C[GA][CT]GT 1246 ACGTGT AgeI 1596 ACCGGT A^CCGGT 1624 ACCGGT AlwNI 604 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1470 CAGGGCCTG Aor51HI 695 AGCGCT AGC^GCT 1290 AGCGCT AviII 600 TGCGCA TGC^GCA 746 TGCGCA BalI 236 TGGCCA TGG^CCA 797 TGGCCA BsaAI 1162 TACGTG [CT]AC^GT[GA] 1245 CACGTG BsmAI 635 GAGAC GTCTC(1/5) 834 GTCTC BspMI 740 ACCTGC ACCTGC(4/8) 162 GCAGGT BsrFI 1596 ACCGGT [GA]^CCGG[CT] 1624 ACCGGT Cfr10I 1596 ACCGGT [GA]^CCGG[CT] 1624 ACCGGT DraIII 1135 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1360 CACCCCGTG Eco47III 695 AGCGCT AGC^GCT 1290 AGCGCT EcoNI 438 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1386 CCTCTTCAAGG FspI 600 TGCGCA TGC^GCA 746 TGCGCA MaeIII 992 GTTAC ^GT[ACGT]AC 1169 GTGAC MluNI 236 TGGCCA TGG^CCA 797 TGGCCA MscI 236 TGGCCA TGG^CCA 797 TGGCCA PinAI 1596 ACCGGT A^CCGGT 1624 ACCGGT PleI 306 GACTC GAGTC(4/5) 1255 GACTC PshAI 87 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 924 GACCAAAGTC PvuII 607 CAGCTG CAG^CTG 1449 CAGCTG SapI 1123 GAAGAGC GCTCTTC(1/4) 786 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AspEI AspHI AspI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PflFI RsaI SacI Sau96I ScrFI SfaNI SfoI SmlI TaqI TseI TspRI Tth111I TthHB8I